scholarly journals Peptide fragments of bradykinin show unexpected biological activity not mediated by B 1 or B 2 receptors

Author(s):  
Igor Maciel Souza‐Silva ◽  
Cristiane Amorim Paula ◽  
Lucas Bolais‐Ramos ◽  
Anderson Kenedy Santos ◽  
Filipe Alex Silva ◽  
...  
2014 ◽  
Vol 10 ◽  
pp. 155-162 ◽  
Author(s):  
Nadezhda V Sokolova ◽  
Valentine G Nenajdenko ◽  
Vladimir B Sokolov ◽  
Daria V Vinogradova ◽  
Elena F Shevtsova ◽  
...  

The synthesis of novel peptide conjugates of N-substituted-tetrahydro-γ-carbolines has been performed using the sequence of the Ugi multicomponent reaction and Cu(I)-catalyzed click chemistry. The effect of obtained γ-carboline–peptide conjugates on the rat liver mitochondria was evaluated. It was found that all compounds in the concentration of 30 µM did onot induce depolarization of mitochondria but possessed some inhibitory effect on the mitochondria permeability transition. The original N-substituted-tetrahydro-γ-carbolines containing an terminal alkyne group demonstrated a high prooxidant activity, whereas their conjugates with peptide fragments slightly inhibited both autooxidation and the t-BHP-induced lipid peroxidation.


Author(s):  
Igor Souza-Silva ◽  
Cristiane de Paula ◽  
Lucas Bolais-Ramos ◽  
Anderson Santos ◽  
Filipe da Silva ◽  
...  

Background and purpose: Bradykinin [BK-(1-9)] is an endogenous nonapeptide involved in multiple physiological and pathological processes. A long-held belief is that peptide fragments of BK-(1-9) are biologically inactive. Here, we have tested the biological activities of BK-(1-9) and two major peptide fragments in human and animal systems. Experimental Approach: Levels of BK peptides in male Wistar rat plasma were quantified by mass spectrometric methods. Nitric oxide was quantified in human, mouse and rat cells, and loaded with DAF-FM. We used aortic rings from adult male Wistar rats to test vascular reactivity. Changes in blood pressure and heart rate were measured in conscious adult male Wistar rats. Key results: Plasma levels of BK-(1-7) and BK-(1-5) in rats were increased following infusion of BK-(1-9). All tested peptides induced NO production in all cell types tested. However, unlike BK-(1-9), NO production elicited by BK-(1-7) or BK-(1-5) was not inhibited by B or B receptor antagonists. BK-(1-7) or BK-(1-5) also induced concentration-dependent vasorelaxation of aortic rings, without involving B or B receptors. In vivo, either intravenous or intra-arterial administration of BK-(1-7) or BK-(1-5) induced similar hypotension response. Conclusions and implications: BK-(1-7) and BK-(1-5) are endogenous peptides present in plasma. They are formed, at least partially, through the BK-(1-9) proteolysis. BK-related peptide fragments show biological activity, not mediated by B or B receptors. These BK-fragments could constitute new, active components of the kallikrein-kinin system.


1996 ◽  
Vol 215 (2) ◽  
pp. 127-130 ◽  
Author(s):  
Kosei Ojika ◽  
Yasuytzki Ueki ◽  
Shigehisa Mitake ◽  
Yusukuni Tsugu ◽  
Yasushi Otsuka ◽  
...  

1983 ◽  
Vol 115 (1) ◽  
pp. 193-200 ◽  
Author(s):  
Philip Gottlieb ◽  
Ester Tzehoval ◽  
Michael Feldman ◽  
Shraga Segal ◽  
Mati Fridkin

1977 ◽  
Vol 168 (1) ◽  
pp. 9-13 ◽  
Author(s):  
H P J Bennett ◽  
A M Hudson ◽  
C McMartin ◽  
G E Purdon

Peptides can be adsorbed on octadecasilyl-silica from large volumes of aqueous solution and eluted with aqueous solvent mixtures containing methanol or acetonitrile. These properties may be used for the extraction and purification of peptide fragments in plasma samples collected from rats. After intravenous injection of Synacthen [corticotropin-(1-24)-tetracosapeptide], it was shown that within 2 min the main circulating products were intact peptide and its sulphoxide. In addition, a number of fragments indicative of cleavage at the N- and C-termini were present. Most of the products formed from Synacthen have low biological activity. Somatostatin was rapidly cleaved in vivo and in vitro to a single product, which probably retains biological activity. The absence of other circulating products suggests that somatostatin is only inactivated once it leaves the circulation.


Author(s):  
G. Kasnic ◽  
S. E. Stewart ◽  
C. Urbanski

We have reported the maturation of an intracisternal A-type particle in murine plasma cell tumor cultures and three human tumor cell cultures (rhabdomyosarcoma, lung adenocarcinoma, and osteogenic sarcoma) after IUDR-DMSO activation. In all of these studies the A-type particle seems to develop into a form with an electron dense nucleoid, presumably mature, which is also intracisternal. A similar intracisternal A-type particle has been described in leukemic guinea pigs. Although no biological activity has yet been demonstrated for these particles, on morphologic grounds, and by the manner in which they develop within the cell, they may represent members of the same family of viruses.


Author(s):  
John L. Beggs ◽  
John D. Waggener ◽  
Wanda Miller

Microtubules (MT) are versatile organelles participating in a wide variety of biological activity. MT involvement in the movement and transport of cytoplasmic components has been well documented. In the course of our study on trauma-induced vasogenic edema in the spinal cord we have concluded that endothelial vesicles contribute to the edema process. Using horseradish peroxidase as a vascular tracer, labeled endothelial vesicles were present in all situations expected if a vesicular transport mechanism was in operation. Frequently,labeled vesicles coalesced to form channels that appeared to traverse the endothelium. The presence of MT in close proximity to labeled vesicles sugg ested that MT may play a role in vesicular activity.


2002 ◽  
Vol 23 (1) ◽  
pp. 79-121 ◽  
Author(s):  
Kathleen Taubert ◽  
Susanne Kraus ◽  
Bärbel Schulze

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