scholarly journals Myeloma light chains induce epithelial-mesenchymal transition in human renal proximal tubule epithelial cells

2007 ◽  
Vol 23 (3) ◽  
pp. 860-870 ◽  
Author(s):  
M. Li ◽  
K. S. Hering-Smith ◽  
E. E. Simon ◽  
V. Batuman
Keyword(s):  
Epithelial Cells ◽  
Proximal Tubule ◽  
Epithelial Mesenchymal Transition ◽  
Renal Proximal Tubule ◽  
Light Chains ◽  
Mesenchymal Transition

scholarly journals Tacrolimus Modulates TGF-β Signaling to Induce Epithelial-Mesenchymal Transition in Human Renal Proximal Tubule Epithelial Cells

2016 ◽  
Vol 5 (5) ◽  
pp. 50 ◽  
Author(s):  
Jason Bennett ◽  
Hilary Cassidy ◽  
Craig Slattery ◽  
Michael Ryan ◽  
Tara McMorrow
Keyword(s):  
Epithelial Cells ◽  
Proximal Tubule ◽  
Epithelial Mesenchymal Transition ◽  
Renal Proximal Tubule ◽  
Mesenchymal Transition

Silencing megalin and cubilin genes inhibits myeloma light chain endocytosis and ameliorates toxicity in human renal proximal tubule epithelial cells

2008 ◽  
Vol 295 (1) ◽  
pp. F82-F90 ◽  
Author(s):  
Min Li ◽  
Saravanan Balamuthusamy ◽  
Eric E. Simon ◽  
Vecihi Batuman
Keyword(s):  
Epithelial Cells ◽  
Proximal Tubule ◽  
Light Chain ◽  
Epithelial To Mesenchymal Transition ◽  
Human Kidney ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Monocyte Chemoattractant Protein 1 ◽  
Mesenchymal Transition ◽  
Morphological Alterations

Using target-specific short interfering (si) RNAs, we silenced the tandem endocytic receptors megalin and cubilin genes in cultured human renal proximal tubule epithelial cells. Transfection by siRNA resulted in up to 90% suppression of both megalin and cubilin protein and mRNA expression. In HK-2 cells exposed to κ-light chain for up to 24 h, light chain endocytosis was reduced in either megalin- or cubilin-silenced cells markedly but incompletely. Simultaneous silencing of both the cubilin and megalin genes, however, resulted in near-complete inhibition of light chain endocytosis, as determined by measuring κ-light chain protein concentration in cell cytoplasm and by flow cytometry using FITC-labeled κ-light chain. In these cells, light chain-induced cytokine responses (interleukin-6 and monocyte chemoattractant protein-1) and epithelial-to-mesenchymal transition as well as the associated cellular and morphological alterations were also markedly suppressed. The results demonstrate that light chain endocytosis is predominantly mediated by the megalin-cubilin tandem endocytic receptor and identify endocytosis as a key step in light chain cytotoxicity. Blocking light chain endocytosis prevents its nephrotoxic effects on human kidney proximal tubule cells.

Epithelial-mesenchymal transition of biliary epithelial cells during cholestatic fibrosis

2007 ◽  
Vol 45 (01) ◽  
Author(s):  
F Schulze ◽  
E Konze ◽  
K Petmecky ◽  
K Schardt ◽  
O Dirsch ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Epithelial Mesenchymal Transition ◽  
Mesenchymal Transition ◽  
Biliary Epithelial Cells

scholarly journals Different macrophages equally induce EMT in endometria of adenomyosis and normal

Reproduction ◽  
2017 ◽  
Vol 154 (1) ◽  
pp. 79-92 ◽  
Author(s):  
Min An ◽  
Dong Li ◽  
Ming Yuan ◽  
Qiuju Li ◽  
Lu Zhang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Transforming Growth Factor ◽  
Epithelial Mesenchymal Transition ◽  
Quantitative Polymerase Chain Reaction ◽  
Immunohistochemical Analysis ◽  
Secretory Phase ◽  
Normal Endometrium ◽  
Mesenchymal Transition ◽  
Expression Levels ◽  
Endometrial Cells

Endometrial cells and microenvironment are two important factors in the pathogenesis of adenomyosis. Our previous study demonstrated that macrophages can induce eutopic epithelial cells of adenomyosis to suffer from epithelial–mesenchymal transition (EMT). The aim of this study is to detect whether macrophages interacting with epithelial cells equally induce the EMT process in normal and eutopic endometria of healthy and adenomyotic patients; and whether macrophages parallelly polarize to M2. We investigated the expression levels of epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), cytokeratin7 (CK7), vimentin, transforming growth factor-β1 (TGFB1), SMAD3 and pSMAD3 using immunohistochemistry and western blot, and then estimated the genetic levels of CD163, IL10 and MMP12 using real-time quantitative polymerase chain reaction (RT-PCR) in macrophages. Eutopic and normal endometrial tissues were obtained from 20 patients with adenomyosis and 11 control patients without adenomyosis, respectively. The immunohistochemical analysis shows distinct EMT in eutopic endometria in secretory phase; the expression levels of TGFB1, SMAD3 and pSMAD3 that indicate signal pathway of EMT were also higher in secretory phase. Macrophages can induce EMT process in primary endometrial epithelial cells derived from normal and eutopic endometria. After co-culturing, THP-1-derived macrophages polarized to M2. Compared with the eutopic endometrium group, further polarization to M2 was observed in the normal endometrium group. These results indicate that adenomyosis may be promoted by the pathologic EMT of epithelial cells, which is induced by macrophages that incapably polarize to M2.

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