Association of MT-ND5 gene variation with mitochondrial respiratory control ratio and NADH dehydrogenase activity in Tibet chicken embryos

PURPOSE: To analyze the effects of hyperbaric oxygen therapy on liver function in rats previously subjected to ischemia and reperfusion. METHODS: A randomly distribution of 23 Wistar rats was conducted into three groups: SHAM, animals subjected to surgical stress without restricting blood flow by clamping the hepatic pedicle, IR, rats underwent hepatic vascular occlusion intermittently for two complete cycles of 15 minutes of ischemia followed by 5 min of reperfusion, IR / HBO, rats underwent hepatic pedicle clamping and thereafter exposed to hyperbaric oxygen pressure of 2 absolute atmospheres for 60 minutes. We evaluated liver function through mitochondrial function, determined by the stages 3 and 4 of respiration, respiratory control ratio (RCR) and mitochondrial permeability transition (Swelling). Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were also quantified . We analyzed the results using the Mann-Whitney test and were considered significant all results with p <0.05. RESULTS: There were significant differences between the results of stage 3 in SHAM vs IR group ; of the stage 4 in the groups IR vs SHAM and SHAM vs IR /HBO; of the Respiratory Control Ratio (RCR) in the group IR vs IR / HBO ; of alanine aminotransferase in the groups IR vs SHAM , SHAM vs IR/HBO and IR vs IR / HBO; aspartate aminotransferase in the groups SHAM vs IR and SHAM vs IR / HBO. CONCLUSION: The whole analysis of the mitochondiral function indicators permits us to conclude that the hyperbaric oxygen therapy acted as a protective agent of the mitochondrial function, minimizing the ischemia-reperfusion injury of the hepatic parenchyma.

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[7] Mitochondrial respiratory control and the polarographic measurement of ADP : O ratios

Methods in Enzymology - Oxidation and Phosphorylation ◽

10.1016/0076-6879(67)10010-4 ◽

1967 ◽

pp. 41-47 ◽

Cited By ~ 1081

Author(s):

Ronald W. Estabrook

Keyword(s):

Respiratory Control ◽

Polarographic Measurement ◽

Mitochondrial Respiratory

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Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart

The International Journal of Biochemistry & Cell Biology ◽

10.1016/j.biocel.2011.08.008 ◽

2011 ◽

Vol 43 (12) ◽

pp. 1729-1738 ◽

Cited By ~ 106

Author(s):

Hélène Lemieux ◽

Severin Semsroth ◽

Herwig Antretter ◽

Daniel Höfer ◽

Erich Gnaiger

Keyword(s):

Oxidative Phosphorylation ◽

Human Heart ◽

Respiratory Control ◽

Mitochondrial Respiratory

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Characterization of mitochondria isolated from the freezing-tolerant larvae of the goldenrod gall fly (Eurosta solidaginis): substrate preferences, salt effects, and pH effects on warm- and cold-acclimated animals

Canadian Journal of Zoology ◽

10.1139/z85-057 ◽

1985 ◽

Vol 63 (2) ◽

pp. 373-379 ◽

Cited By ~ 9

Author(s):

James S. Ballantyne ◽

Kenneth B. Storey

Keyword(s):

Salt Concentration ◽

Respiratory Control ◽

Ph Effects ◽

Respiratory Control Ratio ◽

Eurosta Solidaginis ◽

Optimal Range ◽

Salt Effects ◽

Substrate Preferences ◽

Optimal Ph

The mitochondria of the freezing-tolerant larvae of the goldenrod gall fly (Eurosta solidaginis) have been isolated and characterized. Proline is the preferred substrate of mitochondria from both warm- and cold-acclimated animals based on state 3 rates. Lipid is used as a substrate by warm- and cold-acclimated mitochondria assayed at 20 °C, but not by the mitochondria from cold-acclimated animals assayed at 1 °C. Cold-acclimated mitochondria assayed at 1 °C have a higher and broader optimal range of salt concentration for the oxidation of proline based on the respiratory control ratio (RCR) than those from warm-acclimated animals oxidizing the same substrate at 20 °C. The optimal pH for warm-acclimated mitochondria oxidizing proline at 20 °C is low (6.2) based on the RCR, but rises to pH 7.0 in cold-acclimated animals at 1 °C. It is suggested that the broad optimal salt concentration in the cold-acclimated animals and the very low optimal pH in warm-acclimated animals are adaptations for survival in this freezing-tolerant larva.

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Mitochondrial respiratory control can compensate for intracellular O2 gradients in cardiomyocytes at low Po 2

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.00162.2002 ◽

2002 ◽

Vol 283 (3) ◽

pp. H871-H878 ◽

Cited By ~ 22

Author(s):

Eiji Takahashi ◽

Koji Asano

Keyword(s):

Oxygen Consumption ◽

Oxygen Diffusion ◽

Respiratory Control ◽

Oxygen Gradients ◽

Nadh Fluorescence ◽

Carbonyl Cyanide ◽

Intrinsic Regulation ◽

Electrical Pacing ◽

Mitochondrial Oxidative Metabolism ◽

Mitochondrial Respiratory

In isolated single cardiomyocytes with moderately elevated mitochondrial respiration, direct evidence for intracellular radial gradients of oxygen concentration was obtained by subcellular spectrophotometry of myoglobin (Mb). When oxygen consumption was increased by carbonyl cyanide m-chlorophenylhydrazone (CCCP) during superfusion of cells with 4% oxygen, Po 2 at the cell core dropped to 2.3 mmHg, whereas Mb near the plasma membrane was almost fully saturated with oxygen. Subcellular NADH fluorometry demonstrated corresponding intracellular heterogeneities of NADH, indicating suppression of mitochondrial oxidative metabolism due to relatively slow intracellular oxygen diffusion. When oxygen consumption was increased by electrical pacing in 2% oxygen, radial oxygen gradients of similar magnitude were demonstrated (cell core Po 2 = 2.6 mmHg). However, an increase in NADH fluorescence at the cell core was not detected. Because CCCP abolished mitochondrial respiratory control while it was intact in electrically paced cardiomyocytes, we conclude that mitochondria with intact respiratory control can sustain electron transfer with reduced oxygen supply. Thus mitochondrial intrinsic regulation can compensate for relatively slow oxygen diffusion within cardiomyocytes.

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