A case report of pseudo‐exfoliation syndrome with prolonged corneal epithelial regeneration because of erlotinib‐induced unilateral corneal ulcer and anterior uveitis

AbstractForkhead box C1 (FOXC1) is required for neural crest and ocular development, and mutations in FOXC1 lead to inherited Axenfeld–Rieger syndrome. Here, we find that FOXC1 and paired box 6 (PAX6) are co-expressed in the human limbus and central corneal epithelium. Deficiency of FOXC1 and alternation in epithelial features occur in patients with corneal ulcers. FOXC1 governs the fate of the corneal epithelium by directly binding to lineage-specific open promoters or enhancers marked by H3K4me2. FOXC1 depletion not only activates the keratinization pathway and reprograms corneal epithelial cells into skin-like epithelial cells, but also disrupts the collagen metabolic process and interferon signaling pathways. Loss of interferon regulatory factor 1 and PAX6 induced by FOXC1 dysfunction is linked to the corneal ulcer. Collectively, our results reveal a FOXC1-mediated regulatory network responsible for corneal epithelial homeostasis and provide a potential therapeutic target for corneal ulcer.

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Transcriptional Network Analysis Reveals the Role of miR-223-5p During Diabetic Corneal Epithelial Regeneration

Frontiers in Molecular Biosciences ◽

10.3389/fmolb.2021.737472 ◽

2021 ◽

Vol 8 ◽

Author(s):

Yuan Zhang ◽

Shengqian Dou ◽

Xia Qi ◽

Zhenzhen Zhang ◽

Yujie Qiao ◽

...

Keyword(s):

Cell Proliferation ◽

Network Analysis ◽

Nerve Regeneration ◽

Corneal Epithelial ◽

Beneficial Effects ◽

Epithelial Regeneration ◽

Epithelial Wound Healing ◽

Direct Target Gene ◽

Inflammatory Processes

Diabetes mellitus (DM) is a complex metabolic disorder. Long-term hyperglycemia may induce diabetic keratopathy (DK), which is mainly characterized by delayed corneal epithelial regeneration. MicroRNAs (miRNAs) have been reported to play regulatory roles during tissue regeneration. However, the molecular mechanism by which miRNAs influence epithelial regeneration in DK is largely unknown. In this study, we performed miRNA and mRNA sequencing of regenerative corneal epithelium tissue from streptozotocin-induced type 1 diabetic (T1DM) and wild-type mice to screen for differentially expressed miRNAs and mRNAs. Based on regulatory network analysis, miR-223-5p was selected for subsequent experiments and Hpgds was then identified as a direct target gene. MiR-223-5p downregulation significantly promoted diabetic corneal epithelial wound healing and nerve regeneration. However, the beneficial effects of miR-223-5p inhibition were abolished by an Hpgds inhibitor. Furthermore, mechanistic studies demonstrated that miR-223-5p suppression ameliorated inflammation and enhanced cell proliferation signaling in DK. Taken together, our findings revealed that the regulatory role of miR-223-5p in diabetic corneal epithelial and nerve regeneration by mediating inflammatory processes and cell proliferation signaling. And silencing miR-223-5p may contribute to the development of potential therapeutic strategies for DK.

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Corneal ulcer due to a rare pleosporalean member of the genus Bipolaris following cow tail injury to the eye: A case report and review of literature

Indian Journal of Ophthalmology ◽

10.4103/ijo.ijo_836_16 ◽

2017 ◽

Vol 65 (5) ◽

pp. 403 ◽

Cited By ~ 2

Author(s):

HVijaya Pai ◽

Esha Jamal ◽

PrakashPeralam Yegneswaran

Keyword(s):

Case Report ◽

Corneal Ulcer ◽

Review Of Literature

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Long-term evaluation of autologous lamellar corneal grafts for the treatment of deep corneal ulcer in four dogs: a case report

Veterinární Medicína ◽

10.17221/139/2017-vetmed ◽

2019 ◽

Vol 64 (No. 02) ◽

pp. 84-91

Author(s):

SY Kim ◽

JY Kim ◽

SW Jeong

Keyword(s):

Case Report ◽

Corneal Ulcer ◽

Donor Site ◽

Ultrasound Biomicroscopy ◽

Corneal Graft ◽

Corneal Ulcers ◽

Term Evaluation ◽

The Aesthetic

Autologous lamellar corneal grafts were performed on four dogs with two deep corneal ulcers and two corneal perforations to achieve better visual and aesthetic outcomes. The donor corneal graft was harvested from the relatively normal corneal region of the affected eye and used to cover the corneal defect. At the two-year follow-up examination, ultrasound biomicroscopy was performed to confirm the thickness and endothelial continuity of the transplanted grafts and donor site of the cornea. The evaluations revealed that the dogs had healed without incident and that their vision had been maintained. Furthermore, the owners were satisfied with the aesthetic outcomes in all cases. Our findings show that autologous lamellar corneal grafts are useful and effective in treating canine corneal ulcers and perforations.

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