Inheritance of isozymes in seed and bud tissues of blue and Engelmann spruce

Genome ◽  
1987 ◽  
Vol 29 (2) ◽  
pp. 239-246 ◽  
Author(s):  
S. G. Ernst ◽  
D. E. Keathley ◽  
J. W. Hanover
Keyword(s):  
Acid Phosphatase ◽  
Key Words ◽  
Malate Dehydrogenase ◽  
Glutamate Oxaloacetate Transaminase ◽  
Blue Spruce ◽  
Mating Design ◽  
Engelmann Spruce ◽  
Enzyme Systems ◽  
Segregation Ratios ◽  
Mode Of Inheritance

Thirteen loci from 11 enzyme systems were identified among full-sib and half-sib progeny of blue and Engelmann spruce. Eleven of the loci were expressed in bud, embryo, and megagametophyte tissue; the remaining two loci were expressed only in embryo and megagametophyte tissue. There were no mobility differences observed between loci expressed in seed and bud tissues. The mode of inheritance for 10 of the loci was confirmed based on progeny genotypic distributions. For the two loci not expressed in bud tissue, acid phosphatase (Acp-2) and diaphorase (Dia-2), inheritance was inferred from pooled segregation ratios of megagametophytes from open-pollinated seed from heterozygous females. The inheritance of glutamate oxaloacetate transaminase (Got-3) was also inferred from segregation ratios and diploid embryo phenotypes of open-pollinated progeny owing to a lack of variability at this locus among the 40 parents in the mating design. Two loci, aldolase (Ald) and malate dehydrogenase (Mdh-2), were monomorphic among the 20 parents of both species. Key words: isozymes, Engelmann spruce, blue spruce, Picea.

scholarly journals Studies on the genetic diversity of pointed gourd using biochemical methods (Isozyme analysis)

1970 ◽  
Vol 34 (1) ◽  
pp. 123-141
Author(s):  
ASMMR Khan ◽  
MG Rabbani ◽  
MA Siddique ◽  
MA Islam
Keyword(s):  
Genetic Diversity ◽  
Acid Phosphatase ◽  
Key Words ◽  
Glutamate Oxaloacetate Transaminase ◽  
Banding Patterns ◽  
Biochemical Methods ◽  
Electrophoretic Patterns ◽  
Wide Range ◽  
Rf Values ◽  
Pointed Gourd

Biochemical characterizations of 64 pointedgourds were done using three isozyme viz., acid phosphatase, peroxidase and glutamate oxaloacetate transaminase. A wide range of diversity among the gremplasm based on their acid phosphatase, peroxidase and glutamate oxaloacetate transaminase isoenzyme banding patterns were observed. In respect of isoenzyme activity; 8 acid phosphatase, 7 peroxidase and 9 glutamate oxaloacetate transminase electrophoretic zymotypes were formed by 19, 11, and 19 bands at different Rf values varying from 0.19 to 0.82, 0.38 to 0.69 and 0.15 to 0.95, respectively. The wide range of similarity co-efficient of 0.0-80.0, 0.0-66.0, and 0.0-80.0 as found among the electrophoretic patterns in acid phosphatase, peroxidase, and glutamate oxaloacetate transminase, respectively, indicating wide genetic diversity among the accessions. Based on the polymorphic activity of these three enzymes, 27 combinations of electrophoretic zymotypes were identified, each of which can he equated to genotypes. Each of the groups consisted of one to eight genotypes. Sixty four accessions of pointed gourd were grouped into 12 clusters. The genotypes collected from the same location were grouped into different clusters. Key Words: Genetic diversity; pointed gourd; biochemical methods. DOI: 10.3329/bjar.v34i1.5762Bangladesh J. Agril. Res. 34(1) : 123-141, March 2009

The effect of 2,4-D and kinetin on the activity and isoenzyme pattern of various enzymes in cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1978 ◽  
Vol 56 (18) ◽  
pp. 2185-2195 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Acid Phosphatase ◽  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Malate Dehydrogenase ◽  
Leucine Aminopeptidase ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Glutamate Oxaloacetate Transaminase ◽  
Cotyledon Cell ◽  
Isoenzyme Patterns

