Common species affects the utility of non-invasive genetic monitoring of a cryptic endangered mammal: The bridled nailtail wallaby

2014 ◽  
Vol 39 (6) ◽  
pp. 633-642 ◽  
Author(s):  
Susan Nuske ◽  
Diana Fisher ◽  
Jennifer Seddon

PLoS ONE ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. e0227333 ◽  
Author(s):  
Raman P. Nagarajan ◽  
Alisha Goodbla ◽  
Emily Graves ◽  
Melinda Baerwald ◽  
Marcel Holyoak ◽  
...  


BMC Ecology ◽  
2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Hanna Granroth-Wilding ◽  
Craig Primmer ◽  
Meri Lindqvist ◽  
Jenni Poutanen ◽  
Olaf Thalmann ◽  
...  


2021 ◽  
Vol 67 (6) ◽  
Author(s):  
Francesco Lugli ◽  
Romolo Caniglia ◽  
Luca Mattioli ◽  
Elena Fabbri ◽  
Marco Mencucci ◽  
...  


2019 ◽  
Vol 39 (6) ◽  
pp. 409-415 ◽  
Author(s):  
Ana F.M. Botelho ◽  
Julliane V. Joviano-Santos ◽  
Artur Santos-Miranda ◽  
José E.R. Menezes-Filho ◽  
Benito Soto-Blanco ◽  
...  

ABSTRACT: Rats and mice are the most common species used in experimental cardiac electrophysiology studies. Electrocardiogram (ECG) recording shows paramount importance for monitoring arrhythmias and cardiac function in several disease models, including QT syndrome. However, the lack of standardized reference values and QT correction formula for different animal species and lineages represent a challenge for ECG interpretation. The aim of this study is to provide an improved method for ECG recording, establishing reference range values and determine the QT formulas with higher correlation to heart rate (HR). A total of 10 Wistar rats, 10 Swiss mice, 10 C57BL/6 mice and 10 FVB/NJ mice were used in the study. Animals were submitted to anesthesia with isoflurane and ECG recording was performed using a six-channel non-invasive electrocardiograph. QT was corrected using the following formulas: Bazzett, Fridericia, Mitchell, Hodges, Van der Water and Framingham. Normal range values for ECG parameters were established in all animals studied. Pearsons’ correlation defined Hodges formula as the most suitable for QT correction. This study demonstrated an improved method of ECG recording with reference values for Swiss, FVB/NJ, C57BL/6 mice, and Wistar rats. Hodges’ formula was the most effective formula for QT correction in rodents, whereas Bazett’s and Friderica formulas were ineffective for such animals. The present work contributes to arrhythmias investigation in experimental cardiology and may reduce misinterpretations in rodents’ ECG.



2012 ◽  
Vol 64 (1) ◽  
pp. 321-335 ◽  
Author(s):  
Elena Fabbri ◽  
R. Caniglia ◽  
Nadia Mucci ◽  
H.P. Thomsen ◽  
K. Krag ◽  
...  

Single nucleotide polymorphisms (SNPs) which represent the most widespread source of sequence variation in genomes, are becoming a routine application in several fields such as forensics, ecology and conservation genetics. Their use, requiring short amplifications, may allow a more efficient genotyping of degraded DNA. We provide the first application of SNP genotyping in an Italian non-invasive genetic monitoring project of the wolf. We compared three different techniques for genotyping SNPs: pyrosequencing, SNaPshot? and TaqMan? Probe Assay in Real-Time PCR. We successively genotyped nine SNPs using the TaqMan Probe Assay in 51 Italian wolves, 57 domestic dogs, 15 wolf x dog hybrids and 313 wolf scats collected in the northern Apennines. The obtained results were used to estimate genetic variability and PCR error rates in SNP genotyping protocols compared to standard microsatellite analysis. We evaluated the cost, laboratory effort and reliability of these different markers and discuss the possible future use of VeraCode, SNPlex and Fluidigm EP1 system in wild population monitoring.



2019 ◽  
Author(s):  
Anne Kempel ◽  
Hugo Vincent ◽  
Daniel Prati ◽  
Markus Fischer

AbstractAimBiotic interactions can determine rarity and commonness of species, however evidence that rare and common species respond differently to biotic stress is scarce. This is because biotic interactions are notoriously context-dependent and traits leading to success in one habitat might be costly or unimportant in another. We aim to identify plant characteristics that are related to biotic interactions and may drive patterns of rarity and commonness, taking environmental context into account.LocationSwitzerlandMethodsIn a multi-species experiment, we compared the response to biotic interactions of 19 rare and 21 widespread congeneric plant species in Switzerland, while also accounting for variation in environmental conditions of the species’ origin.ResultsOur results restrict the long-standing hypothesis that widespread species are superior competitors to rare species to only those species originating from resource rich habitats, in which competition is usually strong. Tolerance to herbivory and ambient herbivore damage on the other hand, did not differ between widespread and rare species. In accordance to the resource-availability hypothesis, widespread species from resource rich habitats where more damaged by herbivores (less defended) than widespread species from resource poor habitats – such a growth-defense tradeoff was lacking in rare species. This indicates that the evolutionary important tradeoff between traits increasing competitive-ability and defence is present in widespread species but may have been lost in rare species.Main conclusionsOur results indicate that biotic interactions, above all competition, might indeed set range limits, and underlines the importance of including context-dependency in studies comparing traits of common and rare or invasive and non-invasive species.



2016 ◽  
Vol 62 (2) ◽  
pp. 247-249
Author(s):  
Oddmund Kleven ◽  
Tomas Aarvak ◽  
Karl-Otto Jacobsen ◽  
Roar Solheim ◽  
Ingar J. Øien


2010 ◽  
Vol 47 (1) ◽  
pp. 172-181 ◽  
Author(s):  
Marta De Barba ◽  
Lisette P. Waits ◽  
Piero Genovesi ◽  
Ettore Randi ◽  
Roberta Chirichella ◽  
...  


2010 ◽  
Vol 143 (7) ◽  
pp. 1780-1791 ◽  
Author(s):  
M. Arandjelovic ◽  
J. Head ◽  
H. Kühl ◽  
C. Boesch ◽  
M.M. Robbins ◽  
...  


2015 ◽  
Vol 27 (1) ◽  
pp. 31-41 ◽  
Author(s):  
TR Wilbert ◽  
DA Woollett ◽  
A Whitelaw ◽  
J Dart ◽  
JR Hoyt ◽  
...  


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