Hypervariable minisatellite DNA single locus probes for the Atlantic salmon, Salmo salar L.

1990 ◽  
Vol 37 (6) ◽  
pp. 991-993 ◽  
Author(s):  
J. B. Taggart ◽  
A. Ferguson
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Single Locus ◽  
Minisatellite Dna ◽  
Single Locus Probes ◽  
Atlantic Salmon Salmo Salar

A minisatellite DNA marker for discriminating between European and North American Atlantic salmon (Salmo salar)

1995 ◽  
Vol 52 (11) ◽  
pp. 2305-2311 ◽  
Author(s):  
John B. Taggart ◽  
Eric Verspoor ◽  
Paul T. Galvin ◽  
Paloma Morán ◽  
Andrew Ferguson
Keyword(s):  
Atlantic Salmon ◽  
Allele Frequency ◽  
Salmo Salar ◽  
North American ◽  
Nuclear Dna ◽  
Dna Marker ◽  
Low Frequency ◽  
Geographic Range ◽  
Minisatellite Dna ◽  
Atlantic Salmon Salmo Salar

A highly discriminatory and practical nuclear DNA genetic marker that can distinguish between Atlantic salmon (Salmo salar) of European and North American origin is described. Screening of 2847 European and 247 North American Atlantic salmon from much of its geographic range for variability at a minisatellite locus, Ssa-A45/2/2, revealed the continental stocks to be almost fixed for two different-sized, easily discernable alleles. Virtually all European Atlantic salmon were homozygous for a 3.00-kb allele (frequency > 0.999), while a smaller 2.77-kb allele (frequency = 0.946) predominated in all North American populations. Whereas the 2.77-kb allele was found exclusively in North American salmon, an allele indistinguishable in size from the 3.00-kb European diagnostic allele was also observed at low frequency (0.036) in North American fish. Eight other continent-specific rare alleles (highest frequency = 0.006) were also observed. The results suggest that little, if any, natural gene flow occurs between the two continental groups of Atlantic salmon.

Comparison, using minisatellite DNA profiling, of secondary male contribution in the fertilisation of wild and ranched Atlantic salmon (Salmo salar) ova

1998 ◽  
Vol 55 (9) ◽  
pp. 2011-2018 ◽  
Author(s):  
C E Thompson ◽  
W R Poole ◽  
M A Matthews ◽  
A Ferguson
Keyword(s):  
Reproductive Success ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Minisatellite Dna ◽  
Atlantic Salmon Salmo Salar ◽  
The Difference ◽  
Western Ireland ◽  
Minimum Numbers ◽  
Representative Samples ◽  
Male Genotype

Secondary males, either subdominant adults or, more usually, parr which mature in freshwater, are known to successfully fertilise Atlantic salmon (Salmo salar) ova. This study has estimated the reproductive success of such secondary males in redds formed by pairs of wild and sea-ranched adults. Eight wild and 11 ranched redds were sampled from the Burrishoole system, western Ireland, in two consecutive years. Representative samples of fertilised ova were screened at three minisatellite loci to reconstruct the genotypes of putative anadromous parents and to estimate the proportion of eggs fertilised by secondary males. Multiple paternal genotypes were detected in 18 of the 19 samples, and of the 1484 progeny examined, 593 could not have been derived from the primary adult male genotype. The level of secondary male contribution detected among wild redds from Burrishoole (mean 28.9%) was similar to that found in previous published studies. The level among ranched redds was significantly higher (mean 48.2%) (P = 0.042). The difference was greater when data from 1995 were considered alone, with the average level of secondary male contribution in ranched redds being almost twice that in wild redds. Minimum numbers of secondary males were not significantly different between wild (mean 2.6) and ranched (mean 2.7) redds.

Microstructure and texture of fresh and smoked Atlantic salmon, Salmo salar L., fillets from fish reared and slaughtered under different conditions

2001 ◽  
Vol 32 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Sjofn Sigurgisladottir ◽  
Margret S. Sigurdardottir ◽  
Helga Ingvarsdottir ◽  
Ole J. Torrissen ◽  
Hannes Hafsteinsson
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Atlantic Salmon Salmo Salar

Persistence of Orally Administered Salmonella enterica Serovars Agona and Montevideo in Atlantic Salmon (Salmo salar L.)

2005 ◽  
Vol 68 (7) ◽  
pp. 1336-1339 ◽  
Author(s):  
L. L. NESSE ◽  
T. LØVOLD ◽  
B. BERGSJØ ◽  
K. NORDBY ◽  
C. WALLACE ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Salmonella Enterica ◽  
Most Probable Number ◽  
Fish Feed ◽  
Internal Organs ◽  
Probable Number ◽  
Atlantic Salmon Salmo Salar ◽  
Gastric Intubation

The objective of our experiments was to study the persistence and dissemination of orally administered Salmonella in smoltified Atlantic salmon. In experiment 1, salmon kept at 15°C were fed for 1 week with feed contaminated with 96 most-probable-number units of Salmonella Agona per 100 g of feed and then starved for 2 weeks. Samples were taken from the gastrointestinal tract and examined for Salmonella 1, 2, 8, 9, 15, and 16 days after the feeding ended. In experiment 2, Salmonella Agona and Montevideo were separately mixed with feed and administered by gastric intubation. Each fish received 1.0 × 108, 1.0 × 106, or 1.0 × 104 CFU. The different groups were kept in parallel at 5 and 15°C and observed for 4 weeks. Every week, three fish in each group were sacrificed, and samples were taken from the skin, the pooled internal organs, the muscle, and the gastrointestinal tract and examined for the presence of Salmonella. The results from the two experiments showed that the persistence of Salmonella in the fish was highly dependent on the dose administered. Salmonella was not recovered from any of the fish that were fed for 1 week with the lowest concentration of Salmonella. In the fish given the highest dose of Salmonella, bacteria persisted for at least 4 weeks in the gastrointestinal tract as well as, to some extent, the internal organs. The present study shows that under practical conditions in Norway, the risk of Salmonella in fish feed being passed on to the consumer of the fish is negligible.

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