Lymphocyte Subpopulations in Chronic Lymphocytic Leukemia (CLL)

We have defined two subpopulations of B lymphocytes in chronic lymphocytic leukemia (CLL). The major variant (termed typical) was characterized by the presence of a relatively homogeneous population of small-to-medium-sized lymphocytes with low-density SmIgM and no evidence of intracellular Ig synthesis. A minor group (termed atypical) was identified by the presence of a pleomorphic cell population with few small lymphocytes. The predominant SmIg was IgG, which was detected intracellularly as well as in the serum. The atypical group appeared to be an arrest at a later stage of differentiation where a switch from IgM to IgG synthesis and secretion had occurred. Clinical correlation suggested several clear-cut differences in clinicopathologic characteristics, but the median survivals for both groups at 2 yr was nearly identical.

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T-lymphocyte subpopulations in chronic lymphocytic leukemia: A quantitative and functional study

Cancer ◽

10.1002/1097-0142(19811115)48:10<2191::aid-cncr2820481013>3.0.co;2-9 ◽

1981 ◽

Vol 48 (10) ◽

pp. 2191-2197 ◽

Cited By ~ 27

Author(s):

G. Semenzato ◽

A. Pezzutto ◽

C. Agostini ◽

M. Albertin ◽

G. Gasparotto

Keyword(s):

Chronic Lymphocytic Leukemia ◽

T Lymphocyte ◽

Lymphocyte Subpopulations ◽

Lymphocytic Leukemia ◽

Functional Study ◽

T Lymphocyte Subpopulations

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Lactic dehydrogenase in normal and leukemia lymphocyte subpopulations: evidence for the presence of abnormal T cells and B cells in chronic lymphocytic leukemia

Blood ◽

10.1182/blood.v57.2.324.324 ◽

1981 ◽

Vol 57 (2) ◽

pp. 324-327 ◽

Cited By ~ 14

Author(s):

P Rambotti ◽

S Davis

Keyword(s):

T Cells ◽

B Cells ◽

Chronic Lymphocytic Leukemia ◽

T Lymphocytes ◽

B Lymphocytes ◽

Lactic Dehydrogenase ◽

Isozyme Pattern ◽

Lymphocyte Subpopulations ◽

Lymphocytic Leukemia ◽

Ldh Activity

Abstract Lactic dehydrogenase (LDH) was quantitated and the isozyme pattern studied in lymphocyte subpopulations from normal people and patients with chronic lymphocytic leukemia (CLL). Normal T lymphocytes differed from normal B lymphocytes in having greater total LDH activity (597.2 versus 252.1). Total LDH activity in CLL T cells (347.1) was lower than normal T cells., but not significantly different than normal B cells. Total LDH activity in CLL B cells (124.6) was lower then normal B cells and normal T cells. The isozyme pattern of normal T lymphocytes showed a higher activity in the LDH-1 band (26.7% versus 5.4%) but showed a lower activity in LDH-5 band (4.3% versus 16.3%) compared to normal B cells. Chronic lymphocytic leukemia T cells could be distinguished from CLL B cells by a high LDH-5 band (22.3% versus 7.6%) and from normal T cells by a high LDH-5 band (22.3% versus 4.3%) and a low LDH-1 band (7.3% versus 26.7%). CLL B cells could be distinguished from normal B cells by a low LDH-5 band (7.6% versus 16.3%). Thus, the LDH isozyme pattern distinguishes normal T lymphocytes from normal B lymphocytes, and normal T and B lymphocytes from CLL T and B lymphocytes.

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B lymphocyte subpopulations in chronic lymphocytic leukemia

Blood ◽

10.1182/blood.v47.2.229.229 ◽

1976 ◽

Vol 47 (2) ◽

pp. 229-235 ◽

Cited By ~ 23

Author(s):

RA Rudders

Keyword(s):

Chronic Lymphocytic Leukemia ◽

B Lymphocyte ◽

Lymphocyte Subpopulations ◽

Lymphocytic Leukemia ◽

Clinicopathologic Characteristics ◽

Homogeneous Population ◽

Clear Cut ◽

Igg Synthesis ◽

A Minor ◽

Ig Synthesis

Abstract We have defined two subpopulations of B lymphocytes in chronic lymphocytic leukemia (CLL). The major variant (termed typical) was characterized by the presence of a relatively homogeneous population of small-to-medium-sized lymphocytes with low-density SmIgM and no evidence of intracellular Ig synthesis. A minor group (termed atypical) was identified by the presence of a pleomorphic cell population with few small lymphocytes. The predominant SmIg was IgG, which was detected intracellularly as well as in the serum. The atypical group appeared to be an arrest at a later stage of differentiation where a switch from IgM to IgG synthesis and secretion had occurred. Clinical correlation suggested several clear-cut differences in clinicopathologic characteristics, but the median survivals for both groups at 2 yr was nearly identical.

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