The effect of 2,4-D and kinetin on the activity and isoenzyme pattern of various enzymes in cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1978 ◽  
Vol 56 (18) ◽  
pp. 2185-2195 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Acid Phosphatase ◽  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Malate Dehydrogenase ◽  
Leucine Aminopeptidase ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Glutamate Oxaloacetate Transaminase ◽  
Cotyledon Cell ◽  
Isoenzyme Patterns

Changes in the activity and isoenzyme patterns of acid phosphatase, leucine aminopeptidase, glutamate-oxaloacetate transaminase, esterase, and malate and glutamate dehydrogenases were studied in cotyledon cell suspension cultures of Phaseolus vulgaris grown in the presence and absence of the growth regulators 2,4-dichlorophenoxyacetic acid and kinetin. With all enzymes studied, the pattern of isoenzymes and total enzymatic activity changed with the different phases of the culture cycle. In particular, the patterns of esterase, malate dehydrogenase, and glutamate dehydrogenase changed markedly with the inoculation of cells into fresh medium.The differences in isoenzyme patterns of cells grown with and without regulators were predominantly quantitative. However, certain minor isoenzymes of acid phosphatase, glutamate-oxaloacetate transaminase, esterase, and malate dehydrogenase were only detected in cultures grown in the presence of the regulators, while one isoenzyme of leucine aminopeptidase and two of esterase were unique to cells cultured in the absence of regulators.Three cathodic isoenzymes of acid phosphatase were released from wall material by 1 M NaCl. Such isoenzymes were also detected in the medium and in cytoplasmic extracts. Increase in the wall isoenzymes following inoculation into fresh medium was correlated with a decrease in anodic, cytoplasmic acid phosphatase.

The effect of 2,4-D and kinetin on peroxidase activity and isoenzyme pattern in cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1976 ◽  
Vol 54 (16) ◽  
pp. 1857-1867 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Growth Regulators ◽  
Peroxidase Activity ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Peroxidase Isoenzymes ◽  
Cotyledon Cell ◽  
Cell Growth And Differentiation ◽  
Dichlorophenoxy Acetic Acid

Cotyledon cell suspension cultures of Phaseolus vulgaris were grown in the presence and absence of the growth regulators (2,4-dichlorophenoxy)acetic acid (2,4-D) and kinetin. Peroxidase (EC 1.11.1.7) activity was at a minimum during the phase of cell division and at a maximum during the phase of cell expansion. Both the pattern and activity of peroxidase isoenzymes changed during the culture cycle.Cells cultured without growth regulators showed increased peroxidase activity and changed isoenzyme patterns. Certain peroxidase isoenzymes were only present or prominent during specific phases of the culture cycle.The electrophoretic mobilities of peroxidase isoenzymes detected in the medium were not the same as those of the cytoplasmic isoenzymes. Cell cultures grown with and without growth regulators showed different patterns of medium peroxidase activity.Results are discussed in relation to the correlation of peroxidase activity with cell wall expansion and the possible role of peroxidase in cell growth and differentiation.

The effect of 2,4-D and kinetin on the morphology, growth, and cytochemistry of peroxidase of cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1976 ◽  
Vol 54 (16) ◽  
pp. 1847-1856 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Peroxidase Activity ◽  
Morphological Characteristics ◽  
Enzyme Reaction ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Age ◽  
Cotyledon Cell ◽  
Dichlorophenoxy Acetic Acid

Cotyledon cell suspension cultures of Phaseolus vulgaris were grown in the presence and absence of the growth regulators (2,4-dichlorophenoxy)acetic acid (2,4-D) and kinetin. Omission of the regulators changed the growth and cell division and produced striking changes in morphological characteristics. Cytochemical studies of peroxidase activity showed that the enzymes were mainly cytoplasmic in young cells and mainly associated with wall in older cells. No apparent differences in localization of activity were detected between the treatments, but cells cultured without the regulators showed a much more intense enzyme reaction. The location and increase of peroxidase, with increasing cell age and cell complexity, are consistent with the view that peroxidase activity is involved in cell wall expansion and differentiation.

Isoenzymes in cell cultures of bush bean (Phaseolus vulgaris cv. Contender): isoenzymatic differences between stock suspension cultures derived from a single seedling

1975 ◽  
Vol 53 (3) ◽  
pp. 261-271 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Acid Phosphatase ◽  
Phaseolus Vulgaris ◽  
Cell Cultures ◽  
Suspension Cultures ◽  
Peroxidase Isoenzymes ◽  
Bush Bean ◽  
Leucine Amino Peptidase ◽  
The Media ◽  
Isoenzyme Patterns ◽  
Stock Suspension

Electrophoretic analyses of isoenzyme patterns were performed with extracts of root, hypocotyl, and cotyledon suspension cultures derived from a single seedling. The enzymes studied included malate, glutamate, and glucose-6-phosphate dehydrogenases; peroxidase; polyphenol oxidase; esterase; acid phosphatase; and leucine amino peptidase. Peroxidase isoenzymes were also detected in the media. The isoenzymatic patterns of the three cultures were different for some enzymes, similar for others, and identical for the rest. The isoenzymatic patterns were recorded on a number of occasions over a period of 3 years and they remained relatively unchanged.

scholarly journals 31P NMR Study of Elicitor Treated Phaseolus vulgaris Cell Suspension Cultures

1987 ◽  
Vol 85 (3) ◽  
pp. 716-719 ◽  
Author(s):  
Israel Ojalvo ◽  
J. Stefan Rokem ◽  
Gil Navon ◽  
Israel Goldberg
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
31P Nmr ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Nmr Study

scholarly journals Sucrose metabolizing enzymes in cell suspension cultures of Bauhinia forficata, Curcuma zedoaria and Phaseolus vulgaris

2001 ◽  
Vol 36 (9) ◽  
pp. 1085-1092 ◽  
Author(s):  
Marcia Ometto de Mello ◽  
Antônio Francisco de Campos Amaral ◽  
Murilo Melo
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Sucrose Synthase ◽  
Sucrose Concentration ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Curcuma Zedoaria ◽  
Metabolizing Enzymes ◽  
Sucrose Metabolizing Enzymes ◽  
Bauhinia Forficata

The objective of this work was to study the activity of sucrose metabolizing enzymes in extracts of cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. Invertase pathway was identified in the three studied species. Sucrose synthase pathway was also responsible for sucrose metabolism in Curcuma zedoaria and Phaseolus vulgaris cells. Activity values higher than 300 nmol min-1 mg-1 of protein were found for acid and neutral invertases, UDPglucose pyrophosphorylase and phosphoglucomutase in the cell extract of the three plant species. Sucrose synthase showed low activity in Bauhinia forficata cells. As sucrose concentration in the culture medium decreased, sucrose synthase activity increased in C. zedoaria and P. vulgaris cells. The glycolytic enzymes activity gradually reduced at the end of the culture period, when carbohydrate was limited.

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