scholarly journals 20‐HETE is a pivotal endogenous ligand for TRPV1‐mediated neurogenic inflammation in the skin

2021 ◽  
Author(s):  
Alexander Hamers ◽  
Christopher P. Primus ◽  
Charlotte Whitear ◽  
Nitin Ajit Kumar ◽  
Michael Masucci ◽  
...  
Keyword(s):  
Neurogenic Inflammation ◽  
Endogenous Ligand

No release of histamine and substance P in capsaicin-induced neurogenic inflammation in intact human skin in vivo: a microdialysis study

1997 ◽  
Vol 27 (8) ◽  
pp. 957-965 ◽  
Author(s):  
L. J. PETERSEN ◽  
K. WINGE ◽  
E. BRODIN ◽  
P. S. SKOV
Keyword(s):  
Substance P ◽  
Human Skin ◽  
Neurogenic Inflammation ◽  
No Release ◽  
Microdialysis Study

Influence of loose ligation of the sciatic nerve on neurogenic inflammation

2000 ◽  
Vol 38 ◽  
pp. S117
Author(s):  
N Yonehara
Keyword(s):  
Sciatic Nerve ◽  
Neurogenic Inflammation

АКТУАЛЬНЫЕ АСПЕКТЫ ПРИМЕНЕНИЯ ПИМЕКРОЛИМУСА В КЛИНИЧЕСКОЙ ПРАКТИКЕ

2019 ◽  
Author(s):  
A.N. Lvov
Keyword(s):  
Atopic Dermatitis ◽  
Neurogenic Inflammation

В статье приводятся современные данные по патогенетической концепции нейрогенного воспаления при иммуноопосредованных дерматозах. На основании анализа литературы и собственного опыта освещены основные аспекты применения пимекролимуса при атопическом дерматите и других заболеваниях кожи.Modern aspects of patogenetic conception of neurogenic inflammation in immune associated dermatoses are presented in the article. On the basis of literature data and own experience important aspects of pimecrolimus application in atopic dermatitis and other dermatoses are reviewed.

Role of Peripheral Tachykinin Receptors in Neurogenic Inflammation of the Respiratory, Genitourinary and Gastrointestinal Systems

2003 ◽  
Vol 2 (2) ◽  
pp. 157-174 ◽  
Author(s):  
Stefano Evangelista ◽  
Riccardo Patacchini ◽  
Carlo Maggi
Keyword(s):  
Neurogenic Inflammation ◽  
Tachykinin Receptors

Evidence for presence of a reduced form of digoxin-like immunoreactive factor (dihydro-DLIF) in mammalian tissues

1996 ◽  
Vol 42 (7) ◽  
pp. 1092-1099 ◽  
Author(s):  
H M Qazzaz ◽  
S A Jortani ◽  
J M Poole ◽  
R Valdes
Keyword(s):  
Lactone Ring ◽  
Fold Increase ◽  
Cross Reactivity ◽  
Reduced Form ◽  
Molar Ratio ◽  
Endogenous Ligand ◽  
Metabolic Route ◽  
Mammalian Tissues ◽  
Spectral Absorbance ◽  
Cytochrome P 450

Abstract Digoxin-like immunoreactive factor (DLIF) from adrenal glands is an endogenous ligand structurally related to the plant-derived cardiac glycoside digoxin. Cardiac glycosides regulate the activity of the sodium pump and thus play key roles in disease processes involving regulation of ion transport. We now report the discovery of an endogenous dihydro-DLIF analogous to dihydrodigoxin. We used HPLC, ultraviolet spectrophotometry, and cross-reactivity with two antibodies, one specific for digoxin and one for dihydrodigoxin, to support the hypothesis that dihydro-DLIF contains a chemically reduced lactone ring. The spectral absorbance maximum for dihydro-DLIF is at 196 nm, identical to dihydrodigoxin. DLIF and dihydro-DLIF are 975- and 2588-fold less immunoreactive than digoxin and dihydrodigoxin for their respective antibodies. The molar ratio of dihydro-DLIF to DLIF is approximately 5.3 in bovine adrenocortical tissue and approximately 0.38 in human serum. Dihydrodigoxin (reduced lactone ring) added to microsomes isolated from bovine adrenal cortex produced a 4.5-fold increase in digoxin-like immunoreactivity (oxidized lactone ring) after 3 h of incubation. The biotransformation is likely mediated by a cytochrome P-450 NADPH-dependent process. Our findings demonstrate the presence of a dihydro-DLIF in mammals and suggest a metabolic route for synthesis of endogenous DLIF in mammalian tissue.

scholarly journals Structural basis for heme-dependent NCoR binding to the transcriptional repressor REV-ERBβ

2021 ◽  
Vol 7 (5) ◽  
pp. eabc6479
Author(s):  
Sarah A. Mosure ◽  
Timothy S. Strutzenberg ◽  
Jinsai Shang ◽  
Paola Munoz-Tello ◽  
Laura A. Solt ◽  
...  
Keyword(s):  
Nuclear Receptors ◽  
Binding Mode ◽  
Structural Basis ◽  
Receptor Interaction ◽  
Heme Binding ◽  
Endogenous Ligand ◽  
Interaction Domain ◽  
Response Elements ◽  
Biochemical Assays ◽  
Chemical Cross Linking

Heme is the endogenous ligand for the constitutively repressive REV-ERB nuclear receptors, REV-ERBα (NR1D1) and REV-ERBβ (NR1D2), but how heme regulates REV-ERB activity remains unclear. Cellular studies indicate that heme is required for the REV-ERBs to bind the corepressor NCoR and repress transcription. However, fluorescence-based biochemical assays suggest that heme displaces NCoR; here, we show that this is due to a heme-dependent artifact. Using ITC and NMR spectroscopy, we show that heme binding remodels the thermodynamic interaction profile of NCoR receptor interaction domain (RID) binding to REV-ERBβ ligand-binding domain (LBD). We solved two crystal structures of REV-ERBβ LBD cobound to heme and NCoR peptides, revealing the heme-dependent NCoR binding mode. ITC and chemical cross-linking mass spectrometry reveals a 2:1 LBD:RID stoichiometry, consistent with cellular studies showing that NCoR-dependent repression of REV-ERB transcription occurs on dimeric DNA response elements. Our findings should facilitate renewed progress toward understanding heme-dependent REV-ERB activity.

scholarly journals Estimating drug potency in the competitive target mediated drug disposition (TMDD) system when the endogenous ligand is included.

2021 ◽  
Author(s):  
Begum Alaybeyoglu ◽  
Ho Wa (Jacky) Cheng ◽  
Kshama A. Doshi ◽  
Vishruti Makani ◽  
Andrew M. Stein
Keyword(s):  
Drug Disposition ◽  
Endogenous Ligand ◽  
Drug Potency ◽  
Target Mediated Drug Disposition

scholarly journals Neurogenic inflammation induced by formalin

1984 ◽  
Vol 36 ◽  
pp. 235
Author(s):  
Manabu Shibata ◽  
Tsuyako Ohkubo ◽  
Hiroshi Takahashi ◽  
Satoru Naruse
Keyword(s):  
Neurogenic Inflammation
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