scholarly journals Peptide fragments of bradykinin show unexpected biological activity not mediated by B 1 or B 2 receptors

2022 ◽  
Author(s):  
Igor Maciel Souza‐Silva ◽  
Cristiane Amorim Paula ◽  
Lucas Bolais‐Ramos ◽  
Anderson Kenedy Santos ◽  
Filipe Alex Silva ◽  
...  
Keyword(s):  
Biological Activity ◽  
Peptide Fragments

scholarly journals Synthesis and biological activity of N-substituted-tetrahydro-γ-carbolines containing peptide residues

2014 ◽  
Vol 10 ◽  
pp. 155-162 ◽  
Author(s):  
Nadezhda V Sokolova ◽  
Valentine G Nenajdenko ◽  
Vladimir B Sokolov ◽  
Daria V Vinogradova ◽  
Elena F Shevtsova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Biological Activity ◽  
Permeability Transition ◽  
Liver Mitochondria ◽  
Inhibitory Effect ◽  
Rat Liver Mitochondria ◽  
Terminal Alkyne ◽  
Peptide Fragments ◽  
Prooxidant Activity ◽  
Peptide Conjugates

The synthesis of novel peptide conjugates of N-substituted-tetrahydro-γ-carbolines has been performed using the sequence of the Ugi multicomponent reaction and Cu(I)-catalyzed click chemistry. The effect of obtained γ-carboline–peptide conjugates on the rat liver mitochondria was evaluated. It was found that all compounds in the concentration of 30 µM did onot induce depolarization of mitochondria but possessed some inhibitory effect on the mitochondria permeability transition. The original N-substituted-tetrahydro-γ-carbolines containing an terminal alkyne group demonstrated a high prooxidant activity, whereas their conjugates with peptide fragments slightly inhibited both autooxidation and the t-BHP-induced lipid peroxidation.

scholarly journals Peptide fragments of bradykinin show unexpected biological activity not mediated by B1 or B2 receptors

2021 ◽  
Author(s):  
Igor Souza-Silva ◽  
Cristiane de Paula ◽  
Lucas Bolais-Ramos ◽  
Anderson Santos ◽  
Filipe da Silva ◽  
...  
Keyword(s):  
Biological Activity ◽  
Adult Male ◽  
Wistar Rats ◽  
Vascular Reactivity ◽  
Biological Activities ◽  
No Production ◽  
Peptide Fragments ◽  
Kinin System ◽  
Male Wistar Rats

Background and purpose: Bradykinin [BK-(1-9)] is an endogenous nonapeptide involved in multiple physiological and pathological processes. A long-held belief is that peptide fragments of BK-(1-9) are biologically inactive. Here, we have tested the biological activities of BK-(1-9) and two major peptide fragments in human and animal systems. Experimental Approach: Levels of BK peptides in male Wistar rat plasma were quantified by mass spectrometric methods. Nitric oxide was quantified in human, mouse and rat cells, and loaded with DAF-FM. We used aortic rings from adult male Wistar rats to test vascular reactivity. Changes in blood pressure and heart rate were measured in conscious adult male Wistar rats. Key results: Plasma levels of BK-(1-7) and BK-(1-5) in rats were increased following infusion of BK-(1-9). All tested peptides induced NO production in all cell types tested. However, unlike BK-(1-9), NO production elicited by BK-(1-7) or BK-(1-5) was not inhibited by B or B receptor antagonists. BK-(1-7) or BK-(1-5) also induced concentration-dependent vasorelaxation of aortic rings, without involving B or B receptors. In vivo, either intravenous or intra-arterial administration of BK-(1-7) or BK-(1-5) induced similar hypotension response. Conclusions and implications: BK-(1-7) and BK-(1-5) are endogenous peptides present in plasma. They are formed, at least partially, through the BK-(1-9) proteolysis. BK-related peptide fragments show biological activity, not mediated by B or B receptors. These BK-fragments could constitute new, active components of the kallikrein-kinin system.