Changes in the activity and isoenzyme patterns of acid phosphatase, leucine aminopeptidase, glutamate-oxaloacetate transaminase, esterase, and malate and glutamate dehydrogenases were studied in cotyledon cell suspension cultures of Phaseolus vulgaris grown in the presence and absence of the growth regulators 2,4-dichlorophenoxyacetic acid and kinetin. With all enzymes studied, the pattern of isoenzymes and total enzymatic activity changed with the different phases of the culture cycle. In particular, the patterns of esterase, malate dehydrogenase, and glutamate dehydrogenase changed markedly with the inoculation of cells into fresh medium.The differences in isoenzyme patterns of cells grown with and without regulators were predominantly quantitative. However, certain minor isoenzymes of acid phosphatase, glutamate-oxaloacetate transaminase, esterase, and malate dehydrogenase were only detected in cultures grown in the presence of the regulators, while one isoenzyme of leucine aminopeptidase and two of esterase were unique to cells cultured in the absence of regulators.Three cathodic isoenzymes of acid phosphatase were released from wall material by 1 M NaCl. Such isoenzymes were also detected in the medium and in cytoplasmic extracts. Increase in the wall isoenzymes following inoculation into fresh medium was correlated with a decrease in anodic, cytoplasmic acid phosphatase.

Comparison of human and murine isolates of Schistosoma mansoni from Richard-Toll, Senegal, by isoelectric focusing

1997 ◽  
Vol 71 (2) ◽  
pp. 175-181 ◽  
Author(s):  
M. Sène ◽  
P. Brémond ◽  
J.P. Hervé ◽  
V.R. Southgate ◽  
B. Sellin ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Schistosoma Mansoni ◽  
Isoelectric Focusing ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Arvicanthis Niloticus

AbstractStudies on human and murine isolates of Schistosoma mansoni, from Richard-Toll, Senegal, were carried out by isoelectric focusing in polyacrylamide gels. Seven enzyme systems; lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), acid phosphatase (AcP), hexokinase (HK), glucose phosphate isomerase (GPI), and phosphoglucomutase (PGM), were used to compare the two isolates. All systems tested, apart from LDH, were found to be polymorphic for both isolates. Interestingly, one phenotype is more frequent than the remainder. The results show that there is no significant genetic variation between the S. mansoni isolates from man and the rodents, Arvicanthis niloticus and Mastomys huberti.

scholarly journals GENETICS OF ISOZYMES AND ANALYSIS OF ISOZYMES LINKAGE AND MORPHOLOGICAL LOCI IN SUNFLOWER (Helianthus annuus L.) / GENETICA DE ISOFERMENTOS Y EL ACOMPLAMIENTO DE LÓCUSES MORFOLOGICOS E ISOFERMENTICOS EN GIRASOL / GENETIQUE DES ISOFERMENTS ET LIAISON DES LOCUS MORPHOLOGIQUES ET CEUX-CI D’ISOFERMENTS AU TOURNESOL

Helia ◽  
2000 ◽  
Vol 23 (33) ◽  
pp. 65-76
Author(s):  
V.V. Kirichenko ◽  
V.N. Popov
Keyword(s):  
Acid Phosphatase ◽  
Linkage Group ◽  
Helianthus Annuus ◽  
Malate Dehydrogenase ◽  
Morphological Traits ◽  
Fertility Restoration ◽  
Linkage Groups ◽  
Esterase Loci ◽  
Mature Seeds ◽  
Male Fertility Restoration

SUMMARY The genetics of anodal esterase (Est), cathodal esterase (cEst), cathodal acid phosphatase (cAcp) and malate dehydrogenase (Mdh) has been studied in mature seeds and leaves (genetics of cAcp and Mdh has not been studied in leaves) of sunflower (Helianthus annuus L.). A total of ten loci (four loci of anodal esterase, two loci of cathodal esterase, three loci of malate dehydrogenase and one locus of cathodal acid phosphatase) have been identified and described. Five esterase loci (Est1, Est2, Est3, Est4, cEst5), three malate dehydrogenase loci and one locus of cathodal acid phosphatase are expressed in seeds. Three esterase loci (Est2, cEst5 and cEst6) are expressed in leaves. The analysis of linkage between these loci has been made. Two linkage groups have been found. The sequence of the loci in the first linkage group was Mdh2-Est1- Est2-Est3-cEst5. In the second linkage group it was Est4-cAcp1. Linkages have been analyzed between three isoenzymatic loci expressed in leaves and between two loci controlling morphological traits (branched stem and male fertility restoration). The linkage between morphological traits and isoenzymatic loci has not been revealed. It has been revealed in Br-Rf pair.

