Effect of Cholesterol on the Interaction of the HIV GP41 Fusion Peptide with Model Membranes. Importance of the Membrane Dipole Potential†

Biochemistry ◽  
2006 ◽  
Vol 45 (51) ◽  
pp. 15768-15775 ◽  
Author(s):  
Víctor Buzón ◽  
Josep Cladera
Keyword(s):  
Fusion Peptide ◽  
Model Membranes ◽  
Dipole Potential ◽  
Membrane Dipole Potential ◽  
Hiv Gp41

scholarly journals Membrane dipole potential is sensitive to cholesterol stereospecificity: Implications for receptor function

2014 ◽  
Vol 184 ◽  
pp. 25-29 ◽  
Author(s):  
Suman Bandari ◽  
Hirak Chakraborty ◽  
Douglas F. Covey ◽  
Amitabha Chattopadhyay
Keyword(s):  
Receptor Function ◽  
Dipole Potential ◽  
Membrane Dipole Potential

scholarly journals Differential Effect of Cholesterol and Its Biosynthetic Precursors on Membrane Dipole Potential

2012 ◽  
Vol 102 (7) ◽  
pp. 1561-1569 ◽  
Author(s):  
Sourav Haldar ◽  
Ravi Kumar Kanaparthi ◽  
Anunay Samanta ◽  
Amitabha Chattopadhyay
Keyword(s):  
Differential Effect ◽  
Dipole Potential ◽  
Membrane Dipole Potential

scholarly journals An ω-3, but Not an ω-6 Polyunsaturated Fatty Acid Decreases Membrane Dipole Potential and Stimulates Endo-Lysosomal Escape of Penetratin

2021 ◽  
Vol 9 ◽  
Author(s):  
Florina Zakany ◽  
Mate Szabo ◽  
Gyula Batta ◽  
Levente Kárpáti ◽  
István M. Mándity ◽  
...  
Keyword(s):  
Linolenic Acid ◽  
Measurement Techniques ◽  
Transmembrane Proteins ◽  
Living Cells ◽  
Cell Penetrating Peptide ◽  
Dipole Potential ◽  
Membrane Dipole Potential ◽  
Cell Penetrating ◽  
A Cell ◽  
Novel Method

Although the largely positive intramembrane dipole potential (DP) may substantially influence the function of transmembrane proteins, its investigation is deeply hampered by the lack of measurement techniques suitable for high-throughput examination of living cells. Here, we describe a novel emission ratiometric flow cytometry method based on F66, a 3-hydroxiflavon derivative, and demonstrate that 6-ketocholestanol, cholesterol and 7-dehydrocholesterol, saturated stearic acid (SA) and ω-6 γ-linolenic acid (GLA) increase, while ω-3 α-linolenic acid (ALA) decreases the DP. These changes do not correlate with alterations in cell viability or membrane fluidity. Pretreatment with ALA counteracts, while SA or GLA enhances cholesterol-induced DP elevations. Furthermore, ALA (but not SA or GLA) increases endo-lysosomal escape of penetratin, a cell-penetrating peptide. In summary, we have developed a novel method to measure DP in large quantities of individual living cells and propose ALA as a physiological DP lowering agent facilitating cytoplasmic entry of penetratin.

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