PM 7/124 (1) Spodoptera littoralis, Spodoptera litura, Spodoptera frugiperda, Spodoptera eridania

Bracon hebetor is a Lepidopteran parasitoid which is used in IPM program as biological control agents. Corcyra cephalonica is fictitious host for mass rearing of B. hebetor in laboratory, rest hosts reared on their respective hosts i.e. Corcyra cephalonica, Plodia interpunctella, Ephestia kuehniella were reared on wheat flour, Galleria mellonella on its artificial diet, Helicoverpa armigera on chickpea pods, Earias vitella on okra fruits, Spodoptera litura and Spodoptera littoralis on castor leaves. The results of this study showed that the paralysis and parasitisation potential of B. hebetor were found higher on C. cephalonica followed by G. mellonella, E. kuehniella, P. interpunctella while it was shortest on S. litura and S. littoralis.

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Mate recognition and reproductive isolation in the sibling species Spodoptera littoralis and Spodoptera litura

Frontiers in Ecology and Evolution ◽

10.3389/fevo.2014.00018 ◽

2014 ◽

Vol 2 ◽

Cited By ~ 15

Author(s):

Ahmed M. Saveer ◽

Paul G. Becher ◽

GÃ¶ran Birgersson ◽

Bill S. Hansson ◽

Peter Witzgall ◽

...

Keyword(s):

Reproductive Isolation ◽

Sibling Species ◽

Spodoptera Littoralis ◽

Spodoptera Litura ◽

Mate Recognition

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Modification of Vip3Ab1 C-Terminus Confers Broadened Plant Protection from Lepidopteran Pests

Toxins ◽

10.3390/toxins11060316 ◽

2019 ◽

Vol 11 (6) ◽

pp. 316 ◽

Cited By ~ 2

Author(s):

Megan S. Sopko ◽

Kenneth E. Narva ◽

Andrew J. Bowling ◽

Heather E. Pence ◽

James J. Hasler ◽

...

Keyword(s):

Spodoptera Frugiperda ◽

Helicoverpa Zea ◽

Plant Protection ◽

Functional Expression ◽

In Planta ◽

Spodoptera Eridania ◽

Cry Proteins ◽

Lepidopteran Pests ◽

C Terminus ◽

Parent Protein

Vegetative insecticidal proteins (Vips) from Bacillus thuringiensis (Bt) are unique from crystal (Cry) proteins found in Bt parasporal inclusions as they are secreted during the bacterial vegetative growth phase and bind unique receptors to exert their insecticidal effects. We previously demonstrated that large modifications of the Vip3 C-terminus could redirect insecticidal spectrum but results in an unstable protein with no lethal activity. In the present work, we have generated a new Vip3 protein, Vip3Ab1-740, via modest modification of the Vip3Ab1 C-terminus. Vip3Ab1-740 is readily processed by midgut fluid enzymes and has lethal activity towards Spodoptera eridania, which is not observed with the Vip3Ab1 parent protein. Importantly, Vip3Ab1-740 does retain the lethal activity of Vip3Ab1 against other important lepidopteran pests. Furthermore, transgenic plants expressing Vip3Ab1-740 are protected against S. eridania, Spodoptera frugiperda, Helicoverpa zea, and Pseudoplusia includens. Thus, these studies demonstrate successful engineering of Vip3 proteins at the C-terminus to broaden insecticidal spectrum, which can be employed for functional expression in planta.

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Natural Enemies of Fall Armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) in Different Agro-Ecologies

Insects ◽

10.3390/insects12060509 ◽

2021 ◽

Vol 12 (6) ◽

pp. 509

Author(s):

Albert Fomumbod Abang ◽

Samuel Nanga Nanga ◽

Apollin Fotso Kuate ◽

Christiant Kouebou ◽

Christopher Suh ◽

...

Keyword(s):

Natural Enemies ◽

Spodoptera Frugiperda ◽

Development Time ◽

Fall Armyworm ◽

Spodoptera Eridania ◽

Southern Armyworm ◽

Parasitism Rates ◽

Emergency Actions ◽

Indigenous Natural Enemies ◽

Telenomus Remus

Fall armyworm (FAW) Spodoptera frugiperda (J.E. Smith) and southern armyworm (SAW) Spodoptera eridania (Stoll) have become major threats to crops in Africa since 2016. African governments adopted emergency actions around chemical insecticides, with limited efforts to assess the richness or roles of indigenous natural enemies. Field surveys and laboratory studies were conducted to identify and assess the performance of parasitoids associated with spodopterans in Cameroon. FAW was the most abundant spodopteran pest. Telenomus remus (Nixon), Trichogramma chilonis (Ishi), Charops sp. (Szépligeti), Coccygidium luteum (Cameron), Cotesia icipe (Fernandez & Fiaboe), and Cotesia sesamiae (Cameron) are the first records in the country on spodopterans. Telenomus remus, T. chilonis, C. icipe, and Charops sp. were obtained from both FAW and SAW; C. luteum and C. sesamiae from FAW. The distribution of spodopterans, their endoparasitoids, and parasitism rates varied with host, season and location. In the laboratory, T. remus showed significantly higher parasitism on FAW than SAW, and significant differences in the development parameters between the two host eggs, with shorter development time on FAW. It induced significant non-reproductive mortality on FAW but not on SAW. Developmental parameters showed that C. icipe has a shorter development time compared to other larval parasitoids. Implications for conservative and augmentative biocontrol are discussed.

