Estimation of surface labelling on whole, critical point dried, cells by microprobe analysis in the scanning electron microscope

1981 ◽  
Vol 121 (3) ◽  
pp. 329-336 ◽  
Author(s):  
I. Gwynn
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Critical Point ◽  
Microprobe Analysis ◽  
Surface Labelling ◽  
Scanning Electron

Pulmonary microcirculation: tubules rather than sheet and post

1982 ◽  
Vol 53 (2) ◽  
pp. 510-515 ◽  
Author(s):  
W. G. Guntheroth ◽  
D. L. Luchtel ◽  
I. Kawabori
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Critical Point ◽  
Ringer Solution ◽  
Basic Component ◽  
Pulmonary Vasculature ◽  
Critical Point Drying ◽  
Pulmonary Microcirculation ◽  
Scanning Electron

We examined latex casts of the pulmonary microcirculation with the scanning electron microscope (SEM). Mature rats were anesthetized and ventilated; the pulmonary vasculature was washed out with lactated Ringer solution and then filled with a mixture of Geon latexes. The airways were filled with glutaraldehyde with resulting transmural vascular pressures of 10 cmH2O. After critical-point drying and corrosive removal of the lung tissue, SEM studies of the vascular replicas revealed two distinct patterns of pulmonary microcirculation: 1) sparse, long, tubular capillaries that comprise the thin subpleural layer and appear as “filler” in the peribronchial spaces; and 2) alveolar microcirculation that is composed of tightly matted, intersecting tubules, shorter but of the same diameter as type 1, in spherical array in two layers. The alveolar capillaries at low magnification appear superficially as sheets; however, the detailed morphology is not consistent with the sheet-and-post model. We conclude that the basic component of the pulmonary microcirculation is tubular and not different from other capillary beds except in density.

A comparison of microstructures of Cheddar cheese curd manufactured with calf rennet, bovine pepsin, and porcine pepsin

1976 ◽  
Vol 43 (1) ◽  
pp. 113-115 ◽  
Author(s):  
M. F. Eino ◽  
D. A. Biggs ◽  
D. M. Irvine ◽  
D. W. Stanley
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Critical Point ◽  
Cheddar Cheese ◽  
Microscope Examination ◽  
Porcine Pepsin ◽  
Electron Microscope Examination ◽  
Critical Point Drying ◽  
Cheese Curd ◽  
Scanning Electron

SummaryCalf rennet, bovine pepsin, and porcine pepsin were used to produce cheese curd, using the same milk and lactic culture for each. Specimens were prepared for scanning electron microscope examination by a modified critical-point drying technique.From examination of the micrographs, the curd made with bovine and porcine pepsin were similar in structure and in orientation of the coagulated protein, whereas the curd produced with rennet was different, having a more compact and organized structure.

Excipuloid stroma ontogeny in Peziza quelepidotia

1976 ◽  
Vol 54 (17) ◽  
pp. 2049-2054
Author(s):  
K. L. O'donnell ◽  
G. R. Hooper
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Critical Point ◽  
Light Microscope ◽  
Scanning Electron

Excipuloid stroma ontogeny in Peziza quelepidotia Korf & O'Donnell was investigated. The scanning electron microscope was used to examine whole specimens from the initials up to maturity. In addition, the internal features of cryofractured, critical-point-dried stroma of various stages were investigated and compared with thick-sectioned, plastic-embedded material viewed in the light microscope. A comparison of these indeterminate vegetative structures with stroma and sclerotia was made.

