Identification of pork genome in commercial meat extracts for Halal authentication by SYBR green I real-time PCR

2011 ◽  
Vol 46 (5) ◽  
pp. 951-955 ◽  
Author(s):  
Reza Farrokhi ◽  
Raziallah Jafari Joozani
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sybr Green ◽  
Sybr Green I ◽  
Halal Authentication

Fast and sensitive quantitative detection of HIV DNA in whole blood leucocytes by SYBR green I real-time PCR assay

2007 ◽  
Vol 21 (5-6) ◽  
pp. 368-378 ◽  
Author(s):  
Anna Casabianca ◽  
Caterina Gori ◽  
Chiara Orlandi ◽  
Federica Forbici ◽  
Carlo Federico Perno ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Sybr Green ◽  
Sybr Green I ◽  
Quantitative Detection ◽  
Pcr Assay ◽  
Hiv Dna

scholarly journals Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs

2017 ◽  
Vol 61 (4) ◽  
pp. 427-432 ◽  
Author(s):  
Agnieszka Kędrak-Jabłońska ◽  
Sylwia Budniak ◽  
Marek Krupa ◽  
Anna Szczawińska ◽  
Monika Reksa ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Real Time ◽  
Real Time Pcr ◽  
Biological Samples ◽  
High Specificity ◽  
Sybr Green ◽  
Sybr Green I ◽  
Taqman Probe ◽  
Rdna Gene ◽  
Intercalating Dye

AbstractIntroduction:The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene ofListeriaspp. and thehlyA gene ofListeria monocytogenesin biological samples of the liver, brain, and blood.Material and Methods:Five strains ofL. monocytogenesand single strains of each speciesL. ivanovii,L. innocua,L. grayi,L. welshimeri,andL. seeligeriwere used for the experiments. Additionally, five strains of other species of bacteria were used for evaluation of the specificity of tests. In the first stage of the study SYBR Green I real-time PCRs, one allowing detection of the 23S rDNA gene and two based on the amplification thehlyA gene, were performed. In the next part, three TaqMan probe-based real-time PCRs allowing confirmation of belonging toListeriaspp. andL. monocytogeneswere conducted.Results:The observation of amplification curves in real-time PCRs enabled the detection of both genes. A high regression coefficient of 0.99 was found for all reactions. Specific amplification products were obtained for the 23S rDNA andhlyA genes which confirm their belonging toListeriaspp. andL. monocytogenes, respectively. Other microbial species did not reveal real-time PCR products.Conclusion:Both real-time PCR methods for the detection ofListeriaspp. andL. monocytogenesin biological samples demonstrated a significant sensitivity and high specificity.

A Real-Time PCR/SYBR Green I Method for the Rapid Quantification of Salmonella enterica in Poultry Meat

2013 ◽  
Vol 6 (4) ◽  
pp. 1004-1015 ◽  
Author(s):  
Caterina Agrimonti ◽  
Laura Bortolazzi ◽  
Elena Maestri ◽  
Anna Maria Sanangelantoni ◽  
Nelson Marmiroli
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Salmonella Enterica ◽  
Sybr Green ◽  
Sybr Green I ◽  
Poultry Meat ◽  
Rapid Quantification
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