scholarly journals Cover Image: Disassembly of the apical junctional complex during the transmigration of Leptospira interrogans across polarized renal proximal tubule epithelial cells (Cellular Microbiology 09/2021)

2021 ◽  
Vol 23 (9) ◽  
Author(s):  
Isabel Sebastián ◽  
Nobuhiko Okura ◽  
Bruno M. Humbel ◽  
Jun Xu ◽  
Idam Hermawan ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Proximal Tubule ◽  
Renal Proximal Tubule ◽  
Cover Image ◽  
Junctional Complex ◽  
Leptospira Interrogans ◽  
Apical Junctional Complex

QS394. The Genomic Analysis of the Heterogeneity Hypoxia Responses in Renal Proximal Tubule Epithelial Cells After Knock-Down of HIF-1α and HIF-2α

2008 ◽  
Vol 144 (2) ◽  
pp. 424
Author(s):  
Zhen Wang ◽  
Edwin H. Rodriguez ◽  
Michael T. Longaker ◽  
Patrick O. Brown ◽  
Jen-Tsan A. Chi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Proximal Tubule ◽  
Genomic Analysis ◽  
Renal Proximal Tubule ◽  
Knock Down

scholarly journals Regulation of PDZ domain-containing 1 (PDZK1) expression by hepatocyte nuclear factor-1α (HNF1α) in human kidney

2017 ◽  
Vol 313 (4) ◽  
pp. F973-F983 ◽  
Author(s):  
Katharina Prestin ◽  
Janine Hussner ◽  
Celio Ferreira ◽  
Isabell Seibert ◽  
Vivien Breitung ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Proximal Tubule ◽  
Pdz Domain ◽  
Human Kidney ◽  
Renal Proximal Tubule ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Hepatocyte Nuclear Factor 1Α ◽  
Adenoviral Transfer ◽  
Sirna Knockdown

In the renal proximal tubule the secretion and reabsorption of glomerularly filtrated compounds is realized by a functional network of uptake and efflux transporters. The activity and localization of several transporters expressed at the apical tubular membrane are regulated by the membrane-associated protein PDZ domain-containing 1 (PDZK1). We aimed to characterize the transcriptional regulation of this modulator of renal transport. Coexpression analyses of PDZK1 and putative regulators were performed using human kidney samples. Protein and mRNA expression of PDZK1 in renal proximal tubule epithelial cells after adenoviral transfer and siRNA knockdown of transcription factor hepatocyte nuclear factor-1α (HNF1α) was assessed by quantitative real-time PCR and Western blot analysis. Transactivation of the PDZK1 promoter was quantified in cell-based reporter gene assays. Subsequently, the binding of HNF1α to the PDZK1 promoter was verified by in silico analyses and chromatin immunoprecipitation assay. HNF1α positively regulated the promoter activity of PDZK1. Adenoviral overexpression of HNF1α in renal proximal tubule epithelial cells (RPTEC) increased PDZK1 mRNA and protein expression, whereas siRNA knockdown of HNF1α resulted in decreased expression of PDZK1. Our results show that HNF1α, which has previously been described as a modulator of several transporters of the renal transportosome, is also a key determinant of PDZK1 transcription.

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