Further evidence by gene dosage for the regional assignment of erythrocyte acid phosphatase (ACPI) and malate dehydrogenase (MDHI) loci on chromosome 2p

Thirteen loci from 11 enzyme systems were identified among full-sib and half-sib progeny of blue and Engelmann spruce. Eleven of the loci were expressed in bud, embryo, and megagametophyte tissue; the remaining two loci were expressed only in embryo and megagametophyte tissue. There were no mobility differences observed between loci expressed in seed and bud tissues. The mode of inheritance for 10 of the loci was confirmed based on progeny genotypic distributions. For the two loci not expressed in bud tissue, acid phosphatase (Acp-2) and diaphorase (Dia-2), inheritance was inferred from pooled segregation ratios of megagametophytes from open-pollinated seed from heterozygous females. The inheritance of glutamate oxaloacetate transaminase (Got-3) was also inferred from segregation ratios and diploid embryo phenotypes of open-pollinated progeny owing to a lack of variability at this locus among the 40 parents in the mating design. Two loci, aldolase (Ald) and malate dehydrogenase (Mdh-2), were monomorphic among the 20 parents of both species. Key words: isozymes, Engelmann spruce, blue spruce, Picea.

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GENETICS OF ISOZYMES AND ANALYSIS OF ISOZYMES LINKAGE AND MORPHOLOGICAL LOCI IN SUNFLOWER (Helianthus annuus L.) / GENETICA DE ISOFERMENTOS Y EL ACOMPLAMIENTO DE LÓCUSES MORFOLOGICOS E ISOFERMENTICOS EN GIRASOL / GENETIQUE DES ISOFERMENTS ET LIAISON DES LOCUS MORPHOLOGIQUES ET CEUX-CI D’ISOFERMENTS AU TOURNESOL

Helia ◽

10.1515/helia.2000.23.33.65 ◽

2000 ◽

Vol 23 (33) ◽

pp. 65-76

Author(s):

V.V. Kirichenko ◽

V.N. Popov

Keyword(s):

Acid Phosphatase ◽

Linkage Group ◽

Helianthus Annuus ◽

Malate Dehydrogenase ◽

Morphological Traits ◽

Fertility Restoration ◽

Linkage Groups ◽

Esterase Loci ◽

Mature Seeds ◽

Male Fertility Restoration

SUMMARY The genetics of anodal esterase (Est), cathodal esterase (cEst), cathodal acid phosphatase (cAcp) and malate dehydrogenase (Mdh) has been studied in mature seeds and leaves (genetics of cAcp and Mdh has not been studied in leaves) of sunflower (Helianthus annuus L.). A total of ten loci (four loci of anodal esterase, two loci of cathodal esterase, three loci of malate dehydrogenase and one locus of cathodal acid phosphatase) have been identified and described. Five esterase loci (Est1, Est2, Est3, Est4, cEst5), three malate dehydrogenase loci and one locus of cathodal acid phosphatase are expressed in seeds. Three esterase loci (Est2, cEst5 and cEst6) are expressed in leaves. The analysis of linkage between these loci has been made. Two linkage groups have been found. The sequence of the loci in the first linkage group was Mdh2-Est1- Est2-Est3-cEst5. In the second linkage group it was Est4-cAcp1. Linkages have been analyzed between three isoenzymatic loci expressed in leaves and between two loci controlling morphological traits (branched stem and male fertility restoration). The linkage between morphological traits and isoenzymatic loci has not been revealed. It has been revealed in Br-Rf pair.

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Further evidence for the assignment of the red cell acid phosphatase gene (ACP1) to the short arm of chromosome 2 from gene dosage effect

Cytogenetic and Genome Research ◽

10.1159/000130622 ◽

1976 ◽

Vol 16 (1-5) ◽

pp. 326-327

Author(s):

R.E. Magenis ◽

R.D. Koler ◽

E. Lovrien ◽

R.H. Bigley ◽

M.C. DuVal ◽

...

Keyword(s):

Acid Phosphatase ◽

Gene Dosage ◽

Dosage Effect ◽

Red Cell ◽

Chromosome 2 ◽

Gene Dosage Effect ◽

I Gene

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Regional assignment of the ADA locus on 20q13.2→qter by gene dosage studies

Cytogenetic and Genome Research ◽

10.1159/000131481 ◽

1980 ◽

Vol 27 (2-3) ◽

pp. 187-189 ◽

Cited By ~ 21

Author(s):

T. Philip ◽

G. Lenoir ◽

M.O. Rolland ◽

I. Philip ◽

M. Hamet ◽

...

