scholarly journals Non–heat inactivated autologous serum increases accuracy of in vitro CFSE lymphocyte proliferation test (LPT) for nickel

2020 ◽  
Vol 50 (6) ◽  
pp. 722-732
Author(s):  
Niels P. J. Graaf ◽  
Hetty J. Bontkes ◽  
Sanne Roffel ◽  
Cornelis J. Kleverlaan ◽  
Thomas Rustemeyer ◽  
...  
Keyword(s):  
Lymphocyte Proliferation ◽  
Autologous Serum ◽  
Lymphocyte Proliferation Test

Thymopentin-Loaded pH-Sensitive Chitosan Nanoparticles for Oral Administration: Preparation, Characterization, and Pharmacodynamics

2006 ◽  
Vol 6 (9) ◽  
pp. 2936-2944 ◽  
Author(s):  
Ai-Ping Zheng ◽  
Jian-Cheng Wang ◽  
Wan-Liang Lu ◽  
Xuan Zhang ◽  
Hua Zhang ◽  
...  
Keyword(s):  
Biological Activity ◽  
Zeta Potential ◽  
Lymphocyte Proliferation ◽  
Enzymatic Degradation ◽  
Encapsulation Efficiency ◽  
Chitosan Nanoparticles ◽  
Ph Sensitive ◽  
Oral Drug ◽  
Lymphocyte Proliferation Test

Thymopentin, a potent immunomodulating drug, was incorporated into pH-sensitive chitosan nanoparticles prepared by ionic gelation of chitosan with tripolyphosphate anions and then coated with Eudragit S100 to improve the stability and the oral bioavailability. Nanoparticles particle size and zeta potential were measured by photo correction spectroscopy and laser Dopper anemometry. Its morphology was examined by environment scan electron microscope. The encapsulation efficiency and the release in vitro were determined by HPLC. Enzymatic stabilization was expressed by the enzymatic degradation of aminopeptidase. Biological activity of TP5 loaded in nanoparticles was assayed by lymphocyte proliferation test in vitro and the immune function (CD4+/CD8+) of irradiated rat in vivo. The results obtained demonstrated that the average sizes of pH-sensitive chitosan nanoparticles were 175.6 ± 17 nm, the zeta potential was 28.44 ± 0.5 mV and the encapsulation efficiency was 76.70 ± 2.6%. The cumulative release percentages of thymopentin from the pH-sensitive nanoparticles were 24.65%, 41.01%, and 81.44% incubated in different medium, 0.1 N HCl, pH 5.0 PBS, and pH 7.4 PBS, respectively. The pH-sensitive chitosan nanoparticles could efficiently protect TP5 from enzymatic degradation and prolong the degradation half-time of TP5 from 1.5 min to 15 min. It was demonstrated from the lymphocyte proliferation test that the nanoparticle-encapsulated TP5 still kept its biological activity. In immunosuppression rats, the lowered T-lymphocyte subsets values were significantly increased and the raised CD4+/CD8+ ratio was evidently reduced. These results indicated that pH-sensitive chitosan nanoparticles may be used as the vector in oral drug delivery system for TP5.

C-Reactive Protein (CRP) Modulates Lymphocyte Proliferation VIA Affecting Activation Step

1988 ◽  
Vol 1 (3) ◽  
pp. 201-210 ◽  
Author(s):  
K. E. Wiktorowicz ◽  
M. Kotecki ◽  
S. H. Mackiewicz ◽  
J. Morkowski ◽  
M. Kapcinska ◽  
...  
Keyword(s):  
Lymphocyte Proliferation ◽  
Lymphocyte Activation ◽  
Inhibitory Effect ◽  
Receptor Expression ◽  
Autologous Serum ◽  
Reactive Protein ◽  
High Doses ◽  
Proliferation In Vitro ◽  
Foetal Bovine Serum

The effect of CRP on lymphocyte activation and proliferation in vitro was estimated on the basis of measurement of 3H-thymidine incorporation, analysis of distribution of subsequent cell divisions and analysis of IL-2 receptor expression. Low doses of CRP (less than 3μg/ml) stimulated and high doses (over 5μg/ml) inhibited lymphocyte activation, with little effect on proliferating lymphoblasts. The presence of foetal bovine serum or autologous serum modified CRP action on lymphocytes. The inhibitory effect of CRP on lymphocyte proliferation was similar in cultures stimulated by PHA or by allogeneic cells. On the basis of results obtained, CRP is considered a possible immunomodulator.