Activities of enzymes in β-carboxylation reactions and of catalase in cell-free preparations from the symbiotic dinoflagellates Symbiodinium spp. from a coral, a clam, a zoanthid and two sea anemones

1986 ◽  
Vol 228 (1253) ◽  
pp. 483-492 ◽  
Keyword(s):  
Malate Dehydrogenase ◽  
Sea Anemones ◽  
Stony Coral ◽  
Enzyme Systems ◽  
Glycollate Dehydrogenase ◽  
Order Of Magnitude ◽  
Symbiotic Dinoflagellates ◽  
Tridacna Maxima ◽  
First Time ◽  
Different Levels

Cell-free extracts of cultured and freshly isolated symbiotic dinoflagellates, Symbiodinium spp, isolated from the stony coral Montipora verrucosa , the clam Tridacna maxima , the zoanthid Palythoa sp. and the sea anemone Aiptasia pulchella were assayed for the enzyme systems involved in β-carboxylation and photorespiration. Markedly different levels of phosphoenolpyruvate carboxylase (EC 4 . 1 . 1 .31; PEP-case) activity were demonstrated in extracts from the different algae. The activity of PEP-case in the algae from M. verrucosa was highest, being an order of magnitude higher than PEP-case in algae from Palythoa sp. and up to 25-fold higher than that in algae from A. pulchella and T. maxima . The algae from M. verrucosa also exhibited pyruvate-P i dikinase (EC 2 . 7 .9 .1) activity. When these data are combined with previous demonstrations of the existence of NAD malate dehydrogenase and NADP malate dehydrogenase (decarboxylating; ‘malic enzyme’) in these algae, the indications are that they possess functional β-carboxylation enzyme systems. Past demonstrations of the photoassimilation of 14 CO 2 into glycollate by Symbiodinium spp. indicated photorespiration. The demonstration for the first time of activity of catalase (EC 1 . 11 . 1 . 6) when viewed in light of previous demonstrations of other composite enzymes of the photorespiratory pathway (e.g. phosphoglycollate phosphatase, glycollate dehydrogenase), add further confirmation to the disputed existence of a functional photorespiratory system in these symbiotic dinoflagellates.

ALBINISM AND NATURAL SELFING IN Picea pungens

1983 ◽  
Vol 63 (4) ◽  
pp. 1097-1098
Author(s):  
W. H. CRAM
Keyword(s):  
Key Words ◽  
Recessive Gene ◽  
Blue Spruce ◽  
Picea Pungens ◽  
Self Pollination

Albino seedlings from self-pollinated seed were attributed to a simple and lethal recessive gene, which was utilized to estimate the occurrence of 0–18% natural self-pollination in blue spruce (Picea pungens Engelm.).Key words: Albinism, natural selfing, Picea pungens

Inheritance of hull pubescence and seed color in annual canarygrass

2003 ◽  
Vol 83 (3) ◽  
pp. 471-474 ◽  
Author(s):  
M. A. Matus-Cádiz ◽  
P. Hucl ◽  
A. Vandenberg
Keyword(s):  
Key Words ◽  
Growth Stage ◽  
Seed Color ◽  
Breeding Line ◽  
Food Crop ◽  
Yellow Seed ◽  
Segregation Ratios ◽  
Recessive Genes ◽  
Hybrid Cross ◽  
Phalaris Canariensis

The availability of glabrous-hulled annual canarygrass (Phalaris canariensis L.) cultivars with yellow seed color may pave the way for developing this species into a food crop. The objective of this research was to study the inheritance of hull pubescence and seed color in annual canarygrass. A gametocide was applied to plants at Zadoks Growth Stage 42 to induce male sterility. CDC Maria, a glabrous-hulled and brown-seeded cultivar, was crossed with six pubescent-hulled, brownseeded annual canarygrass accessions and with CY193, a pubescent-hulled and yellow-seeded breeding line. In mono-hybrid crosses, segregation ratios of F2 populations were not significantly different from the phenotypic ratios of 3 pubescent-hulled: 1 glabrous-hulled for hull pubescence and 3 brown seeded: 1 yellow seeded for seed color. In the di-hybrid cross, a phenotypic ratio of 9 pubescent-hulled/brown seeded: 3 pubescent-hulled/yellow seeded: 3 glabrous-hulled/brown seeded: 1 glabrous-hulled/yellow seeded was observed. Glabrous-hulled and yellow seeded traits are each controlled by single recessive genes that segregate independently in annual canarygrass. Key words: Phalaris canariensis, canaryseed, inheritance, hull pubescence, seed color