Keyword(s):

Gene Dosage ◽

Regional Assignment

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Confirmation of regional assignment of nucleoside phosphorylase to band 14q13 by gene-dosage studies

Human Genetics ◽

10.1007/bf00399402 ◽

1979 ◽

Vol 50 (3) ◽

pp. 341-343 ◽

Cited By ~ 3

Author(s):

B. Dallapiccola ◽

M. Magnani ◽

M. Dach� ◽

P. L. Giorgi

Keyword(s):

Gene Dosage ◽

Nucleoside Phosphorylase ◽

Regional Assignment

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Gene dosage evidence for the regional assignment of the GOTs structural gene locus to 10q24→10q25

Cytogenetic and Genome Research ◽

10.1159/000130999 ◽

1978 ◽

Vol 22 (1-6) ◽

pp. 468-471 ◽

Cited By ~ 7

Author(s):

D.A. Aitken ◽

M.A. Ferguson-Smith

Keyword(s):

Structural Gene ◽

Gene Locus ◽

Gene Dosage ◽

Regional Assignment

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The Influence of Short Ether Anesthesia on Some Liver Enzyme Activities in the Rat

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y73-090 ◽

1973 ◽

Vol 51 (8) ◽

pp. 604-607 ◽

Cited By ~ 5

Author(s):

E. Katona

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Malate Dehydrogenase ◽

Enzyme Activities ◽

Urate Oxidase ◽

Arylsulfatase A ◽

Ether Anesthesia ◽

Arylsulfatase B ◽

Thiamine Pyrophosphatase ◽

Liver Homogenates

Rats were anesthetized by ether inhalation for 4–5 min and sacrificed 1–48 h after anesthesia. From their liver homogenates, the activities of nine enzymes were determined. Activities of urate oxidase and arylsulfatase-A did not change significantly but arylsulfatase-B was slightly decreased. Malate dehydrogenase, arylsulfatase-B, and thiamine pyrophosphatase reached their highest and "malic enzymes" their lowest activities at the same time, 5 h after anesthesia. Alkaline phosphatase first decreased, later increased. Acid phosphatase and glucose-6-phosphatase activities decreased following ether anesthesia. Thesechanges in the enzyme activities generally agree and partly explain previously reported effects of ether anesthesia observed in the serum.

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The effect of 2,4-D and kinetin on the activity and isoenzyme pattern of various enzymes in cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

Canadian Journal of Botany ◽

10.1139/b78-262 ◽

1978 ◽

Vol 56 (18) ◽

pp. 2185-2195 ◽

Cited By ~ 4

Author(s):

Paul G. Arnison ◽

W. G. Boll

Keyword(s):

Acid Phosphatase ◽

Phaseolus Vulgaris ◽

Cell Suspension ◽

Malate Dehydrogenase ◽

Leucine Aminopeptidase ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Glutamate Oxaloacetate Transaminase ◽

Cotyledon Cell ◽

Isoenzyme Patterns

Changes in the activity and isoenzyme patterns of acid phosphatase, leucine aminopeptidase, glutamate-oxaloacetate transaminase, esterase, and malate and glutamate dehydrogenases were studied in cotyledon cell suspension cultures of Phaseolus vulgaris grown in the presence and absence of the growth regulators 2,4-dichlorophenoxyacetic acid and kinetin. With all enzymes studied, the pattern of isoenzymes and total enzymatic activity changed with the different phases of the culture cycle. In particular, the patterns of esterase, malate dehydrogenase, and glutamate dehydrogenase changed markedly with the inoculation of cells into fresh medium.The differences in isoenzyme patterns of cells grown with and without regulators were predominantly quantitative. However, certain minor isoenzymes of acid phosphatase, glutamate-oxaloacetate transaminase, esterase, and malate dehydrogenase were only detected in cultures grown in the presence of the regulators, while one isoenzyme of leucine aminopeptidase and two of esterase were unique to cells cultured in the absence of regulators.Three cathodic isoenzymes of acid phosphatase were released from wall material by 1 M NaCl. Such isoenzymes were also detected in the medium and in cytoplasmic extracts. Increase in the wall isoenzymes following inoculation into fresh medium was correlated with a decrease in anodic, cytoplasmic acid phosphatase.

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