Microscopic study of the plerocercoid larva of Otobothrium (Trypanorhyncha)

Author(s):  
N. Abdou ◽  
R. A. Heckmann ◽  
J. S. Gardner ◽  
A. A. Ashour

This study represents the first scanning electron microscope (SEM) evaluation of cestode larvae infecting Red Sea fish. General structures of the larvae and different types of microtriches on the integument are described.Plerocercoids of Trypanorhyncha were collected from the mesenteries and liver of Cephalopholis oligostricta. The capsule of each larva was opened, the plerocercoid was removed and fixed in 10% formalin. Following buffer (pH. 7.3) wash, they were fixed in OSO4 12 hr, washed in buffer, immersed in 2% tannic acid 8 hr, washed in buffer, fixed again in OSO4 2 hr, buffer washed, dehydrated, critical point dried, and sputter coated with gold.The plerocercoids were identified as belonging to the genus Otobothrium. Each organism was divided into a scolex and body. The scolex consisted of two large bothria, each bothrium was nearly round in outline and possessed two circular invaginations on its posterior margin. On the apex of the scolex, four extended tentacles covered with hooks were observed (Fig 1).

1980 ◽  
Vol 59 (2) ◽  
pp. 124-128 ◽  
Author(s):  
Y. Galindo ◽  
K. McLachlan ◽  
Z. Kasloff

A silver-plating technique was developed in an effort to produce good mechanical bonding characteristics between stainless steelpins and amalgam. Metallographic microscope and scanning electron microscope (SEM) studies were made to assess the presence, or otherwise, of such a bond between (a) the silver layer plating and the surface of the stainless steel pins, and (b) and silver plating and the amalgam. Unplated stainless steel and sterling silver pins were used as a control and as a comparison, respectively. A "rubbing" technique of condensation was devised to closely adapt amalgam to the pins. It is concluded that there is strong evidence for the existence of a good bond between the plated pins and amalgam. The mechanical performance of the bond is discussed elsewhere. 1.


1982 ◽  
Vol 53 (2) ◽  
pp. 510-515 ◽  
Author(s):  
W. G. Guntheroth ◽  
D. L. Luchtel ◽  
I. Kawabori

We examined latex casts of the pulmonary microcirculation with the scanning electron microscope (SEM). Mature rats were anesthetized and ventilated; the pulmonary vasculature was washed out with lactated Ringer solution and then filled with a mixture of Geon latexes. The airways were filled with glutaraldehyde with resulting transmural vascular pressures of 10 cmH2O. After critical-point drying and corrosive removal of the lung tissue, SEM studies of the vascular replicas revealed two distinct patterns of pulmonary microcirculation: 1) sparse, long, tubular capillaries that comprise the thin subpleural layer and appear as “filler” in the peribronchial spaces; and 2) alveolar microcirculation that is composed of tightly matted, intersecting tubules, shorter but of the same diameter as type 1, in spherical array in two layers. The alveolar capillaries at low magnification appear superficially as sheets; however, the detailed morphology is not consistent with the sheet-and-post model. We conclude that the basic component of the pulmonary microcirculation is tubular and not different from other capillary beds except in density.


2011 ◽  
Vol 361-363 ◽  
pp. 288-295
Author(s):  
De Wei Chen ◽  
Xiao Ling Ge ◽  
Quen Tin Shi ◽  
Jiu Wang Tian

Using scanning electron microscope, the microscopic pictures of the surface on sediment have been obtained from the sub-micron titanium dioxide suspensions without dispersant or adding different types of dispersants. Deposits of titanium dioxide suspension have been observed and the mechanism of the dispersant has been analyzed. Meanwhile, deposits of titanium dioxide gray distribution figure have been gained by self-compiled Matlab program, which proved that the surface sediments is fractal, and the fractal dimension has been calculated under different conditions. The rules between suspension stability and fractal dimension have been achieved.


1976 ◽  
Vol 43 (1) ◽  
pp. 113-115 ◽  
Author(s):  
M. F. Eino ◽  
D. A. Biggs ◽  
D. M. Irvine ◽  
D. W. Stanley

SummaryCalf rennet, bovine pepsin, and porcine pepsin were used to produce cheese curd, using the same milk and lactic culture for each. Specimens were prepared for scanning electron microscope examination by a modified critical-point drying technique.From examination of the micrographs, the curd made with bovine and porcine pepsin were similar in structure and in orientation of the coagulated protein, whereas the curd produced with rennet was different, having a more compact and organized structure.


