The tissue localization of Aeromonas salmonicida in rainbow trout, Salmo gairdneri Richardson, following three methods of administration

1984 ◽  
Vol 25 (1) ◽  
pp. 95-108 ◽  
Author(s):  
M. F. Tatner ◽  
C. M. Johnson ◽  
M. T. Horne
Keyword(s):  
Rainbow Trout ◽  
Aeromonas Salmonicida ◽  
Salmo Gairdneri ◽  
Tissue Localization

Aeromonas salmonicida: Relationship between Extracellular Growth Products and Isolate Virulence

1981 ◽  
Vol 38 (11) ◽  
pp. 1322-1326 ◽  
Author(s):  
Rocco C. Cipriano ◽  
Billy R. Griffin ◽  
Burton C. Lidgerding
Keyword(s):  
Rainbow Trout ◽  
Brook Trout ◽  
Salvelinus Fontinalis ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Aeromonas Salmonicida ◽  
Salmo Gairdneri ◽  
Lesion Development ◽  
Culture Supernatants ◽  
Crude Material

Extracellular growth products, extracted from culture supernatants of Aeromonas salmonicida by precipitation with ammonium sulfate and ethanol, were resolved into four fractions by ion-exchange chromatography. Although one of these (fraction II) possessed leukocytolytic activity, virulence of the pathogen was not associated with leukocytotoxicity. A positive correlation was established, however, between virulence and the toxicity of extracted material to cultured rainbow trout gonad (RTG-2) cells. The crude material and fractions II and III from virulent isolates were more toxic to RTG-2 cells than were similar preparations from avirulent isolates. Preparations from virulent isolates caused hemorrhaging, lesion development, and mortality when injected intramuscularly into brook trout (Salvelinus fontinalis) and Atlantic salmon (Salmo solar). Rainbow trout (Salmo gairdneri) were more resistant and lesions could be produced only with fraction II. Fraction II also possessed proteolytic activity.Key words: Aeromonas salmonicida, furunculosis, pathogenicity, salmonids, extracellular products, cytotoxicity, leukocytolysis, proteolysis, fishes

A Standardized Model of Experimental Furunculosis in Rainbow Trout (Salmo gairdneri)

1980 ◽  
Vol 37 (4) ◽  
pp. 746-750 ◽  
Author(s):  
C. Michel
Keyword(s):  
Rainbow Trout ◽  
Intramuscular Injection ◽  
Infected Fish ◽  
Aeromonas Salmonicida ◽  
Salmo Gairdneri ◽  
Exponential Growth Phase ◽  
Cell Counts ◽  
Viable Cells ◽  
Treated Fish ◽  
Drop Plate

With the aid of published information, we have developed a standardized and reproducible experimental model of furunculosis in rainbow trout (Salmo gairdneri). The infective doses (LD50 = 200 to 2000 germs, i.m.) employed strains of Aeromonas salmonicida, the virulence of which was maintained by passage in 15-g fingerlings. The number of viable cells in the dose was conveniently determined using the drop-plate enumeration technique; however, meaningful cell counts could only be obtained if broth cultures used for infecting the fish were harvested early in the exponential growth phase (OD < 1.000 at 525 nm). Better results were obtained with intramuscular injection than with intraperitoneal injection. The infection procedure involved injecting a dose of 10 LD50, intramuscularly, into each of 30 fish held at 15 °C and recording the mortalities for 10 d. Protection tests in which Tribrissen (28 mg sulfadiazine and 5.6 mg trimethoprim/kg fish for 8 d) was fed or tetracycline (1 mg per fish) i.m. injected into the infected fish served to demonstrate the value of the model. Results were in agreement with field observations with no death for treated fish and a mortality of 96%, for untreated fish. Key wordss rainbow trout, Salmo gairdneri; Aeromonas salmonicida, furunculosis, experimental infection

A Leukocytolytic Factor Isolated from Cultures of Aeromonas salmonicida

1977 ◽  
Vol 34 (8) ◽  
pp. 1118-1125 ◽  
Author(s):  
D. W. Fuller ◽  
K. S. Pilcher ◽  
J. L. Fryer
Keyword(s):  
Rainbow Trout ◽  
Virulence Factors ◽  
Coho Salmon ◽  
Virulent Strain ◽  
Deae Cellulose ◽  
Aeromonas Salmonicida ◽  
Salmo Gairdneri ◽  
Amino Sugars ◽  
Avirulent Strain

