ANTI-DNA ANTIBODIES OF VARIOUS SKIN DISEASES, ESPECIALLY OF SYSTEMIC LUPUS ERYTHEMATOSUS

Objective. Systemic lupus erythematosus (SLE) is an autoimmune disease identified by a plethora of production of autoantibodies. Autoreactive T cells may play an important role in the process. Attenuated T cell vaccination (TCV) has proven to benefit some autoimmune diseases by deleting or suppressing pathogenic T cells. However, clinical evidence for TCV in SLE is still limited. Therefore, this self-controlled study concentrates on the clinical effects of TCV on SLE patients. Methods. 16 patients were enrolled in the study; they accepted TCV regularly. SLEDAI, clinical symptoms, blood parameters including complements 3 and 4 levels, ANA, and anti-ds-DNA antibodies were tested. In addition, the side effects and drug usage were observed during the patients’ treatment and follow-up. Results. Remissions in clinical symptoms such as facial rash, vasculitis, and proteinuria were noted in most patients. There are also evident reductions in SLEDAI, anti-ds-DNA antibodies, and GC dose and increases in C3 and C4 levels, with no pathogenic side effects during treatment and follow-up. Conclusions. T cell vaccination is helpful in alleviating and regulating systemic lupus erythematosus manifestation.

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ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS ERYTHEMATOSUS

Rheumatic Disease Clinics of North America ◽

10.1016/s0889-857x(21)00736-5 ◽

1992 ◽

Vol 18 (2) ◽

pp. 437-454

Author(s):

David S. Pisetsky

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Systemic Lupus ◽

Dna Antibodies

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Immunological and Clinical Characteristics of Systemic Lupus Erythematosus: A Series from Morocco

BioMed Research International ◽

10.1155/2018/3139404 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Zeineb Zian ◽

Mouna Maamar ◽

Mohamed El Aouni ◽

Amina Barakat ◽

Naima Ghailani Nourouti ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Clinical Manifestations ◽

Arab Countries ◽

University Hospital ◽

Nuclear Antigen ◽

Systemic Lupus ◽

Female Predominance ◽

Dna Antibodies ◽

Moroccan Patients

Systemic Lupus Erythematosus (SLE) is a complex autoimmune disease with a high female predominance. To date, studies about SLE in Morocco are few. This retrospective study describes the clinical and immunological features in a series of 50 SLE Moroccan patients in University Hospital Center of Rabat, Morocco, between December 2011 and December 2013. All patients were screened for antinuclear antibodies (ANA) and anti-DNA antibodies by indirect immunofluorescence, followed by identification of anti-extractable nuclear antigen antibodies by ELISA. The female to male ratio was 6.1:1. Mean age was 31.72 years. The main clinical manifestations were arthritis (82%), mucocutaneous manifestations (80%), renal manifestations (50%), and hematological features (46%). Of the mucocutaneous features, the highest frequencies were observed in the malar rash (68%) and photosensitivity (60%). Of the hematological features, lymphopenia was most frequently observed in 30% of patients, followed by hemolytic anemia in 16% and leucopenia and thrombocytopenia in 8%. Central nervous system was involved in 10%. ANA were found in 88%, anti-DNA antibodies in 56%, and anti-Sm antibodies in 50%. Anti-SSA, anti-SSB, anti-Sm/RNP, and anti-Scl70 antibodies were detected in 38%, 10%, 48%, and 8%, respectively. Our data show that, in our patients, the main clinical and immunological features of SLE remain comparable to patients from other Arab countries.

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Differential immunoadsorption coupled with rate nephelometry for estimation of DNA-binding immunoglobulins.

