A simplified ‘sandwich’ technique for in situ embedding and perpendicular sectioning of monolayer cultures of human skin fibroblasts

1987 ◽  
Vol 147 (2) ◽  
pp. 209-212 ◽  
Author(s):  
P. J. Govaerts ◽  
I. I. Bernaer ◽  
M. V. L. Caju ◽  
W. A. Jacob
Keyword(s):  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Sandwich Technique ◽  
Monolayer Cultures ◽  
In Situ Embedding

1,25-DIHYDROXYCHOLECALCIFEROL STIMULATES CONVERSION OF ANDROSTENEDIONE INTO OESTRONE BY HUMAN SKIN FIBROBLASTS IN CULTURE

1986 ◽  
Vol 110 (1) ◽  
pp. R1-R4 ◽  
Author(s):  
M.B. Hodgins ◽  
S. Murad
Keyword(s):  
Bone Resorption ◽  
Bone Loss ◽  
Human Skin ◽  
Reductase Inhibitor ◽  
Skin Fibroblasts ◽  
Aromatase Activity ◽  
Human Skin Fibroblasts ◽  
Maximal Stimulation ◽  
Monolayer Cultures ◽  
Physiological Regulator

ABSTRACT Pre-incubation of monolayer cultures of human skin fibroblasts with 1,25-dihydroxycholecalciferol (1,25-D3; 0.1-10 nmol/l) increased the rate of conversion of androstenedione into oestrone (aromatase activity) when measured subsequently in the presence of a 5α-reductase inhibitor (10 umol/l). Maximal stimulation (14- to 89-fold with 10 nmol 1,25-D3/l) occurred 12 h after addition of the hormone and was maintained for up to 48 h. Stimulation was prevented by cycloheximide. In one cell line high 1,25-D3 concentrations (>30 nmol/l)prevented the increase in aromatase activity; this did not appear to result from direct enzyme inhibition by 1,25-D3. The possibility is considered that 1,25-D3 could act as a physiological regulator of peripheral aromatase. As oestrogens can prevent postmenopausal bone loss, it is speculated that 1,25-D3 might protect against bone resorption by maintaining peripheral oestrogen biosynthesis.

Electron microscopy of human skin fibroblasts in situ during growth in culture

1975 ◽  
Vol 92 (2) ◽  
pp. 383-393 ◽  
Author(s):  
A LUCKY ◽  
M MAHONEY ◽  
R BARRNETT ◽  
L ROSENBERG
Keyword(s):  
Electron Microscopy ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

scholarly journals Modifications of proteoglycans extracted from monolayer cultures of young and senescent human skin fibroblasts

FEBS Letters ◽  
1997 ◽  
Vol 420 (2-3) ◽  
pp. 175-178 ◽  
Author(s):  
Alberto Passi ◽  
Riccardo Albertini ◽  
Francesco Campagnari ◽  
Giancarlo De Luca
Keyword(s):  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Monolayer Cultures

117-LB: Nonviral Direct Reprogramming of Human Skin Fibroblasts into Hormone-Expressing ß-Like Cell Clusters

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 117-LB
Author(s):  
LUKE R. LEMMERMAN ◽  
MARIA ANGELICA RINCON-BENAVIDES ◽  
SARAH A. TERSEY ◽  
BRITANI N. BLACKSTONE ◽  
HEATHER M. POWELL ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Fibroblasts ◽  
Direct Reprogramming ◽  
Human Skin Fibroblasts ◽  
Cell Clusters

Protective Effects of Green Tea Seed Extract against UVB-irradiated Human Skin Fibroblasts

2014 ◽  
Vol 43 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Ok Kyung Kim ◽  
Da-Eun Nam ◽  
Min-Jae Lee ◽  
Namgil Kang ◽  
Jae-Youn Lim ◽  
...  
Keyword(s):  
Human Skin ◽  
Green Tea ◽  
Seed Extract ◽  
Skin Fibroblasts ◽  
Protective Effects ◽  
Human Skin Fibroblasts

The intralysosomal pH in cultured human skin fibroblasts in relation to cystine accumulation in patients with cystinosis

1983 ◽  
Vol 116 (1) ◽  
pp. 154-161 ◽  
Author(s):  
Ronald P.J. Oude Elferink ◽  
Erik Harms ◽  
Anneke Strijland ◽  
Joseph M. Tager
Keyword(s):  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

The Effect of Practolol, In Vitro Generated Metabolites of Practolol and Chemical Analogues on the Rate of Growth of Human Skin Fibroblasts

1984 ◽  
Vol 12 (2) ◽  
pp. 89-97
Author(s):  
Graham R. Elliott ◽  
H.E. Amos ◽  
James W. Bridges
Keyword(s):  
Human Skin ◽  
Psoriasis Patient ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Cell Type ◽  
Normal Human Skin ◽  
Rate Of Growth ◽  
Structural Analogues ◽  
Normal Human

The rate of growth of normal human skin fibroblasts was inhibited in a dose related, reversible, fashion by practolol (N-4-(2-hydroxy)-3 (1-methyl)-aminopropoxyphenylacetamine) (ID50 1.35 ± 0.14 x 10-3M), propranolol (1-(isopropylamino)-3(1-naphthyl-oxy)-2-propranolol) (ID50 0.145 ± 0.02 x 10-3M) and paracetamol (N-(4-hydroxyphenyl) acetamide) (ID50 0.85 ± 0.2 x 10-3M). Skin fibroblasts isolated from a psoriasis patient were more sensitive towards practolol (ID50 0.48 ± 0.14 x 10-3M) and propranolol (ID50 0.032 ± 0.002 x 10-3M), but less sensitive towards paracetamol (ID50 1.3 ± 0.07 x 10-3M). In vitro generated metabolites of practolol, using normal or Arochlor 1254-pretreated hamster liver preparations, and structural analogues of practolol had no effect upon the growth of either cell type.

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