Food allergy type IV (delayed type) diagnosis in vitro by optimized lymphocyte proliferation test (LPT)ȁfirst experiences with budenoside as therapy

2003 ◽  
Vol 124 (4) ◽  
pp. A189
Author(s):  
Peter Janetschek ◽  
W. Mayer ◽  
Volker V. Baehr ◽  
F. Tiller
Keyword(s):  
Food Allergy ◽  
Lymphocyte Proliferation ◽  
Type Iv ◽  
Lymphocyte Proliferation Test

In vitro lymphocyte proliferation test in the diagnosis of oral mucosal hypersensitivity reactions to dental amalgam

1997 ◽  
Vol 26 (8) ◽  
pp. 362-366 ◽  
Author(s):  
Juhani Laine ◽  
Risto-Pekka Happonen ◽  
Olli Vainio ◽  
Kirsti Kalimo
Keyword(s):  
Lymphocyte Proliferation ◽  
Dental Amalgam ◽  
Hypersensitivity Reactions ◽  
Lymphocyte Proliferation Test

In vitro Effects of L-Ascorbic Acid and Acrylamide on Lymphocyte Proliferation in Young and Aged Mice

2010 ◽  
Vol 15 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Nam-Sung Kang ◽  
Suhk-Neung Pyo ◽  
Eun-Hwa Sohn
Keyword(s):  
Ascorbic Acid ◽  
Lymphocyte Proliferation ◽  
Aged Mice ◽  
In Vitro Effects

Subset-Specific Effects of Sex Hormones and Pituitary Gonadotropins on Human Lymphocyte Proliferation in Vitro

1993 ◽  
Vol 66 (3) ◽  
pp. 201-211 ◽  
Author(s):  
Balu H. Athreya ◽  
Jonathan Pletcher ◽  
Francesco Zulian ◽  
David B. Weiner ◽  
William V. Williams
Keyword(s):  
Sex Hormones ◽  
Human Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Specific Effects ◽  
Proliferation In Vitro

scholarly journals T lymphocyte-dependent B lymphocyte proliferative response to antigen. I Genetic restriction of the stimulation of B lymphocyte proliferation.

1981 ◽  
Vol 153 (4) ◽  
pp. 871-882 ◽  
Author(s):  
H Y Tse ◽  
J J Mond ◽  
W E Paul
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
B Lymphocyte ◽  
Antigen Specificity ◽  
Apparent Lack ◽  
The Face ◽  
Stimulation Of

For the purpose of examining more closely the interaction between T and B lymphocytes, we have developed an in vitro T lymphocyte-dependent B lymphocyte proliferation assay. Proliferation of B lymphocytes in response to antigen was found to depend on the presence of primed T lymphocytes; the B lymphocytes could be derived from nonprimed animals. It appears that these B cells were nonspecifically recruited to proliferate. This nonspecific recruitment, however, was found to be Ir-gene restricted in that B lymphocytes from B10.S mice, which are genetic nonresponders to the polymer Glu60-Ala30-Tyr10 (GAT), could not be stimulated by GAT-primed (responder X nonresponder) F1 T cells. The apparent lack of antigen specificity in the face of Ir gene-restricted T-B interaction may have important implications in our understanding of the recognition unit(s) on T lymphocytes.

In vitro effects of methadone on lymphocyte proliferation following a short-term treatment with methotrexate-loaded liposomes in a murine model of arthritis

2006 ◽  
Vol 164 ◽  
pp. S110-S111
Author(s):  
Maria Barca ◽  
Anne Marie Ciobanu ◽  
Dan Balalau ◽  
Daniela Luiza Baconi ◽  
Mihaela Ilie ◽  
...  
Keyword(s):  
Murine Model ◽  
Lymphocyte Proliferation ◽  
Term Treatment ◽  
Short Term ◽  
Short Term Treatment ◽  
In Vitro Effects

Decreased tobacco-glycoprotein-induced lymphocyte proliferation in vitro in pulmonary eosinophilic granuloma.

1995 ◽  
Vol 151 (1) ◽  
pp. 145-150 ◽  
Author(s):  
L H Youkeles ◽  
J N Grizzanti ◽  
Z Liao ◽  
C J Chang ◽  
D L Rosenstreich
Keyword(s):  
Lymphocyte Proliferation ◽  
Eosinophilic Granuloma ◽  
Proliferation In Vitro
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