scholarly journals Deficiency of filaggrin regulates endogenous cysteine protease activity, leading to impaired skin barrier function

2017 ◽  
Vol 42 (6) ◽  
pp. 622-631 ◽  
Author(s):  
X. W. Wang ◽  
J. J. Wang ◽  
D. Gutowska-Owsiak ◽  
M. Salimi ◽  
T. A. Selvakumar ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Barrier Function ◽  
Protease Activity ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Cysteine Protease Activity

Influence of skin penetration enhancers on skin barrier function and skin protease activity

2014 ◽  
Vol 51 ◽  
pp. 118-122 ◽  
Author(s):  
Diar Mohammed ◽  
Kazumasa Hirata ◽  
Jonathan Hadgraft ◽  
Majella E. Lane
Keyword(s):  
Barrier Function ◽  
Protease Activity ◽  
Skin Barrier ◽  
Skin Penetration ◽  
Penetration Enhancers ◽  
Skin Barrier Function

Skin Barrier Function Repair Is the Next Step in Atopic Dermatitis Treatment

2006 ◽  
Vol 37 (11) ◽  
pp. 19
Author(s):  
DAMIAN MCNAMARA
Keyword(s):  
Atopic Dermatitis ◽  
Barrier Function ◽  
Skin Barrier ◽  
Skin Barrier Function

Increased cysteine-protease activity in fecal supernatants from constipated IBS-C patients leads to occludin enzymatic degradation in colonic mucosa

2011 ◽  
Vol 49 (05) ◽  
Author(s):  
R Róka ◽  
A Annaházi ◽  
V Bezirard ◽  
H Eutamene ◽  
L Ferrier ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Protease Activity ◽  
Enzymatic Degradation ◽  
Colonic Mucosa ◽  
Cysteine Protease Activity

Fecal supernatants from constipated IBS patients increase colonic permeability in mice by occludin degradation linked to cysteine-protease activity

2011 ◽  
Vol 49 (05) ◽  
Author(s):  
A Annaházi ◽  
R Róka ◽  
M Leveque ◽  
H Eutamene ◽  
L Ferrier ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Protease Activity ◽  
Cysteine Protease Activity

Instrumental evaluation of skin barrier function and clinical outcomes during dupilumab treatment for atopic dermatitis: An observational study

2021 ◽  
Author(s):  
Antonio Cristaudo ◽  
Flavia Pigliacelli ◽  
Francesca Sperati ◽  
Diego Orsini ◽  
Norma Cameli ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Observational Study ◽  
Clinical Outcomes ◽  
Barrier Function ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Instrumental Evaluation

scholarly journals EGFR inhibitors switch keratinocytes from a proliferative to a differentiative phenotype affecting epidermal development and barrier function

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nicolas Joly-Tonetti ◽  
Thomas Ondet ◽  
Mario Monshouwer ◽  
Georgios N. Stamatas
Keyword(s):  
Growth Factor ◽  
Barrier Function ◽  
Kinase Inhibitors ◽  
Treatment Protocol ◽  
Growth Factor Receptor ◽  
Skin Barrier ◽  
Keratinocyte Differentiation ◽  
Skin Barrier Function ◽  
Epidermal Keratinocytes ◽  
Epidermal Structure

Abstract Background Cutaneous adverse drug reactions (CADR) associated with oncology therapy involve 45–100% of patients receiving kinase inhibitors. Such adverse reactions may include skin inflammation, infection, pruritus and dryness, symptoms that can significantly affect the patient’s quality of life. To prevent severe skin damages dose adjustment or drug discontinuation is often required, interfering with the prescribed oncology treatment protocol. This is particularly the case of Epidermal Growth Factor Receptor inhibitors (EGFRi) targeting carcinomas. Since the EGFR pathway is pivotal for epidermal keratinocytes, it is reasonable to hypothesize that EGFRi also affect these cells and therefore interfere with the epidermal structure formation and skin barrier function. Methods To test this hypothesis, the effects of EGFRi and Vascular Endothelial Growth Factor Receptor inhibitors (VEGFRi) at therapeutically relevant concentrations (3, 10, 30, 100 nM) were assessed on proliferation and differentiation markers of human keratinocytes in a novel 3D micro-epidermis tissue culture model. Results EGFRi directly affect basal keratinocyte growth, leading to tissue size reduction and switching keratinocytes from a proliferative to a differentiative phenotype, as evidenced by decreased Ki67 staining and increased filaggrin, desmoglein-1 and involucrin expression compared to control. These effects lead to skin barrier impairment, which can be observed in a reconstructed human epidermis model showing a decrease in trans-epidermal water loss rates. On the other hand, pan-kinase inhibitors mainly targeting VEGFR barely affect keratinocyte differentiation and rather promote a proliferative phenotype. Conclusions This study contributes to the mechanistic understanding of the clinically observed CADR during therapy with EGFRi. These in vitro results suggest a specific mode of action of EGFRi by directly affecting keratinocyte growth and barrier function.

scholarly journals Skin Barrier Function in Psoriasis and Atopic Dermatitis: Transepidermal Water Loss and Temperature as Useful Tools to Assess Disease Severity

2021 ◽  
Vol 10 (2) ◽  
pp. 359 ◽  
Author(s):  
Trinidad Montero-Vilchez ◽  
María-Victoria Segura-Fernández-Nogueras ◽  
Isabel Pérez-Rodríguez ◽  
Miguel Soler-Gongora ◽  
Antonio Martinez-Lopez ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Disease Severity ◽  
Water Loss ◽  
Barrier Function ◽  
Skin Barrier ◽  
Transepidermal Water Loss ◽  
Diagnostic Tools ◽  
Psoriatic Skin ◽  
Skin Barrier Function ◽  
Healthy Controls

Multiple diagnostic tools are used to evaluate psoriasis and atopic dermatitis (AD) severity, but most of them are based on subjective components. Transepidermal water loss (TEWL) and temperature are skin barrier function parameters that can be objectively measured and could help clinicians to evaluate disease severity accurately. Thus, the aims of this study are: (1) to compare skin barrier function between healthy skin, psoriatic skin and AD skin; and (2) to assess if skin barrier function parameters could predict disease severity. A cross-sectional study was designed, and epidermal barrier function parameters were measured. The study included 314 participants: 157 healthy individuals, 92 psoriatic patients, and 65 atopic dermatitis patients. TEWL was significantly higher, while stratum corneum hydration (SCH) (8.71 vs. 38.43 vs. 44.39 Arbitrary Units (AU)) was lower at psoriatic plaques than at uninvolved psoriatic skin and healthy controls. Patients with both TEWL > 13.85 g·m−2h−1 and temperature > 30.85 °C presented a moderate/severe psoriasis (psoriasis area severity index (PASI) ≥ 7), with a specificity of 76.3%. TEWL (28.68 vs. 13.15 vs. 11.60 g·m−2 h−1) and temperature were significantly higher, while SCH (25.20 vs. 40.95 vs. 50.73 AU) was lower at AD eczematous lesions than uninvolved AD skin and healthy controls. Patients with a temperature > 31.75 °C presented a moderate/severe AD (SCORing Atopic Dermatitis (SCORAD) ≥ 37) with a sensitivity of 81.8%. In conclusion, temperature and TEWL values may help clinicians to determine disease severity and select patients who need intensive treatment.

scholarly journals Glucocorticoid Receptor Signaling in Skin Barrier Function

Keratin ◽  
2018 ◽  
Author(s):  
Lisa M. Sevilla ◽  
Paloma Pérez
Keyword(s):  
Glucocorticoid Receptor ◽  
Barrier Function ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Receptor Signaling
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