scholarly journals Integrative Suppression of Temperature-Sensitive Mutants with a Lesion in the Initiation of DNA Replication. Replication of Autonomous Plasmids in the Suppressed State

1974 ◽  
Vol 43 (1) ◽  
pp. 125-130 ◽  
Author(s):  
Werner Goebel
Keyword(s):  
Dna Replication ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants ◽  
Initiation Of Dna Replication

New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication

1982 ◽  
Vol 187 (1) ◽  
pp. 42-46 ◽  
Author(s):  
Lawrence B. Dumas ◽  
Joan P. Lussky ◽  
Elizabeth J. McFarland ◽  
Janis Shampay
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Replication ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants

Genetic Analysis of Yeast RPA1 Reveals Its Multiple Functions in DNA Metabolism

Genetics ◽  
1998 ◽  
Vol 148 (3) ◽  
pp. 989-1005 ◽  
Author(s):  
Keiko Umezu ◽  
Neal Sugawara ◽  
Clark Chen ◽  
James E Haber ◽  
Richard D Kolodner
Keyword(s):  
Dna Replication ◽  
Dna Binding ◽  
Protein A ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Physical Analysis ◽  
Single Stranded Dna ◽  
Temperature Sensitive Mutants

Abstract Replication protein A (RPA) is a single-stranded DNA-binding protein identified as an essential factor for SV40 DNA replication in vitro. To understand the in vivo functions of RPA, we mutagenized the Saccharomyces cerevisiae RFA1 gene and identified 19 ultraviolet light (UV) irradiation- and methyl methane sulfonate (MMS)-sensitive mutants and 5 temperature-sensitive mutants. The UV- and MMS-sensitive mutants showed up to 104 to 105 times increased sensitivity to these agents. Some of the UV- and MMS-sensitive mutants were killed by an HO-induced double-strand break at MAT. Physical analysis of recombination in one UV- and MMS-sensitive rfa1 mutant demonstrated that it was defective for mating type switching and single-strand annealing recombination. Two temperature-sensitive mutants were characterized in detail, and at the restrictive temperature were found to have an arrest phenotype and DNA content indicative of incomplete DNA replication. DNA sequence analysis indicated that most of the mutations altered amino acids that were conserved between yeast, human, and Xenopus RPA1. Taken together, we conclude that RPA1 has multiple roles in vivo and functions in DNA replication, repair, and recombination, like the single-stranded DNA-binding proteins of bacteria and phages.

scholarly journals Control of Initiation of Sporulation by Replication Initiation Genes in Bacillus subtilis

2000 ◽  
Vol 182 (10) ◽  
pp. 2989-2991 ◽  
Author(s):  
Katherine P. Lemon ◽  
Iren Kurtser ◽  
Judy Wu ◽  
Alan D. Grossman
Keyword(s):  
Bacillus Subtilis ◽  
Dna Replication ◽  
Replication Initiation ◽  
Spore Formation ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutation ◽  
Initiation Of Dna Replication ◽  
Replication Initiation Proteins

ABSTRACT Initiation of spore formation in Bacillus subtilisappears to depend on initiation of DNA replication. This regulation was first identified using a temperature-sensitive mutation indnaB. We found that mutations in the replication initiation genes dnaA and dnaD also inhibit sporulation, indicating that inhibition of sporulation is triggered by general defects in the function of replication initiation proteins.

Apoptosis Was Promoted at a Nonpermissive Temperature in DNA Replication-Defective Temperature-Sensitive Mutants of Mouse FM3A Cells

1998 ◽  
Vol 238 (2) ◽  
pp. 317-323 ◽  
Author(s):  
Yukika Yamauchi ◽  
Akiko Tanaka ◽  
Kazunori Yamaguchi ◽  
Masayuki Kobayashi ◽  
Seiichi Shimamura ◽  
...  
Keyword(s):  
Dna Replication ◽  
Temperature Sensitive ◽  
Nonpermissive Temperature ◽  
Temperature Sensitive Mutants ◽  
Fm3a Cells

scholarly journals DNA polymerase I is required for premeiotic DNA replication and sporulation but not for X-ray repair in Saccharomyces cerevisiae

1989 ◽  
Vol 9 (2) ◽  
pp. 365-376
Author(s):  
M E Budd ◽  
K D Wittrup ◽  
J E Bailey ◽  
J L Campbell
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Replication ◽  
Dna Polymerase ◽  
Temperature Sensitive ◽  
Dna Polymerase I ◽  
X Ray ◽  
Temperature Sensitive Mutants ◽  
Polymerase I ◽  
Dna Polymerase Ii

We have used a set of seven temperature-sensitive mutants in the DNA polymerase I gene of Saccharomyces cerevisiae to investigate the role of DNA polymerase I in various aspects of DNA synthesis in vivo. Previously, we showed that DNA polymerase I is required for mitotic DNA replication. Here we extend our studies to several stages of meiosis and repair of X-ray-induced damage. We find that sporulation is blocked in all of the DNA polymerase temperature-sensitive mutants and that premeiotic DNA replication does not occur. Commitment to meiotic recombination is only 2% of wild-type levels. Thus, DNA polymerase I is essential for these steps. However, repair of X-ray-induced single-strand breaks is not defective in the DNA polymerase temperature-sensitive mutants, and DNA polymerase I is therefore not essential for repair of such lesions. These results suggest that DNA polymerase II or III or both, the two other nuclear yeast DNA polymerases for which roles have not yet been established, carry out repair in the absence of DNA polymerase I, but that DNA polymerase II and III cannot compensate for loss of DNA polymerase I in meiotic replication and recombination. These results do not, however, rule out essential roles for DNA polymerase II or III or both in addition to that for DNA polymerase I.

scholarly journals RCC1, a regulator of mitosis, is essential for DNA replication.

1992 ◽  
Vol 12 (8) ◽  
pp. 3337-3345 ◽  
Author(s):  
M Dasso ◽  
H Nishitani ◽  
S Kornbluth ◽  
T Nishimoto ◽  
J W Newport
Keyword(s):  
Dna Replication ◽  
Regulatory System ◽  
Sperm Chromatin ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Replication Complex ◽  
Temperature Sensitive Mutants ◽  
Egg Extracts ◽  
Xenopus Egg ◽  
Xenopus Egg Extracts

Temperature-sensitive mutants in the RCC1 gene of BHK cells fail to maintain a correct temporal order of the cell cycle and will prematurely condense their chromosomes and enter mitosis at the restrictive temperature without having completed S phase. We have used Xenopus egg extracts to investigate the role that RCC1 plays in interphase nuclear functions and how this role might contribute to the known phenotype of temperature-sensitive RCC1 mutants. By immunodepleting RCC1 protein from egg extracts, we find that it is required for neither chromatin decondensation nor nuclear formation but that it is absolutely required for the replication of added sperm chromatin DNA. Our results further suggest that RCC1 does not participate enzymatically in replication but may be part of a structural complex which is required for the formation or maintenance of the replication machinery. By disrupting the replication complex, the loss of RCC1 might lead directly to disruption of the regulatory system which prevents the initiation of mitosis before the completion of DNA replication.

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