An improved iliac lymph node method for production of monoclonal antibodies

2021 ◽  
Author(s):  
Tomoe Kobayashi ◽  
Masumi Namba ◽  
Mayumi Kohno ◽  
Takayuki Koyano ◽  
Yoshikazu Sado ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Lymph Node ◽  
Iliac Lymph Node

scholarly journals Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wenbo Jiang ◽  
Julius Wong ◽  
Hyon-Xhi Tan ◽  
Hannah G. Kelly ◽  
Paul G. Whitney ◽  
...  
Keyword(s):  
Animal Model ◽  
Monoclonal Antibodies ◽  
Lymph Node ◽  
Cross Reactivity ◽  
Tfh Cells ◽  
Follicular Helper ◽  
Lymph Node Cells ◽  
Human Viruses ◽  
Humoral Responses ◽  
Murine Monoclonal Antibodies

AbstractThe ferret is a key animal model for investigating the pathogenicity and transmissibility of important human viruses, and for the pre‐clinical assessment of vaccines. However, relatively little is known about the ferret immune system, due in part to a paucity of ferret‐reactive reagents. In particular, T follicular helper (Tfh) cells are critical in the generation of effective humoral responses in humans, mice and other animal models but to date it has not been possible to identify Tfh in ferrets. Here, we describe the screening and development of ferret-reactive BCL6, CXCR5 and PD-1 monoclonal antibodies. We found two commercial anti-BCL6 antibodies (clone K112-91 and clone IG191E/A8) had cross-reactivity with lymph node cells from influenza-infected ferrets. We next developed two murine monoclonal antibodies against ferret CXCR5 (clone feX5-C05) and PD-1 (clone fePD-CL1) using a single B cell PCR-based method. We were able to clearly identify Tfh cells in lymph nodes from influenza infected ferrets using these antibodies. The development of ferret Tfh marker antibodies and the identification of ferret Tfh cells will assist the evaluation of vaccine-induced Tfh responses in the ferret model and the design of novel vaccines against the infection of influenza and other viruses, including SARS-CoV2.

scholarly journals A Case of Canine Seminoma that Metastasized to a Medial Iliac Lymph Node and was Treated with Preoperative Irradiation and Surgical Resection

2013 ◽  
Vol 66 (8) ◽  
pp. 553-556
Author(s):  
Ryohei YAMAMOTO ◽  
Hideo AKIYOSHI ◽  
Hitomi HUKUI ◽  
Shogo HIRATA ◽  
Takeshi IZAWA ◽  
...  
Keyword(s):  
Lymph Node ◽  
Surgical Resection ◽  
Preoperative Irradiation ◽  
Iliac Lymph Node

scholarly journals Ascending colon cancer with synchronous right external iliac lymph node metastasis

2020 ◽  
Vol 9 (3) ◽  
pp. 162-167
Author(s):  
Yoshinori Iwata ◽  
Nobuhisa Matsuhashi ◽  
Takao Takahashi ◽  
Tomonari Suetsugu ◽  
Masahiro Fukada ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Ascending Colon ◽  
Node Metastasis ◽  
Iliac Lymph Node ◽  
Ascending Colon Cancer
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