Systematic application of high-resolution, phase-sensitive two-dimensional 1H-NMR techniques for the identification of the amino-acid-proton spin systems in proteins. Rabbit metallothionein-2

1985 ◽  
Vol 151 (2) ◽  
pp. 257-273 ◽  
Author(s):  
David NEUHAUS ◽  
Gerhard WAGNER ◽  
Milan VASAK ◽  
Jeremias H. R. KAGI ◽  
Kurt WUTHRICH
Keyword(s):  
Amino Acid ◽  
High Resolution ◽  
1H Nmr ◽  
Spin Systems ◽  
Proton Spin ◽  
Two Dimensional ◽  
Systematic Application ◽  
Acid Proton ◽  
Nmr Techniques ◽  
Phase Sensitive

Two-dimensional crystallization of membrane proteins

1992 ◽  
Vol 25 (1) ◽  
pp. 1-49 ◽  
Author(s):  
W. Kühlbrandt
Keyword(s):  
High Resolution ◽  
Membrane Proteins ◽  
Molecular Mass ◽  
Solution Nmr ◽  
Two Dimensional ◽  
Foreseeable Future ◽  
Nmr Techniques

In spite of several great breakthroughs, the overall rate of progress in determining high-resolution structures of membrane proteins has been slow. This is entirely due to the scarcity of suitable, well-ordered crystals. Most membrane proteins are multimeric complexes with a composite molecular mass in excess of 50000 Da which puts them outside the range of current solution NMR techniques. For the foreseeable future, detailed information about the structure of large membrane proteins will therefore depend on crystallographic methods.

scholarly journals A TRITERPENE ESTER SAPONIN FROM THE SEED OF Barringtonia asiatica

2010 ◽  
Vol 3 (3) ◽  
pp. 149-155 ◽  
Author(s):  
Rymond J Rumampuk ◽  
Emma J Pongoh ◽  
Ponis Tarigan ◽  
Anthony J Herlt ◽  
Lewis N Mander
Keyword(s):  
High Resolution ◽  
Two Dimensional ◽  
Nmr Techniques

A triterpene ester saponin has been isolated from the seed of Barringtonia asiatica and its structure has been established by extensive application of high-resolution FABMS and two dimensional NMR techniques to be 3-O-{[β-D-galactopyranosyl(1→3)-β-D-glucopyranosyl(1→2)]-β-D-glucuronopyranosyloxy}-22-O-[2-methylbutyroyloxy]-15,16,28-trihydroxy-(3β,15α,16α,22α)-olean-12-ene.   Keywords: Barringtonia asiatica, Lecythidaceae, triterpenoid

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