Vegetation development on skid trails and burned sites in southeastern British Columbia

1993 ◽  
Vol 69 (1) ◽  
pp. 75-80 ◽  
Author(s):  
E. T. Oswald ◽  
B. N. Brown
Keyword(s):  
British Columbia ◽  
Key Words ◽  
Tree Growth ◽  
Burned Area ◽  
Tree Seedlings ◽  
Vegetation Development ◽  
Engelmann Spruce ◽  
Forest Region ◽  
Invading Species ◽  
Study Sites

Vegetation development, including both planted and natural tree seedlings, was examined over a 5-year period on an area in the ESSFmw subzone of the Nelson Forest Region which was clearcut, skid logged, burned, and planted with 2 + 0, 313, Engelmann spruce. Study sites included the cutbank, mid-trail, and sidecast portions of the skid trails and the intervening prescribed burned area. Fireweed was the most prominent invading species, and affected tree growth, particularly on the sidecast, by shading and crowding the trees. Black huckleberry, regenerating from root stalks, was common on the burned sites, but had marginal influence on Engelmann spruce development over 5 years. The best tree growth occurred on the burned sites, followed by the sidecast, mid-trail, and cutbank sites. Consideration should be given to omitting the cutbank planting site and moving the mid-trail planting site inward on slopes of 30% or more. Key words: Engelmann spruce, ESSFmw biogeoclimatic subzone, slash burn, vegetation succession, fireweed

scholarly journals The kinetics of enzyme changes in yeast under conditions that cause the loss of mitochondria

1968 ◽  
Vol 107 (4) ◽  
pp. 455-465 ◽  
Author(s):  
C. Chapman ◽  
W Bartley
Keyword(s):  
Cytochrome C ◽  
Glutamate Dehydrogenase ◽  
Malate Dehydrogenase ◽  
Nadh Oxidase ◽  
Isocitrate Lyase ◽  
Lag Phase ◽  
Glutamate Oxaloacetate Transaminase ◽  
Oxoglutarate Dehydrogenase ◽  
Nadh Cytochrome ◽  
Glucose 6 Phosphate Dehydrogenase

1. Aerobically grown yeast having a high activity of glyoxylate-cycle, citric acid-cycle and electron-transport enzymes was transferred to a medium containing 10% glucose. After a lag phase of 30min. the yeast grew exponentially with a mean generation time of 94min. 2. The enzymes malate dehydrogenase, isocitrate lyase, succinate–cytochrome c oxidoreductase and NADH–cytochrome c oxidoreductase lost 45%, 17%, 27% and 46% of their activity respectively during the lag phase. 3. When growth commenced pyruvate kinase, pyruvate decarboxylase, alcohol dehydrogenase, glutamate dehydrogenase (NADP+-linked) and NADPH–cytochrome c oxidoreductase increased in activity, whereas aconitase, isocitrate dehydrogenase (NAD+- and NADP+-linked), α-oxoglutarate dehydrogenase, fumarase, malate dehydrogenase, succinate–cytochrome c oxidoreductase, NADH–cytochrome c oxidoreductase, NADH oxidase, NADPH oxidase, cytochrome c oxidase, glutamate dehydrogenase (NAD+-linked), glutamate–oxaloacetate transaminase, isocitrate lyase and glucose 6-phosphate dehydrogenase decreased. 4. During the early stages of growth the loss of activity of aconitase, α-oxoglutarate dehydrogenase, fumarase and glucose 6-phosphate dehydrogenase could be accounted for by dilution by cell division. The lower rate of loss of activity of isocitrate dehydrogenase (NAD+- and NADP+-linked), glutamate dehydrogenase (NAD+-linked), glutamate–oxaloacetate transaminase, NADPH oxidase and cytochrome c oxidase implies their continued synthesis, whereas the higher rate of loss of activity of malate dehydrogenase, isocitrate lyase, succinate–cytochrome c oxidoreductase, NADH–cytochrome c oxidoreductase and NADH oxidase means that these enzymes were actively removed. 5. The mechanisms of selective removal of enzyme activity and the control of the residual metabolic pathways are discussed.

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