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Nomuraea rileyi (Farlow) Samson Incidence on Spodoptera litura and Spodoptera frugiperda in Andhra Pradesh

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2021.1002.149 ◽

2021 ◽

Vol 10 (2) ◽

pp. 1262-1270

Author(s):

Y. Peeru Saheb K. Manjula ◽

K. Devaki R. Sarada Jaya Lakshmi ◽

B. Ravindra Reddy N. C. Venkateswarlu

Keyword(s):

Spodoptera Frugiperda ◽

Spodoptera Litura ◽

Andhra Pradesh ◽

Nomuraea Rileyi

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Avidin expression does not increase speed of kill of Spodoptera litura by baculovirus

New Zealand Plant Protection ◽

10.30843/nzpp.2003.56.6042 ◽

2003 ◽

Vol 56 ◽

pp. 194-200 ◽

Cited By ~ 1

Author(s):

L.N. Gatehouse ◽

N.P. Markwick ◽

J. Poulton ◽

J.T. Christeller

Keyword(s):

Spodoptera Frugiperda ◽

Spodoptera Litura ◽

Culture Medium ◽

Fluorescent Protein ◽

Insect Cell Culture ◽

Autographa Californica ◽

Green Fluorescence ◽

Strong Fluorescence ◽

Nuclear Polyhedrosis ◽

Green Fluorescent

A disarmed (polhminus) Autographa californica nuclear polyhedrosis virusbased baculovirus expressing green fluorescent protein (GFP) on the strong late promotor p10 was constructed A second virus was made which also expressed the biotinbinding protein avidin from the strong late promotor polh Both viruses caused strong fluorescence in Spodoptera frugiperda cells in insect cell culture Sevendayold larvae of Spodoptera litura were inoculated by microinjection with GFP virus GFPavidin virus or tissue culture medium (control) Mortality growth rate and the development of green fluorescence were followed and after death the larvae were analysed for GFP and avidin content All virusinjected larvae developed strong fluorescence However no significant differences in growth or mortality were seen between the larvae injected with virus expressing and not expressing avidin Both GFP and avidin accumulated to high levels in the larvae The expression of avidin did not change the level of expression of GFP

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Optimizing Photoperiod, Exposure Time, and Host-to-Parasitoid Ratio for Mass-Rearing of Telenomus remus, an Egg Parasitoid of Spodoptera frugiperda, on Spodoptera litura Eggs

Insects ◽

10.3390/insects12121050 ◽

2021 ◽

Vol 12 (12) ◽

pp. 1050

Author(s):

Wanbin Chen ◽

Qingfen Weng ◽

Rui Nie ◽

Hongzhi Zhang ◽

Xiaoyu Jing ◽

...

Keyword(s):

Exposure Time ◽

Spodoptera Frugiperda ◽

Spodoptera Litura ◽

Negative Relationship ◽

Significant Negative Correlation ◽

Mass Rearing ◽

Egg Parasitoid ◽

Female Progeny ◽

Effective Manner ◽

Telenomus Remus

Telenomus remus (Nixon) is a dominant egg parasitoid of the destructive agricultural pest Spodoptera frugiperda (J. E. Smith), and so is used in augmentative biocontrol programs in several countries. An optimized mass-rearing system is essential to produce biological control products in a timely and cost-effective manner. In this study, the photoperiod, host egg:parasitoid ratio, and exposure time were evaluated to identify the optimal rearing conditions for T. remus on the alternative host Spodoptera litura (Fabricius) eggs. Results showed that increasing photoperiod above 12L:12D remarkably improved parasitoid progeny yield and life table parameters. Overlong photoperiods shortened female longevity, but within acceptable limits. There was a significant negative correlation between parasitism rate and host egg:parasitoid ratio under exposure times of 12 and 36 h, but not 24 h. Percentage of female progeny increased significantly along with increasing the host egg:parasitoid ratio. A significant negative relationship between the number of emerged adults per egg and the host egg:parasitoid ratio was observed at an exposure time of 36 h. It was concluded that T. remus may be mass-reared most efficiently on S. litura eggs using a photoperiod of more than 12L:12D, a 14–20:1 host egg:parasitoid ratio, and an exposure time of 24 h. These findings can be used to produce T. remus more efficiently and at lower costs.

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