Fungi isolated from host protocorms accelerate symbiotic seed germination in an endangered orchid species (Dendrobium chrysotoxum) from southern China

Mycorrhiza ◽  
2020 ◽  
Vol 30 (4) ◽  
pp. 529-539 ◽  
Author(s):  
Shi-Cheng Shao ◽  
Qiu-Xia Wang ◽  
Kingly C. Beng ◽  
Da-Ke Zhao ◽  
Hans Jacquemyn
2000 ◽  
Vol 43 (3) ◽  
pp. 158-161 ◽  
Author(s):  
So Young Park ◽  
Hosakatte N Murthy ◽  
Kee Yoeup Paek

Lankesteriana ◽  
2015 ◽  
Vol 7 (1-2) ◽  
Author(s):  
Emily Massey ◽  
Lawrence Zettler

Interest in using mycorrhizal fungi to cultivate orchids from seed in vitro (=symbiotic seed germina- tion) has intensified in recent years and this approach is now an important conservation tool worldwide. In North America, symbiotic germination has been attempted for a growing number of orchid species in peril as a means to acquire seedlings suitable for reintroduction.


2001 ◽  
Vol 71 (2) ◽  
pp. 585 ◽  
Author(s):  
J. Ochora ◽  
W. D. Stock ◽  
H. P. Linder ◽  
L. E. Newton

2017 ◽  
Vol 58 (1) ◽  
Author(s):  
Xu Zeng ◽  
Yuanyuan Li ◽  
Hong Ling ◽  
Sisi Liu ◽  
Mengmeng Liu ◽  
...  

2015 ◽  
Vol 3 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Varsha Dawande ◽  
Rajaram Gurav

Asymbiotic seed germination of Eulophia nuda Lindl. was observed on Knudson C medium. About 90% seeds germinated within 8-10 weeks and formed green protocorms in 11-12 weeks. Effect of BA and IBA was studied on plantlet development from protocorms. BA shows the best results with respect to number and length of shoots. Maximum number (6.45±1.36) and length (3.90±0.99) was observed on MS medium supplemented with 4.44μM.BA. Maximum root growth was also observed on same medium (4.8±0.99 number of roots and 1.43±0.13cm length). The regenerated plantlets were successfully acclimatized and transferred to earthen pots. The results presented here show that in vitro seed germination and plantlet development in Eulophia nuda Lindl., an endangered orchid, can be achieved at a higher rate by this method.Int J Appl Sci Biotechnol, Vol 3(2): 243-247 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12476   


2016 ◽  
Vol 2 (3) ◽  
pp. 22-28 ◽  
Author(s):  
Syifaur Rahmah ◽  
Ni Made Armini Wiendi ◽  
Willy B. Suwarno ◽  
Krisantini Krisantini

Dendrobium mannii and Dendrobium mirbelianum were rare and endangered orchid species. In vitro micro propagation could be used to conserve and proliferate these species. The aims of this study were to determine the effects of chitosan on proliferation of the protocorm-like-bodies (PLBs) of in vitro grown Dendrobium mannii and D. mirbelianum. Chitosan has been reported as an important component to promote orchid growth in vitro. In this study chitosan was included in the in-vitro media at the concentrations of 0, 1, 2, 3, and 4 mg.L-1. The highest PLBs formation, clump diameter, and fresh weight on D. mannii were obtained from explants cultured on medium supplemented with 1 mg.L-1 chitosan. After 14 weeks of culture, fresh weight, clump diameter and average number of PLBs of D.mannii were 29.52 %, 33.00 % and 35.88% greater at chitosan (1 mg.L-1) compared to control media.  D. mirbelianum treated with low chitosan concentrations (1 and 2 mg.L-1) had 65.71% more leaves than those without chitosan treatment. Chitosan, however, had an adverse effects on growth of both species at  4 mg.L-1. Medium contain 4 mg.L-1 chitosan induced PLBs proliferation of D.mannii, but inhibited PLBs proliferation of D.mirbelianum.  These results suggested that chitosan had important roles in PLB proliferation and could be used for the propagation of D. mannii PLBs in vitro. Keywords: rare orchid species, in-vitro


2021 ◽  
Vol 42 ◽  
pp. e67589
Author(s):  
Daniela Alba-Patiño ◽  
Fabian Martínez-Hernández ◽  
Juan Francisco Mota Poveda

Colombia is the country with the highest number of orchid species (4270), whose optimal habitat is cold and humid forests. However, the outlook for conservation is alarming, considering that deforestation is causing the loss of millions of hectares of forests. This situation has led to the existence of 206 endangered orchid species. Therefore, this research was conducted to determine Sites of Special Importance for the Conservation of Threatened Orchid Species in Colombia (SSICO), through an analysis of their spatial and altitudinal distribution using various databases, to make a selection of nature reserves on a municipality scale, using Marxan software, and employing relevant parameters (richness, rarity, and IUCN category). Furthermore, the results were later compared with the Protected Areas System, determining their coverage to propose SSICOs. 674 records of the presence of threatened orchids in 277 municipalities were obtained. Urrao, Abrego, and Frontino were the areas with the greatest richness and rarity. Marxan selected 47 municipalities located mostly in the Andes region, and four SSICOs were prioritized, which are located in the Antioquia, Norte de Santander, Nariño and Putumayo provinces. These SSICOs, in addition to being points of great biodiversity, are areas with special socio-economic characteristics that influence the management of natural resources. These areas require timely attention, research, and intervention by environmental authorities because of their importance for conserving orchids and Andes Forests.


2018 ◽  
Vol 3 (1) ◽  
pp. 225-226
Author(s):  
Seon A. Yun ◽  
Hyun-Deok Son ◽  
Hyoung-Tak Im ◽  
Seung-Chul Kim

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