Polymerase chain reaction analysis of mitochondrial DNA polymorphism in N'Dama and Zebu cattle

2009 ◽  
Vol 24 (5) ◽  
pp. 339-343 ◽  
Author(s):  
R. Suzuki ◽  
S. J. Kemp ◽  
A. J. Teale
Keyword(s):  
Polymerase Chain Reaction ◽  
Mitochondrial Dna ◽  
Dna Polymorphism ◽  
Polymerase Chain Reaction Analysis ◽  
Zebu Cattle ◽  
Chain Reaction ◽  
Mitochondrial Dna Polymorphism ◽  
Polymerase Chain

Detection of cows' milk in goats' cheeses inferred from mitochondrial DNA polymorphism

2001 ◽  
Vol 68 (2) ◽  
pp. 229-235 ◽  
Author(s):  
CÉLIA MAUDET ◽  
PIERRE TABERLET
Keyword(s):  
Mitochondrial Dna ◽  
Dna Polymorphism ◽  
Simple Approach ◽  
Milk Analysis ◽  
Chain Reaction ◽  
Specific Primers ◽  
Mitochondrial Dna Polymorphism ◽  
Sequence Variations ◽  
Spin Column ◽  
Polymerase Chain

A new polymerase chain reaction (PCR)-based method was developed to detect cows' milk in goat cheese. This method is based on mitochondrial DNA (mtDNA) control region sequence variations. DNA extractions from 150 mg of cheese were carried out using a spin column-based method. Subsequent PCR amplifications of DNA were performed with cow specific primers, demonstrating the ability to detect cows' milk in a variety of cheeses. This simple approach provides high quality DNA, and is shown to be very sensitive, with a detection limit of less than 0·1% of cows' milk. Analysis of an agarose gel digital image allows a rough estimation of the percentage of cows' milk used in adulteration.

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