Demonstration of the biological activity of peptide fragments related to human and rat hippocampal cholinergic neurostimulating peptide (HCNP)

1996 ◽  
Vol 215 (2) ◽  
pp. 127-130 ◽  
Author(s):  
Kosei Ojika ◽  
Yasuytzki Ueki ◽  
Shigehisa Mitake ◽  
Yusukuni Tsugu ◽  
Yasushi Otsuka ◽  
...  
Keyword(s):  
Biological Activity ◽  
Peptide Fragments

Peptide fragments from the tuftsin containing domain of immunoglobulin G synthesis and biological activity

1983 ◽  
Vol 115 (1) ◽  
pp. 193-200 ◽  
Author(s):  
Philip Gottlieb ◽  
Ester Tzehoval ◽  
Michael Feldman ◽  
Shraga Segal ◽  
Mati Fridkin
Keyword(s):  
Biological Activity ◽  
Immunoglobulin G ◽  
Peptide Fragments

scholarly journals Use of octadecasilyl-silica for the extraction and purification of peptides in biological samples. Application to the identification of circulating metabolites of corticotropin-(1-24)-tetracosapeptide and somatostatin in vivo

1977 ◽  
Vol 168 (1) ◽  
pp. 9-13 ◽  
Author(s):  
H P J Bennett ◽  
A M Hudson ◽  
C McMartin ◽  
G E Purdon
Keyword(s):  
Aqueous Solution ◽  
Biological Activity ◽  
Solvent Mixtures ◽  
Plasma Samples ◽  
Peptide Fragments ◽  
Single Product ◽  
Extraction And Purification ◽  
Aqueous Solvent

Peptides can be adsorbed on octadecasilyl-silica from large volumes of aqueous solution and eluted with aqueous solvent mixtures containing methanol or acetonitrile. These properties may be used for the extraction and purification of peptide fragments in plasma samples collected from rats. After intravenous injection of Synacthen [corticotropin-(1-24)-tetracosapeptide], it was shown that within 2 min the main circulating products were intact peptide and its sulphoxide. In addition, a number of fragments indicative of cleavage at the N- and C-termini were present. Most of the products formed from Synacthen have low biological activity. Somatostatin was rapidly cleaved in vivo and in vitro to a single product, which probably retains biological activity. The absence of other circulating products suggests that somatostatin is only inactivated once it leaves the circulation.

Virus-Like Particles in a Human Breast Cancer: Structural Similarity to Previously Reported Particles

1973 ◽  
Vol 31 ◽  
pp. 378-379
Author(s):  
G. Kasnic ◽  
S. E. Stewart ◽  
C. Urbanski
Keyword(s):  
Breast Cancer ◽  
Biological Activity ◽  
Human Breast Cancer ◽  
Osteogenic Sarcoma ◽  
Human Tumor ◽  
Structural Similarity ◽  
Cell Tumor ◽  
Human Breast ◽  
Human Tumor Cell ◽  
Type Particle

We have reported the maturation of an intracisternal A-type particle in murine plasma cell tumor cultures and three human tumor cell cultures (rhabdomyosarcoma, lung adenocarcinoma, and osteogenic sarcoma) after IUDR-DMSO activation. In all of these studies the A-type particle seems to develop into a form with an electron dense nucleoid, presumably mature, which is also intracisternal. A similar intracisternal A-type particle has been described in leukemic guinea pigs. Although no biological activity has yet been demonstrated for these particles, on morphologic grounds, and by the manner in which they develop within the cell, they may represent members of the same family of viruses.

Effects of Vincristine on Endothelial Microtubules in the Spinal Cord

1976 ◽  
Vol 34 ◽  
pp. 58-59
Author(s):  
John L. Beggs ◽  
John D. Waggener ◽  
Wanda Miller
Keyword(s):  
Spinal Cord ◽  
Biological Activity ◽  
Horseradish Peroxidase ◽  
Transport Mechanism ◽  
Vasogenic Edema ◽  
Vesicular Transport ◽  
Close Proximity

Microtubules (MT) are versatile organelles participating in a wide variety of biological activity. MT involvement in the movement and transport of cytoplasmic components has been well documented. In the course of our study on trauma-induced vasogenic edema in the spinal cord we have concluded that endothelial vesicles contribute to the edema process. Using horseradish peroxidase as a vascular tracer, labeled endothelial vesicles were present in all situations expected if a vesicular transport mechanism was in operation. Frequently,labeled vesicles coalesced to form channels that appeared to traverse the endothelium. The presence of MT in close proximity to labeled vesicles sugg ested that MT may play a role in vesicular activity.

Isothiazol-3(2 H )-ones, Part I: Synthesis, Reactions and Biological Activity

2002 ◽  
Vol 23 (1) ◽  
pp. 79-121 ◽  
Author(s):  
Kathleen Taubert ◽  
Susanne Kraus ◽  
Bärbel Schulze
Keyword(s):  
Biological Activity ◽  
Synthesis Reactions
Sign in / Sign up

Export Citation Format

Share Document