Isolation and characterization of the major vegetative RNA polymerase of Streptomyces coelicolor A3(2); renaturation of a sigma subunit using GroEL

1992 ◽  
Vol 6 (9) ◽  
pp. 1133-1139 ◽  
Author(s):  
Kelly L. Brown ◽  
Steven Wood ◽  
Mark J. Buttner
Keyword(s):  
Rna Polymerase ◽  
Streptomyces Coelicolor ◽  
Isolation And Characterization ◽  
Sigma Subunit

scholarly journals Isolation and characterization of transducing phage coding for sigma subunit of Escherichia coli RNA polymerase.

1979 ◽  
Vol 76 (11) ◽  
pp. 5789-5793 ◽  
Author(s):  
C. A. Gross ◽  
F. R. Blattner ◽  
W. E. Taylor ◽  
P. A. Lowe ◽  
R. R. Burgess
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Isolation And Characterization ◽  
Sigma Subunit

Isolation and characterization of a mitochondrial RNA polymerase from Drosophila melanogaster

1987 ◽  
Vol 65 (2) ◽  
pp. 173-182 ◽  
Author(s):  
Michael Goldenthal ◽  
James T. Nishiura
Keyword(s):  
Drosophila Melanogaster ◽  
Rna Polymerase ◽  
Nuclear Dna ◽  
Gradient Centrifugation ◽  
Mitochondrial Rna ◽  
Mitochondrial Rna Polymerase ◽  
Isolation And Characterization ◽  
Cscl Gradient ◽  
Nuclear Rna

A DNA-dependent RNA polymerase was solubilized from sucrose gradient isolated, DNase-treated mitochrondria of Drosophila melanogaster. The isolated mitochondria were not detectably contaminated with nuclear DNA as shown by CsCl gradient centrifugation and polylysine Kieselguhr chromatography. The detergent-solubilized RNA polymerase was sensitive to rifampicin, resistant to α-amanitin, had an apparent molecular mass of about 60 kilodaltons, and displayed a tendency to aggregate, both in crude extracts or when purified. The mitochondrial RNA polymerase could be distinguished from nuclear RNA polymerases on the basis of size, salt optima, rifampicin sensitivity, and α-amanitin resistance.

scholarly journals Genome sequence and characterization of five bacteriophages infecting Streptomyces coelicolor and Streptomyces venezuelae: Alderaan, Coruscant, Dagobah, Endor1 and Endor2

2020 ◽  
Author(s):  
Aël Hardy ◽  
Vikas Sharma ◽  
Larissa Kever ◽  
Julia Frunzke
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Streptomyces Coelicolor ◽  
Morphological Differentiation ◽  
Viral Diversity ◽  
Isolation And Characterization ◽  
Phage Evolution ◽  
Transmission Electron ◽  
Family Based

AbstractStreptomyces are well-known antibiotic producers, and are also characterized by a complex morphological differentiation. Streptomyces, like all bacteria, are confronted with the constant threat of phage predation, which in turn shapes bacterial evolution. However, despite significant sequencing efforts recently, relatively few phages infecting Streptomyces have been characterized compared to other genera. Here, we present the isolation and characterization of five novel Streptomyces phages. All five phages belong to the Siphoviridae family, based on their morphology as determined by transmission electron microscopy. Genome sequencing revealed that four of them were temperate phages, while one had a lytic lifestyle. Moreover, one of the newly sequenced phages shows very little homology to already described phages, highlighting the still largely untapped viral diversity. Altogether, this study expands the number of characterized phages of Streptomyces and sheds light on phage evolution and phage-host dynamics in Streptomyces.

scholarly journals Isolation and Characterization of the DNA-dependent RNA Polymerase of Rhizobium leguminosarum 300

Microbiology ◽  
1981 ◽  
Vol 125 (2) ◽  
pp. 301-309
Author(s):  
W. LOTZ ◽  
H. FEES ◽  
W. WOHLLEBEN ◽  
H. J. BURKARDT
Keyword(s):  
Rna Polymerase ◽  
Rhizobium Leguminosarum ◽  
Isolation And Characterization

Isolation and characterization of macronuclei of Paramecium caudatum infected with the macronucleus-specific bacterium Holospora obtusa

1985 ◽  
Vol 73 (1) ◽  
pp. 389-398
Author(s):  
M. Freiburg
Keyword(s):  
Rna Polymerase ◽  
Polymerase Activity ◽  
Proteinase Activity ◽  
Paramecium Caudatum ◽  
Isolation And Characterization ◽  
Rna Polymerase Activity

Macronuclei from Paramecium caudatum infected with Holospora obtusa may be isolated on sucrose step gradients. Macronuclei containing primarily infectious forms can be separated from those bearing predominantly reproductive forms. RNA polymerase activity in infected macronuclei is greater by a factor of 5 than that in uninfected macronuclei. Proteinase activity is also significantly higher.

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