scholarly journals Statin‐boosted cellular uptake and endosomal escape of penetratin due to reduced membrane dipole potential

2021 ◽  
Author(s):  
Gyula Batta ◽  
Levente Kárpáti ◽  
Gabriela Fulaneto Henrique ◽  
Gabriella Tóth ◽  
Szabolcs Tarapcsák ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Endosomal Escape ◽  
Dipole Potential ◽  
Membrane Dipole Potential

scholarly journals Statin-boosted cellular uptake and endosomal escape of penetratin due to reduced membrane dipole potential

2020 ◽  
Author(s):  
Gyula Batta ◽  
Levente Karpati ◽  
Gabriela Fulaneto ◽  
Szabolcs Tarapcsak ◽  
Tamas Kovacs ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Endosomal Escape ◽  
Dipole Potential ◽  
Membrane Dipole Potential

scholarly journals Statin-boosted cellular uptake of penetratin due to reduced membrane dipole potential

2020 ◽  
Author(s):  
Gyula Batta ◽  
Levente Kárpáti ◽  
Gabriela Fulaneto Henrique ◽  
Szabolcs Tarapcsák ◽  
Tamás Kovács ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Cell Types ◽  
Cell Penetrating Peptides ◽  
Dipole Potential ◽  
Membrane Dipole Potential ◽  
Cell Penetrating ◽  
Ph Sensitive Dyes ◽  
Coa Reductase ◽  
Endocytic Compartments ◽  
Different Cell Types

AbstractSince cell penetrating peptides are promising tools for delivery of cargo into cells, factors limiting or facilitating their cellular uptake are intensely studied. Using labeling with pH-insensitive and pH-sensitive dyes we report that escape of penetratin from acidic endo-lysosomal compartments is retarded compared to its cellular uptake. The membrane dipole potential, known to alter transmembrane transport of charged molecules, is shown to be negatively correlated with the concentration of penetratin in the cytoplasmic compartment. Treatment of cells with therapeutically relevant concentrations of atorvastatin, an inhibitor of HMG-CoA reductase and cholesterol synthesis, significantly increased the release of penetratin from acidic endocytic compartments in two different cell types. This effect of atorvastatin correlated with its ability to decrease the membrane dipole potential. These results highlight the importance of the dipole potential in regulating cellular uptake of cell penetrating peptides and suggest a clinically relevant way of boosting this process.

Peptide-nucleic acid nanostructures for transfection

2012 ◽  
Vol 3 (3) ◽  
pp. 283-293 ◽  
Author(s):  
Burkhard Bechinger
Keyword(s):  
Nucleic Acid ◽  
Physicochemical Properties ◽  
Cellular Uptake ◽  
Peptide Nucleic Acid ◽  
Endosomal Escape ◽  
Medical Applications ◽  
Specific Cell ◽  
Charge Composition ◽  
Cell Surfaces ◽  
Intracellular Targeting

AbstractTo use nucleic acids in biomedical research and medical applications, these highly hydrophilic macromolecules have to be transported through the organism, targeted to specific cell surfaces, and have to cross cellular barriers. To this end, nanosized transfection complexes have been designed and several of them have been successfully tested. Here, the different steps of the transfection process and the particular optimization protocols are reviewed, including the physicochemical properties of such vectors (size, charge, composition), protection in serum, cellular uptake, endosomal escape, and intracellular targeting. The transfection process has been subdivided into separate steps and here special emphasis is given to peptides that have been designed to optimize these steps individually. Finally, complex devices encompassing a multitude of beneficial functionalities for transfection have been developed.

scholarly journals Amphotericin B Channel-Forming Activity Depends on Membrane Dipole Potential

2011 ◽  
Vol 100 (3) ◽  
pp. 133a
Author(s):  
Olga Ostroumova ◽  
Ludmila Schagina
Keyword(s):  
Amphotericin B ◽  
Dipole Potential ◽  
Membrane Dipole Potential

Effect of Cholesterol on the Interaction of the HIV GP41 Fusion Peptide with Model Membranes. Importance of the Membrane Dipole Potential†

Biochemistry ◽  
2006 ◽  
Vol 45 (51) ◽  
pp. 15768-15775 ◽  
Author(s):  
Víctor Buzón ◽  
Josep Cladera
Keyword(s):  
Fusion Peptide ◽  
Model Membranes ◽  
Dipole Potential ◽  
Membrane Dipole Potential ◽  
Hiv Gp41

scholarly journals Membrane dipole potential is sensitive to cholesterol stereospecificity: Implications for receptor function

2014 ◽  
Vol 184 ◽  
pp. 25-29 ◽  
Author(s):  
Suman Bandari ◽  
Hirak Chakraborty ◽  
Douglas F. Covey ◽  
Amitabha Chattopadhyay
Keyword(s):  
Receptor Function ◽  
Dipole Potential ◽  
Membrane Dipole Potential

Imidazole-Bearing Polymeric Micelles for Enhanced Cellular Uptake, Rapid Endosomal Escape, and On-demand Cargo Release

2018 ◽  
Vol 19 (6) ◽  
pp. 2610-2619 ◽  
Author(s):  
Di Lu ◽  
Yang An ◽  
Simin Feng ◽  
Xiaodan Li ◽  
Aiping Fan ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Polymeric Micelles ◽  
Endosomal Escape ◽  
On Demand

scholarly journals Differential Effect of Cholesterol and Its Biosynthetic Precursors on Membrane Dipole Potential

2012 ◽  
Vol 102 (7) ◽  
pp. 1561-1569 ◽  
Author(s):  
Sourav Haldar ◽  
Ravi Kumar Kanaparthi ◽  
Anunay Samanta ◽  
Amitabha Chattopadhyay
Keyword(s):  
Differential Effect ◽  
Dipole Potential ◽  
Membrane Dipole Potential

Mechanisms of cellular uptake and endosomal escape of calcium-siRNA nanocomplexes

2016 ◽  
Vol 515 (1-2) ◽  
pp. 46-56 ◽  
Author(s):  
Matan Goldshtein ◽  
Efrat Forti ◽  
Emil Ruvinov ◽  
Smadar Cohen
Keyword(s):  
Cellular Uptake ◽  
Endosomal Escape
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