Peptide-nucleic acid nanostructures for transfection

2012 ◽  
Vol 3 (3) ◽  
pp. 283-293 ◽  
Author(s):  
Burkhard Bechinger
Keyword(s):  
Nucleic Acid ◽  
Physicochemical Properties ◽  
Cellular Uptake ◽  
Peptide Nucleic Acid ◽  
Endosomal Escape ◽  
Medical Applications ◽  
Specific Cell ◽  
Charge Composition ◽  
Cell Surfaces ◽  
Intracellular Targeting

AbstractTo use nucleic acids in biomedical research and medical applications, these highly hydrophilic macromolecules have to be transported through the organism, targeted to specific cell surfaces, and have to cross cellular barriers. To this end, nanosized transfection complexes have been designed and several of them have been successfully tested. Here, the different steps of the transfection process and the particular optimization protocols are reviewed, including the physicochemical properties of such vectors (size, charge, composition), protection in serum, cellular uptake, endosomal escape, and intracellular targeting. The transfection process has been subdivided into separate steps and here special emphasis is given to peptides that have been designed to optimize these steps individually. Finally, complex devices encompassing a multitude of beneficial functionalities for transfection have been developed.

Synthesis and physicochemical properties of 20-mer peptide nucleic acid conjugates with testosterone 17β-carboxylic acid

2020 ◽  
Vol 61 (17) ◽  
pp. 151781
Author(s):  
Ryotaro Yagita ◽  
Kazuma Murakami ◽  
Hisafumi Ikeda ◽  
Kazuhiro Irie
Keyword(s):  
Nucleic Acid ◽  
Carboxylic Acid ◽  
Physicochemical Properties ◽  
Peptide Nucleic Acid

Cellular Uptake of Pyrrolidine-Based Oxy-Peptide Nucleic Acid

2010 ◽  
pp. 375-376
Author(s):  
Mizuki Kitamatsu ◽  
Rino Matsuzaki ◽  
Masahiko Sisido
Keyword(s):  
Nucleic Acid ◽  
Cellular Uptake ◽  
Peptide Nucleic Acid

Intracellular Behavior of Nanoparticles Based on their Physicochemical Properties

2015 ◽  
pp. 10-35 ◽  
Author(s):  
Charmainne Cruje ◽  
Darren Yohan ◽  
Celina Yang ◽  
Devika B. Chithrani
Keyword(s):  
Physicochemical Properties ◽  
Cellular Uptake ◽  
Treatment Options ◽  
Intracellular Localization ◽  
Disease Diagnosis ◽  
Current Treatment ◽  
Model System ◽  
Medical Applications ◽  
Gold Nps ◽  
Intracellular Pathway

This chapter addresses physicochemical properties that affect Nanoparticle (NP) intracellular behavior using Gold NPs (GNPs) as a model system. The main objective is to outline what is known about the effect of GNP size, shape, and surface properties on cellular uptake and intracellular pathway. The authors propose that the entry of GNPs into cells is related to its effectiveness in applications that favor intracellular localization of such GNPs. The authors also discuss how such properties are used to optimize GNP designs for medical applications. Finally, the authors discuss how GNPs may improve disease diagnosis and treatment. Furthermore, how they may be incorporated or used as alternatives to current treatment options is defined.

scholarly journals Cooperative Cellular Uptake and Activity of Octaarginine Antisense Peptide Nucleic acid (PNA) Conjugates

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 554 ◽  
Author(s):  
Ghavami ◽  
Shiraishi ◽  
Nielsen
Keyword(s):  
Cellular Uptake ◽  
Peptide Nucleic Acid ◽  
Peptide Nucleic Acids ◽  
Endosomal Escape ◽  
Cell Penetrating Peptide ◽  
Uptake Mechanism ◽  
Common Effect ◽  
Cell Penetrating ◽  
Antisense Peptide ◽  
Endosomal Uptake

Cellular uptake and antisense activity of d-octaarginine conjugated peptide nucleic acids (PNAs) is shown to exhibit pronounced cooperativity in serum-containing medium, in particular by being enhanced by analogous mis-match PNA–cell-penetrating peptide (PNA–CPP) conjugates without inherent antisense activity. This cooperativity does not show cell or PNA sequence dependency, suggesting that it is a common effect in cationic CPP conjugated PNA delivery. Interestingly, our results also indicate that Deca-r8-PNA and r8-PNA could assist each other and even other non-CPP PNAs as an uptake enhancer agent. However, the peptide itself (without being attached to the PNA) failed to enhance uptake and antisense activity. These results are compatible with an endosomal uptake mechanism in which the endocytosis event is induced by multiple CPP–PNA binding to the cell surface requiring a certain CPP density, possibly in terms of nanoparticle number and/or size, to be triggered. In particular the finding that the number of endosomal events is dependent on the total CPP–PNA concentration supports such a model. It is not possible from the present results to conclude whether endosomal escape is also cooperatively induced by CPP–PNA.

Intracellular Behavior of Nanoparticles Based on their Physicochemical Properties

2018 ◽  
pp. 1101-1127
Author(s):  
Charmainne Cruje ◽  
Darren Yohan ◽  
Celina Yang ◽  
Devika B. Chithrani
Keyword(s):  
Physicochemical Properties ◽  
Cellular Uptake ◽  
Treatment Options ◽  
Intracellular Localization ◽  
Disease Diagnosis ◽  
Current Treatment ◽  
Model System ◽  
Medical Applications ◽  
Gold Nps ◽  
Intracellular Pathway

This chapter addresses physicochemical properties that affect Nanoparticle (NP) intracellular behavior using Gold NPs (GNPs) as a model system. The main objective is to outline what is known about the effect of GNP size, shape, and surface properties on cellular uptake and intracellular pathway. The authors propose that the entry of GNPs into cells is related to its effectiveness in applications that favor intracellular localization of such GNPs. The authors also discuss how such properties are used to optimize GNP designs for medical applications. Finally, the authors discuss how GNPs may improve disease diagnosis and treatment. Furthermore, how they may be incorporated or used as alternatives to current treatment options is defined.

Molecular insights on the dynamic stability of peptide nucleic acid functionalized carbon and boron nitride nanotubes

2021 ◽  
Vol 23 (1) ◽  
pp. 219-228
Author(s):  
Nabanita Saikia ◽  
Mohamed Taha ◽  
Ravindra Pandey
Keyword(s):  
Nucleic Acid ◽  
Boron Nitride ◽  
Nucleic Acids ◽  
Dynamic Stability ◽  
Peptide Nucleic Acid ◽  
Rational Design ◽  
Peptide Nucleic Acids ◽  
Boron Nitride Nanotubes ◽  
Molecular Hybrids ◽  
Single Wall Nanotubes

The rational design of self-assembled nanobio-molecular hybrids of peptide nucleic acids with single-wall nanotubes rely on understanding how biomolecules recognize and mediate intermolecular interactions with the nanomaterial's surface.

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