Cloning and expression of the gene encoding an extracellular alkaline serine protease from Vibrio alginolyticus strain HY9901, the causative agent of vibriosis in Lutjanus erythopterus (Bloch)

2007 ◽  
Vol 30 (8) ◽  
pp. 493-500 ◽  
Author(s):  
S H Cai ◽  
Z H Wu ◽  
J C Jian ◽  
Y S Lu
Gene ◽  
1994 ◽  
Vol 145 (1) ◽  
pp. 149-150 ◽  
Author(s):  
Anna Milano ◽  
Pier Luigi Manachini ◽  
Carlo Parini ◽  
Giovanna Riccardi

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Chang Chang ◽  
Siyi Gong ◽  
Zhiping Liu ◽  
Qiaojuan Yan ◽  
Zhengqiang Jiang

Abstract Background Proteases are important for hydrolysis of proteins to generate peptides with many bioactivities. Thus, the development of novel proteases with high activities is meaningful to discover bioactive peptides. Because natural isolation from animal, plant and microbial sources is impractical to produce large quantities of proteases, gene cloning and expression of target protease are preferred. Results In this study, an alkaline serine protease gene (GsProS8) from Geobacillus stearothermophilus was successfully cloned and expressed in Bacillus subtilis. The recombinant GsProS8 was produced with high protease activity of 3807 U/mL after high cell density fermentation. GsProS8 was then purified through ammonium sulfate precipitation and a two-step chromatographic method to obtain the homogeneous protease. The molecular mass of GsProS8 was estimated to be 27.2 kDa by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and 28.3 kDa by gel filtration. The optimal activity of GsProS8 was found to be pH 8.5 and 50 °C, respectively. The protease exhibited a broad substrate specificity and different kinetic parameters to casein and whey protein. Furthermore, the hydrolysis of whey protein using GsProS8 resulted in a large amount of peptides with high angiotensin-I-converting enzyme (ACE) inhibitory activity (IC50 of 0.129 mg/mL). Conclusions GsProS8 could be a potential candidate for industrial applications, especially the preparation of antihypertensive peptides.


Gene ◽  
1995 ◽  
Vol 152 (1) ◽  
pp. 59-63 ◽  
Author(s):  
Yong Tae Kwon ◽  
Jin Oh Kim ◽  
Sun Young Moon ◽  
Young Dong Yoo ◽  
Hyune Mo Rho

2018 ◽  
Vol 99 (4) ◽  
pp. 365-373
Author(s):  
Nguyen Thi Anh Thu ◽  
Ngo Thi Tuong Chau ◽  
Le Van Thien ◽  
Nguyen Duc Huy ◽  
Nguyen Tran Me Khue ◽  
...  

10.1038/76088 ◽  
2000 ◽  
Vol 25 (2) ◽  
pp. 213-216 ◽  
Author(s):  
Heiko Witt ◽  
Werner Luck ◽  
Hans Christian Hennies ◽  
Martin Claßen ◽  
Andreas Kage ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document