Characterization of RNA polymerse and two sigma-factor genes from Mycobacterium smegmatis

1995 ◽  
Vol 15 (2) ◽  
pp. 355-366 ◽  
Author(s):  
Mima Predich ◽  
Laurence Doukhan ◽  
Gopalan Nair ◽  
Issar Smith
Keyword(s):  
Sigma Factor ◽  
Mycobacterium Smegmatis

Purification and characterization of C28–55 fatty acids from Mycobacterium smegmatis

1985 ◽  
Vol 31 (3) ◽  
pp. 214-219 ◽  
Author(s):  
Neeraja Sathyamoorthy ◽  
Nilofer Qureshi ◽  
Kuni Takayama
Keyword(s):  
Fatty Acids ◽  
Mycobacterium Smegmatis ◽  
High Performance ◽  
Mycolic Acid ◽  
Argentation Thin Layer Chromatography ◽  
Mycolic Acids ◽  
Electron Impact Mass ◽  
Layer Chromatography ◽  
Impact Mass

The nonmycolic C16 to C55 fatty acids obtained from Mycobacterium smegmatis ATCC 356 by saponification were enriched with respect to the C28 to C55 acids by successive chromatography on silicic acid and Sephadex LH-20 columns. These partially purified fatty acids were then derivatized to the p-bromophenacyl ester and further fractionated by argentation thin-layer chromatography and reverse-phase high-performance liquid chromatography into their individual components.The esters were characterized by electron impact mass spectrometry. Two structural series of C28:1 to C42:1 and C45:2 to C55:2 fatty acids were identified as possible precursors of the monoenyl and dienyl mycolic acids, respectively. These acids were structurally related to the α-alkylhydroxyl group of the corresponding mycolic acid. The results suggest that these C28 to C55 fatty acids (meromycolic acids) of M. smegmatis might be precursors of mycolic acids.

scholarly journals Isolation and characterization of Escherichia coli mutants that lack the heat shock sigma factor sigma 32.

1988 ◽  
Vol 170 (8) ◽  
pp. 3640-3649 ◽  
Author(s):  
Y N Zhou ◽  
N Kusukawa ◽  
J W Erickson ◽  
C A Gross ◽  
T Yura
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Sigma Factor ◽  
Isolation And Characterization ◽  
Sigma 32

scholarly journals Biochemical characterization of a glycosyltransferase Gtf3 from Mycobacterium smegmatis: a case study of improved protein solubilization

3 Biotech ◽  
2020 ◽  
Vol 10 (10) ◽  
Author(s):  
Mahfoud Bakli ◽  
Loukmane Karim ◽  
Nassima Mokhtari-Soulimane ◽  
Hafida Merzouk ◽  
Florence Vincent
Keyword(s):  
Mycobacterium Smegmatis ◽  
Biochemical Characterization ◽  
Protein Solubilization

scholarly journals Characterization of Sigma Factor Genes in Streptomyces lividans TK24 Using a Genomic Library-Based Approach for Multiple Gene Deletions

2018 ◽  
Vol 9 ◽  
Author(s):  
Yuriy Rebets ◽  
Konstantinos C. Tsolis ◽  
Elísabet Eik Guðmundsdóttir ◽  
Joachim Koepff ◽  
Beata Wawiernia ◽  
...  
Keyword(s):  
Sigma Factor ◽  
Genomic Library ◽  
Streptomyces Lividans ◽  
Gene Deletions ◽  
Multiple Gene
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