1981 ◽  
Vol 59 (5) ◽  
pp. 683-688 ◽  
Author(s):  
R. W. Rose Jr. ◽  
C. Gerald Van Dyke ◽  
C. B. Davey

Three different types of ectomycorrhizae found in the Southeastern United States on Eucalyptus nova-anglica and identified as being formed by Cenococcum geophilum, Pisolithus tinctorius, and Scleroderma geaster were examined with a scanning electron microscope (SEM). In overall appearance the three types of mycorrhizae could be distinguished easily from each other, particularly C. geophilum, which had a relatively smooth, undulate surface in comparison with the other two types. Detailed descriptions and comparisons of these ectomycorrhizae are given and the value of SEM in characterizing ectomycorrhizae is discussed


2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
N. Lakshmi Kruthika ◽  
G. Bhaskar Raju ◽  
S. Prabhakar

Structured TiO2 nanotubes were grown on 2 mm thick titanium sheet by anodization of titanium in ethylene glycol medium containing 0.025 M NaF. The morphology of TiO2 nanotubes (TNT) was characterized using field emission scanning electron microscope. The potential of TNT as anode and also as photocatalyst for the degradation of tannic acid was studied. The mineralization of tannic acid was measured in terms Total Organic Carbon (TOC). Only 50% of TOC could be removed by exposing the tannic acid solution to UV-radiation (photolysis), whereas it was improved to 70% by electrooxidation (EO) using TNT as anode. Maximum degradation of 83% was achieved when electrooxidation was conducted under the influence of UV-radiation (photoelectrocatalytic process (PEC)). Among the electrolytes tried, Na2SO4 was observed to be very effective for the degradation of tannic acid. The kinetics of tannic acid degradation by photoelectrocatalytic process was found to follow zero-order rate expression.


2013 ◽  
Vol 73 (3) ◽  
pp. 663-668 ◽  
Author(s):  
DE. Euzébio ◽  
GF. Martins ◽  
TM. Fernandes-Salomão

The ant Atta robusta is endemic to the “restinga” ecosystems where it has an important role in the dynamics of seed dispersal. Despite its importance, A. robusta is considered a threatened species. In this study we analyzed the antennal sensory organs of two different populations of A. robusta (from the cities of São Mateus and Maricá in in Espírito Santo and Rio de Janeiro States, respectively) using a scanning electron microscope (SEM). SEM revealed different types of sensilla in the A. robusta antennae, i.e., curved and straight trichoid, basiconic, ampullacea and coeloconic, which were highly abundant found in the distal flagellomeres (F) compared with other antenna regions. There were differences in samples collected from two locations in terms of the sensilla number and length. The average numbers of straight and curved trichoid sensillae numbers were different in F9 and F8, respectively, while the average length of the curved trichoid sensilla was only different in F9. These variations in sensory organs between two populations of A. robusta may indicate an adaptation of this species to different environmental conditions. The number of straight trichoid sensilla was only significantly different in F9.


Minerals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1435
Author(s):  
Olev Vinn

Aragonite plays an important role in the biomineralization of serpulid polychaetes. Aragonitic structures are present in a wide range of serpulid species, but they mostly belong to one clade. Aragonitic structures are present in a wide range of marine environments, including the deep ocean. Aragonitic tube microstructures were studied using a scanning electron microscope. X-ray powder diffraction was used to identify the aragonite. Aragonite is used to build five different types of microstructures in serpulid tubes. The most common aragonitic irregularly oriented prismatic structure (AIOP) is also, evolutionarily, the most primitive. Some aragonitic microstructures, such as the spherulitic prismatic (SPHP) structure, have likely evolved from the AIOP structure. Aragonitic microstructures in serpulids are far less numerous than calcitic microstructures, and they lack the complexity of advanced calcitic microstructures. The reason why aragonitic microstructures have remained less evolvable than calcitic microstructures is currently unknown, considering their fit with the current aragonite sea conditions (Paleogene–recent).


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