Inhibitory or scavenging action of ketoconazole and ciclopiroxolamine against reactive oxygen species released by primed inflammatory cells

AbstractObjective:To assess the effect of pomegranate extract on acute inflammation due to myringotomy.Design:Prospective, randomised study.Subjects:Thirty Sprague–Dawley rats were divided into three groups. Group one constituted controls. Group two underwent myringotomy. Group three underwent myringotomy and also received 100 µl/day pomegranate extract, via gavage, one day before and two days after surgery. Following sacrifice 48 hours after myringotomy, the animals' right ears were used to determine the concentration of reactive oxygen species, using the chemiluminescence method; left ears were used for histological study.Results:Reactive oxygen species levels were significantly decreased in group three compared with group two (p < 0.01). The density of inflammatory cells in group three was significantly less than that in group two (p < 0.01). Lamina propria thickness and vessel density were also significantly decreased in group three compared with group two (p < 0.01).Conclusion:Our results indicate that oral pomegranate extract decreases reactive oxygen species concentration and acute inflammation in the tympanic membrane after myringotomy.

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Involvement of reactive oxygen species and JNK in increased expression of MCP-1 and infiltration of inflammatory cells in pressure-overloaded rat hearts

Molecular Medicine Reports ◽

10.3892/mmr.2012.852 ◽

2012 ◽

Cited By ~ 2

Author(s):

Jiepin Wang

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen ◽

Inflammatory Cells ◽

Oxygen Species ◽

Rat Hearts

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Reactive Oxygen Species-Mediated Tumor Microenvironment Transformation: The Mechanism of Radioresistant Gastric Cancer

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/5801209 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 14

Author(s):

Huifeng Gu ◽

Tianhe Huang ◽

Yicheng Shen ◽

Yin Liu ◽

Fuling Zhou ◽

...

Keyword(s):

Gastric Cancer ◽

Reactive Oxygen Species ◽

Tumor Microenvironment ◽

Epithelial Mesenchymal Transition ◽

Reactive Oxygen ◽

Inflammatory Cells ◽

Inflammatory Factors ◽

Oxygen Species ◽

Mesenchymal Transition ◽

Development Of Gastric Cancer

Radioresistance is one of the primary causes responsible for therapeutic failure and recurrence of cancer. It is well documented that reactive oxygen species (ROS) contribute to the initiation and development of gastric cancer (GC), and the levels of ROS are significantly increased in patients with GC accompanied with abnormal expressions of multiple inflammatory factors. It is also well documented that ROS can activate cancer cells and inflammatory cells, stimulating the release of a variety of inflammatory cytokines, which subsequently mediates the tumor microenvironment (TME) and promotes cancer stem cell (CSC) maintenance as well as renewal and epithelial-mesenchymal transition (EMT), ultimately resulting in radioresistance and recurrence of GC.

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Development of an ultrafast fluorescent probe for specific recognition of hypochlorous acid and its application in live cells

RSC Advances ◽

10.1039/d1ra04082k ◽

2021 ◽

Vol 11 (40) ◽

pp. 24669-24672

Author(s):

Zhencai Xu ◽

Xiaofeng Wang ◽

Tingting Duan ◽

Rong He ◽

Fangwu Wang ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Fluorescent Probe ◽

Hypochlorous Acid ◽

Reactive Oxygen ◽

Inflammatory Cells ◽

Live Cells ◽

Oxygen Species ◽

Specific Recognition

Hypochlorous acid (HOCl), a highly potent oxidant of reactive oxygen species, plays critical roles in many physiological and pathological processes. In this work, we designed a simple coumarin-based fluorescent probe, Cou–HOCl, for detecting HOCl in inflammatory cells.

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The Injury and Therapy of Reactive Oxygen Species in Intracerebral Hemorrhage Looking at Mitochondria

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/2592935 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 35

Author(s):

Jie Qu ◽

Weixiang Chen ◽

Rong Hu ◽

Hua Feng

Keyword(s):

Reactive Oxygen Species ◽

Intracerebral Hemorrhage ◽

Structural Damage ◽

Mitochondrial Permeability Transition ◽

Reactive Oxygen ◽

Permeability Transition Pore ◽

Permeability Transition ◽

Inflammatory Cells ◽

Oxygen Species ◽

Major Health Problem

Intracerebral hemorrhage is an emerging major health problem often resulting in death or disability. Reactive oxygen species (ROS) have been identified as one of the major damaging factors in ischemic stroke. However, there is less discussion about ROS in hemorrhage stroke. Metabolic products of hemoglobin, excitatory amino acids, and inflammatory cells are all sources of ROS, and ROS harm the central nervous system through cell death and structural damage, especially disruption of the blood-brain barrier. We have considered the antioxidant system of the CNS itself and the drugs aiming to decrease ROS after ICH, and we find that mitochondria are key players in all of these aspects. Moreover, when the mitochondrial permeability transition pore opens, ROS-induced ROS release, which leads to extensive liberation of ROS and mitochondrial failure, occurs. Therefore, the mitochondrion may be a significant target for elucidating the problem of ROS in ICH; however, additional experimental support is required.

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