Enhanced symbiotic seed germination of Cypripedium macranthos var. rebunense following inoculation after cold treatment

<p style="text-align: justify;">Using the« standard» germination method (dehydration, hydration before conserving at 5°C during several weeks or several months and rehydration followed by the germination at 27°C/28°C), it is possible to conserve grape seeds of <em>Vitis vinifera</em> c.v. Ugni blanc more than one year and preserve a high ability of germination. However, to be able to work on young seedlings rapidly after harvesting, this method requiring a too long breaking dormancy treatment (conserving at 5°C), we tried to change these treatments and to speed up the breaking dormancy using an anoxic treatment finalized at the laboratory. Even if this system does not allow to obtain the best rate of germination, it aIlows to save 50 days for Ugni blanc treatment and more than 150 days for Merlot avoiding breaking dormancy with cold treatment. To improve the grape seed ability of germination following the « standard» method, we used a solution of etephon in external supply, releasing ethylene in tissues. Seeds conserved 90 days at 5°C and hydrated with this solution have a better rate of germination (+ 10 p. cent in comparison with a standard sample treated with the same method without ethylene). Finally, non conserved at 5°C grape seed lyophilization can be a rapid method for breaking dormancy and improving (at least 40 p. cent) the ability of germination.</p>

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Seed production and seed germination of Amsinckia hispida

Australian Journal of Experimental Agriculture ◽

10.1071/ea9650495 ◽

1965 ◽

Vol 5 (19) ◽

pp. 495 ◽

Cited By ~ 4

Author(s):

DJ Connor

Keyword(s):

Seed Germination ◽

Environmental Factors ◽

Seed Production ◽

Constant Temperature ◽

Cold Treatment ◽

North West ◽

Seed Maturity ◽

Germination Behaviour ◽

Western Victoria ◽

North Western

Amsinckia hispida is a serious annual weed of cereal culture in north-western Victoria. Individual plants produce seed for up to two months in the spring and this provides a range of seed maturity that is reflected by a gradual build-up of germinability in the following autumn. In addition the seed has an optimum constant temperature for germination of 13�C (48�F) and responds markedly to a period of cold treatment. These three features act together to produce the series of Amsinckia populations which are characteristic of its autumn germination behaviour in north-west Victoria. Seeds which germinate early are responsible for infestations in the pasture phase and those which germinate later are not controlled by pre-sowing cultivation and remain to germinate within crops. The response of Amsinckia to environmental factors is such that these plants which germinate late within wheat crops have an extended rosette period and are highly competitive.

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Effects of mycorrhizal fungi on symbiotic seed germination of Pecteilis susannae (L.) Rafin (Orchidaceae), a terrestrial orchid in Thailand

Symbiosis ◽

10.1007/s13199-011-0120-8 ◽

2011 ◽

Vol 53 (3) ◽

pp. 149-156 ◽

Cited By ~ 11

Author(s):

Ruangwut Chutima ◽

Bernard Dell ◽

Saisamorn Lumyong

Keyword(s):

Seed Germination ◽

Mycorrhizal Fungi ◽

Terrestrial Orchid ◽

Pecteilis Susannae ◽

Symbiotic Seed Germination

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In Vitro Decoated Seed Germination and Seedling Development for Propagation of Wild Mandrake (Mandragora autumnalis Bertol.)

Plants ◽

10.3390/plants9101339 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1339

Author(s):

Hani Al-Ahmad

Keyword(s):

Seed Germination ◽

Zygotic Embryo ◽

Seedling Emergence ◽

Cold Treatment ◽

Fresh Weight ◽

Plant Materials ◽

System P ◽

Double Number

The establishment of an efficient in vitro propagation system for the conservation of the Mediterranean Mandragora autumnalis is highly desirable due to its scarcity, besides its potential medicinal and pharmacological properties. In a separate unpublished study, this species has proved to be resistant to laboratory plant regeneration from vegetative tissue cultures; therefore, an alternative decoated seed (i.e., endosperm enclosed the zygotic embryo) germination approach was conducted in this study. Pre-cold treatment of M. autumnalis seeds, removal of seed coats, and exogenous application of gibberellic acid (GA3) promoted in vitro seed germination and seedling emergence. In two separate experiments, approximately 10–27% of the germinated decoated seeds developed healthy seedlings within two weeks, compared to the non-germinated intact seeds of the potting soil controls. After 72 days, the highest rates of healthy seedlings development (67.4 and 69.4%) achieved in the in vitro decoated seed cultures supplemented with 60 and 100 mg/L GA3, respectively, compared to only 25% seedlings emergence rate of the in vitro cultures devoid of GA3, and 44.2% of the soil controls. The in vitro developed plants were healthy, survived transplantation conditions, and, significantly, grew faster, formed on average more than the double number of true leaves and shoot fresh weight (p ≤ 0.05), 90% more fresh weight of root system (p ≤ 0.05), and ultimately more than the double gross fresh weight (p ≤ 0.05) than that of the in vivo developed plants of the soil controls. Such in vitro seed germination approaches would be favorable due to the higher capacity of uniform seedling establishment year-round under lab-controlled conditions, facilitating proliferation and conservation of rare and threatened species, and providing fresh and axenic plant materials required for downstream studies such as those associated with leaf-derived protoplasts and genetic transformations.

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