scholarly journals A Mouse Erythroleukemia Cell Line Possessing Friend Spleen Focus-forming Virus gp55 Transgene and Temperature-sensitive Mutant p53 Gene

1995 ◽  
Vol 86 (3) ◽  
pp. 284-291 ◽  
Author(s):  
Xin Xu ◽  
Yasuko Yamamura ◽  
Teruyo Tsukada ◽  
Mitsuaki A. Yoshida ◽  
Hisato Senda ◽  
...  
Keyword(s):  
Cell Line ◽  
P53 Gene ◽  
Temperature Sensitive ◽  
Mutant P53 ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Erythroleukemia Cell ◽  
Mouse Erythroleukemia

Molecular cloning of the human gene, CCG2, that complements the BHK-derived temperature-sensitive cell cycle mutant tsBN63: identity of CCG2 with the human X chromosomal SCAR/RPS4X gene

1991 ◽  
Vol 100 (1) ◽  
pp. 35-43
Author(s):  
M. Watanabe ◽  
N. Furuno ◽  
M. Goebl ◽  
M. Go ◽  
K. Miyauchi ◽  
...  
Keyword(s):  
Cell Line ◽  
Genomic Dna ◽  
Human Gene ◽  
G1 Phase ◽  
Temperature Sensitive ◽  
Nonpermissive Temperature ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
G2 Phase ◽  
Synchronized Cultures

A temperature-sensitive mutant tsBN63 cell line was isolated by the fluorodeoxyuridine method from the BHK21/13 cell line after mutagenesis with nitrosoguanidine. When cultures of tsBN63 cells growing asynchronously at 33.5 degrees C were shifted to 39.5 degrees C, a nonpermissive temperature, the ability for protein synthesis was rapidly reduced and cell proliferation stopped mainly at G1 phase, and partly at G2 phase. Synchronized cultures of tsBN63 cells did not commence DNA synthesis when shifted up in G1 phase. The human gene complementing the tsBN63 mutation was cloned by DNA-mediated gene transfer and its cDNA of 1.1 kb conferring ts+ phenotype on tsBN63 cells was isolated from the cDNA library of Raj (mer+) cells with a frequency of 10(−3). On the basis of the determined nucleotide sequence, the isolated human gene turned out to be the X chromosomal RPS4X encoding the ribosomal protein S4. The size of the CCG2 gene was estimated to be about 12 kb by complementation analysis of the tsBN63 mutation with cloned genomic DNA.

Establishment and characteristics of a practical and useful astrocyte cell line transformed by a temperature-sensitive mutant of simian virus 40

1997 ◽  
Vol 759 (2) ◽  
pp. 295-300 ◽  
Author(s):  
Yukisato Kitamura ◽  
Makoto Michikawa ◽  
Takahiko Tanigawa ◽  
Tetsuo Katsumoto ◽  
Takehito Morita ◽  
...  
Keyword(s):  
Cell Line ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant

Rescue of aDrosophila temperature-sensitive mutant cell line by DNA transfection

1984 ◽  
Vol 10 (6) ◽  
pp. 573-577 ◽  
Author(s):  
J. H. Sang ◽  
J. H. Sinclair ◽  
J. F. Burke ◽  
D. Ish-Horowicz
Keyword(s):  
Cell Line ◽  
Mutant Cell ◽  
Temperature Sensitive ◽  
Dna Transfection ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Mutant Cell Line
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