Clinical Chemistry ◽

10.1093/clinchem/30.7.1187 ◽

1984 ◽

Vol 30 (7) ◽

pp. 1187-1192 ◽

Cited By ~ 3

Author(s):

V A DeBari ◽

J Nicotra ◽

J F Blaney ◽

E F Schultz ◽

M A Needle

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Binding Assay ◽

Systemic Lupus ◽

Control Serum ◽

Present Technique ◽

Before And After ◽

Dna Antibodies ◽

Nonparametric Correlation ◽

Combined Data

Abstract We describe a technique for estimating the mass of anti-DNA antibodies by immunonephelometry of serum immunoglobulins (IgG, IgA, IgM) before and after adsorption onto DNA bound to agarose-polylysine columns. Sixteen patients with systemic lupus erythematosus and 16 age- and sex-matched controls were studied. Precision was determined for high-value (in 10 patients) and low-value (in nine controls) ranges for each of the immunoglobulins. Within-run CVs ranged from 3.0% (IgG, controls) to 11.8% (IgA, patients); between-run CVs ranged from 15.5% (IgG, patients) to 25.2% (IgM, patients). We found anti-DNA antibody concentrations (mean +/- SD) in systemic lupus erythematosus of 1.981 +/- 1.015 g/L for IgG (controls: 0.243 +/- 0.231, p less than 0.001), 0.257 +/- 0.215 g/L for IgA (controls: 0.038 +/- 0.035, p less than 0.001), and 0.282 +/- 0.234 g/L for IgM (controls: 0.191 +/- 0.165, p greater than 0.05). Sensitivity and linearity are such that fivefold dilutions of patients' serum with either a buffered albumin solution or control serum yielded values close to the expected values for IgG. Similarly diluted sera gave inordinately high values in the radiometric binding assay. Neither parametric (linear regression) nor nonparametric correlation methods (Spearman's rank and Kendall's tau) show a significant correlation between patients' data obtained by the present technique and that by a radiometric binding assay (p greater than 0.05), although combined data from patients and controls demonstrate a significant nonparametric correlation (p less than 0.005 for Spearman's and p less than 0.02 for Kendall's).

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Monoclonal human anti-DNA antibodies from EB virus-transformed lymphocytes of systemic lupus erythematosus (SLE) patients

Journal of Clinical Immunology ◽

10.1007/bf00929459 ◽

1985 ◽

Vol 5 (4) ◽

pp. 246-253 ◽

Cited By ~ 20

Author(s):

Takeshi Sasaki ◽

Tai Muryoi ◽

Yukio Sekiguchi ◽

Eiichi Tamate ◽

Kaoru Yoshinaga ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Systemic Lupus ◽

Eb Virus ◽

Dna Antibodies

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Basophil Recruitment to Skin Lesions of Patients with Systemic Lupus Erythematosus Mediated by CCR1 and CCR2

Cellular Physiology and Biochemistry ◽

10.1159/000481609 ◽

2017 ◽

Vol 43 (2) ◽

pp. 832-839 ◽

Cited By ~ 14

Author(s):

Qingjun Pan ◽

Yongmin Feng ◽

Yanxia Peng ◽

Hongjiu Zhou ◽

Zhenzhen Deng ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Migration Rate ◽

Skin Diseases ◽

Normal Skin ◽

Flow Cytometric Analysis ◽

Skin Lesions ◽

Skin Tissue ◽

Systemic Lupus

Background/Aims: Basophils have been reported to infiltrate skin lesions in various skin diseases, but not in systemic lupus erythematosus (SLE). This study investigated basophil infiltration in SLE and its mechanism. Methods: Twenty newly diagnosed SLE patients and twenty healthy controls were enrolled. Nine SLE patients underwent skin biopsies. Flow cytometric analysis the phenotype of peripheral basophils and their migration rate toward RANTES and MCP-1 were analyzed with the transwell culture system, also the expression of these two chemokines in skin tissue were analyzed with immunohistochemistry. Results: Increased activation and decreased numbers of peripheral basophils were observed in SLE patients compared with controls. Basophil migration into skin lesions of SLE patients were observed, but not in normal skin tissue. This migration was related to the upregulation of chemokine receptors CCR1 and CCR2 on basophils. In vitro studies showed that migration rate toward RANTES and MCP-1 increased significantly in basophils from SLE patients compared with those from controls. Consistently, high levels of RANTES and MCP-1 expression were observed in skin lesions from SLE patients but not in normal skin tissue. Conclusion: Basophil recruitment to skin lesions of SLE patients mediated by CCR1 and CCR2, which may contribute to tissue damage in SLE.

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