It has been verified that serum N-acetyl-?-D-glucosaminidase (NAG) activity
is elevated in diabetes, but there are no reports about changes of the sialic
acid (SA) content in the carbohydrate parts of NAG A form and its influence
on total NAG activity changes in type 1 diabetes mellitus patients without
and with secondary complications. NAG A forms were isolated, purified and
characterized from the serum of 81 IDDM patients with and without secondary
complications (retinopathy, polyneuropathy and nephropathy) and 25 healthy
persons. The content of ?-2,6-bound SA and isoenzyme patterns of purified A
form, total NAG and A form activities were determined. In all diabetic
groups, A form sialylation levels were 2-3.5 times lower compared to control,
while their acidities (fractions with pI 4.25-5.1) increased, particularly
with progression of secondary complications. Total serum NAG activities and
percentages of A form were significantly higher (P<0.001) in all diabetic
groups and negatively correlated with the ?-2,6-bound SA content of the A
form. In addition, they decreased as secondary diabetic complications became
more complex. Observed changes could be the consequence of structural changes
in the A form due to significant increase in its acidity, i.e. negative
charge which originate from groups other than SA.