Microscopic study of the plerocercoid larva of Otobothrium (Trypanorhyncha)

1995 ◽  
Vol 53 ◽  
pp. 1026-1027
Author(s):  
N. Abdou ◽  
R. A. Heckmann ◽  
J. S. Gardner ◽  
A. A. Ashour
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Critical Point ◽  
Microscopic Study ◽  
Red Sea ◽  
Tannic Acid ◽  
Posterior Margin ◽  
Different Types ◽  
Sea Fish ◽  
Scanning Electron

This study represents the first scanning electron microscope (SEM) evaluation of cestode larvae infecting Red Sea fish. General structures of the larvae and different types of microtriches on the integument are described.Plerocercoids of Trypanorhyncha were collected from the mesenteries and liver of Cephalopholis oligostricta. The capsule of each larva was opened, the plerocercoid was removed and fixed in 10% formalin. Following buffer (pH. 7.3) wash, they were fixed in OSO4 12 hr, washed in buffer, immersed in 2% tannic acid 8 hr, washed in buffer, fixed again in OSO4 2 hr, buffer washed, dehydrated, critical point dried, and sputter coated with gold.The plerocercoids were identified as belonging to the genus Otobothrium. Each organism was divided into a scolex and body. The scolex consisted of two large bothria, each bothrium was nearly round in outline and possessed two circular invaginations on its posterior margin. On the apex of the scolex, four extended tentacles covered with hooks were observed (Fig 1).

The Fine Surface Structure of the Male Genital Bulb of the Dwarf Spider Catabrithorax Plumosus

1988 ◽  
Vol 46 ◽  
pp. 282-283
Author(s):  
C Cady ◽  
R. V. Cyrus ◽  
J. L. Kaspar
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Critical Point ◽  
Amyl Acetate ◽  
Critical Point Drying ◽  
Fine Surface ◽  
Genital Structure ◽  
Ethanol Series ◽  
Scanning Electron ◽  
Male Genital

The spider Catabrithorax plumosus (Emerton) family Linyphiidae, subfamily Erigoninae is a member of an abundant yet reclusive group known as the dwarf spiders. Collectively, these are the smallest spiders in North America. Most measure only 1-3 mm in length, creating difficulties in indentification. The morphology of the male genital bulb, located on the distal pedipalp, is of great importance to the taxonomist in identifying spiders to the species level. This study examines the complex male genital structure in both the expanded and unexpended condition by means of the Scanning Electron Microscope (SEM).Pedipalps were removed at the trochanter-femur articulation, dehydrated in 70% ethanol and expanded by Immersion in lactic acid for 6-8 h. After complete expansion a graded amyl acetate series preceeded critical point drying. Unexpanded palps were dehydrated in an ethanol series followed by a graded amyl acetate series in ethanol. Specimens were critical point dried from CO2 and given an evaporated coating of carbon-gold. Specimens were examined in a Hitachi HHS-2R Scanning Electron Microscope operating at an accelerating voltage of 10 kV.

Electron Microscopic studies on the antennal sensilla and the pheromone-producing glands of two important oil seed pests

1990 ◽  
Vol 48 (3) ◽  
pp. 570-571
Author(s):  
Kaiser Jamil ◽  
K.N. Jyothi ◽  
A.L. Prasuna
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Critical Point ◽  
Phosphate Buffer ◽  
Antennal Sensilla ◽  
Olfactory Perception ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Sensory Mechanisms ◽  
Scanning Electron

Sensory mechanisms underlying insect chemical communication have been given considerable attention in recent years. A study on the ultrastructural details of the antennal appendages and the pheromone producing structures of Achoea janata L. and Ophiusa algira were studied with scanning electron microscope in order to understand the mechanisms of olfactory perception and pheromone release.For fixation of pheromone producing glands of A.Janata and O.Algira the posterior region of the abdomen was pressed sufficiently and flooded with carnoys fluid for complete excedation of the 9th abdominal along with the ovipositor. The above specimens as well as the antennal appendages were then fixed in 4% glutaraldehyde in phosphate buffer of 7.2 pH dehydrated in increasing grades of ethylalcohol and critical point dried. The specimens were then coated with gold in vacuum evaporator and observed in a Hitachi S-520 evaporator and observed in a Hitachi S-520 SEM operated at 20KV.

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