A substance characterized as a glycoprotein, isolated from the supernatant fluids of broth cultures of Aeromonas salmonicida by a combination of ammonium sulfate and ethanol precipitations followed by chromatography on DEAE-cellulose, was cytolytic for rainbow trout (Salmo gairdneri) leukocytes, and antigenic when injected into rabbits. The ratio of protein to hexose determined by analysis of the purified fraction was between 0.35 and 0.45, and small amounts of amino sugars were detected. A virulent strain of A. salmonicida produced much more of this factor than an avirulent strain. This factor was cytolytic for leukocytes in vitro and also produced a pronounced leukopenia when injected intravenously in adult rainbow trout. When injected in small coho salmon (Oncorhyncus kisutch) 8–13 cm long together with about one LD50 of live A. salmonicida 36 of 40 fish succumbed to the combination, whereas only 14 of 40 died from an injection of the bacterium alone. Thus, the pathogenicity of the organism was enhanced, presumably by increasing the susceptibility of the host. Hence, this glycoprotein apparently is one of the virulence factors of this bacterium. Key words: leukocytolytic factor, Aeromonas salmonicida, glycoprotein, virulence factor

Precipitating Antibody Against Aeromonas salmonicida in Serums of Inbred Albino Rainbow Trout (Salmo gairdneri)

1970 ◽  
Vol 27 (8) ◽  
pp. 1389-1393 ◽  
Author(s):  
Douglas P. Anderson ◽  
George W. Klontz
Keyword(s):  
Rainbow Trout ◽  
Gamma Globulin ◽  
Aeromonas Salmonicida ◽  
Salmo Gairdneri ◽  
Rabbit Serum ◽  
Antibody Activity ◽  
Parenteral Exposure ◽  
Beta 2 ◽  
Gel Diffusion

Precipitins in albino rainbow trout serums were demonstrated by gel diffusion after a single parenteral exposure to the soluble antigens of Aeromonas salmonicida. The fraction of the serum containing antibody activity against the presented antigens was shown by immunoelectrophoresis to be in the nonmigrating region. This corresponded to the beta-2 fraction of rabbit serum. An antibody-containing component comparable with rabbit gamma globulin was not detected.

Pathophysiology of Infectious Hematopoietic Necrosis Virus Disease in Rainbow Trout (Salmo gairdneri): Early Changes in Blood and Aspects of the Immune Response after Injection of IHN Virus

1974 ◽  
Vol 31 (8) ◽  
pp. 1371-1378 ◽  
Author(s):  
Donald F. Amend ◽  
Lynnwood Smith
Keyword(s):  
Immune Response ◽  
Rainbow Trout ◽  
Blood Cell ◽  
Juvenile Fish ◽  
Passive Immunization ◽  
Aeromonas Salmonicida ◽  
Salmo Gairdneri ◽  
Necrosis Virus ◽  
Acid Base ◽  
Infectious Hematopoietic Necrosis

Juvenile rainbow trout (Salmo gairdneri) were injected with infectious hematopoietic necrosis (IHN) virus and various hematological and blood chemical changes were monitored over 9 days. The packed cell volume, hemoglobin, red blood cell count, and plasma bicarbonate were significantly depressed by day 4. Plasma chloride, calcium, phosphorus, total protein, and blood cell types did not change during the 9 days. Furthermore, plasma [Formula: see text] LDH isozyme was significantly increased by the fourth day, and fish infected with infectious pancreatic necrosis virus, Vibrio anguillarum, Aeromonas salmonicida, and redmouth bacterium did not show specific LDH isozyme alterations. Acid-base alterations occurred at 10 C but not at 18 C. The acid-base imbalance and elevation of the [Formula: see text] LDH isozyme were consistently associated with the early development of the disease.The immune response after injection of IHN virus was determined and protection from disease was tested by passive immunization. Actively immunized fish developed IHN-neutralizing antibodies within 54 days after injection of virus, and the antibodies were protective when juvenile fish were passively immunized and experimentally challenged with IHN virus.

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