Evaluation of Pathogenicity and of Cultural and Biochemical Tests for Identification of Pseudomonas syringae Pathovars syringae, morsprunorum and persicae from Fruit Trees

1994 ◽  
Vol 141 (1) ◽  
pp. 59-76 ◽  
Author(s):  
A. Burkowicz ◽  
K. Rudolph
Keyword(s):  
Pseudomonas Syringae ◽  
Fruit Trees ◽  
Biochemical Tests ◽  
Pseudomonas Syringae Pathovars

scholarly journals Isolation and characterization of Pseudomonas syringae isolates affecting stone fruits and almond in Montenegro

2021 ◽  
Author(s):  
Tamara Popović ◽  
Jelena Menković ◽  
Anđelka Prokić ◽  
Nevena Zlatković ◽  
Aleksa Obradović
Keyword(s):  
Pseudomonas Syringae ◽  
Housekeeping Genes ◽  
Molecular Characteristics ◽  
Biochemical Tests ◽  
High Genetic Diversity ◽  
Leaf Petiole ◽  
Molecular Features ◽  
Isolation And Characterization ◽  
Genomic Species

AbstractIn Montenegro, stone fruit species are grown on intensive and semi-intensive commercial plantations. However, almond production is mainly organized on family gardens and for household consumption. During two seasons (2017–2018), we surveyed apricot, peach, nectarine, sweet cherry, Japanese plum, and almond orchards for the presence of bacterial diseases at different geographical locations in Montenegro. From leaf, petiole and fruit lesions, branch or twig cankers, and necrotizing buds, a total of 29 isolates were obtained and subjected to identification based on their morphological, pathogenic, biochemical, and molecular characteristics. Pathogenicity of the isolates was confirmed by reproducing the symptoms on leaves, fruits, and twigs of the corresponding host plants. The biochemical tests indicated that the isolates belong to Pseudomonas syringae. However, isolates’ characterization showed variation in their phenotypic and molecular features. The presence of the syrB gene and ice nucleation activity grouped most of the isolates within pathovar syringae. The results of rep-PCR using the BOX primer revealed high genetic diversity of isolates. Multilocus sequence analysis (MLSA), using four housekeeping genes, showed that 27 isolates belong to the genomic species 1, P. syringae sensu stricto, corresponding to P. syringae phylogroup 2. However, isolates from the same phylogroup 2 did not form a monophyletic group. One strain isolated from apricot was most distinct and similar to members of genomic species 2, phylogroup 3. All tested isolates showed significant levels of resistance to copper sulfate and high level of sensitivity to streptomycin sulfate in vitro.

Detection of Pseudomonas syringae pv. Savastanoi, causal agent of olive tuberculosis in two regions of Western Algeria (Ain Témouchent and Sig)

2019 ◽  
Vol 9 (2) ◽  
pp. 64-71
Author(s):  
Benyoub Kheira ◽  
Kacem Mourad ◽  
Kaid-Harche Meriem
Keyword(s):  
Pseudomonas Syringae ◽  
Olive Tree ◽  
Causal Agent ◽  
Biochemical Tests ◽  
Node Disease ◽  
Different Temperatures ◽  
Leaf Level ◽  
Western Algeria ◽  
Olive Trees ◽  
Tree Leaf

The present study on olive tuberculosis commenced by isolating bacteria of the genus Pseudomonas from the soils and necrosis of collected olive trees. A total of 180 samples were used in this study: (100) rhizospheric soil samples: (60) samples at the region of Ain Témouchent and (40) at the region of Sig in western of Algeria. In total, (80) galls were collected (40) at branch level and (40) galls at olive tree leaf (level). The isolates were identified by microbiological (macroscopic and microscopic examination), physiological (growth in the presence of Salt (NaCl), growth at different pH values and growth at different temperatures) and biochemical methods (the LOPAT and Galeries Api 20 NE test to identify species of the Pseudomonas group and conventional biochemical tests to identify the subspecies P. syringae pv. Savastanoi).This allowed to identify 110 isolates of Pseudomonas (60 isolates of P. aeruginosa, 35 isolates of P. fluorescens and 15 isolates of P. syringae pv Savastanoi the causal agent of olive node disease) which are now part of the collection of Pseudomonas bacteria of the laboratory of the Biotechnology Department (USTO-MB). The selection of technological performance isolates useful for our agriculture could solve phytopathological problems and finally constitute a collection of the bacteria preserved.

Distribution and Characterisation of vir Gene Homologues in Pseudomonas syringae Pathovars

2001 ◽  
pp. 212-215
Author(s):  
R. W. Jackson ◽  
A. Ortiz-Barredo ◽  
G. Tsiamis ◽  
D. Butcher ◽  
B. Wharton ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Pseudomonas Syringae Pathovars

scholarly journals Efficiency of Phage φ6 for Biocontrol of Pseudomonas syringae pv. syringae: An in Vitro Preliminary Study

2019 ◽  
Vol 7 (9) ◽  
pp. 286 ◽  
Author(s):  
Larindja A. M. Pinheiro ◽  
Carla Pereira ◽  
Carolina Frazão ◽  
Victor M. Balcão ◽  
Adelaide Almeida
Keyword(s):  
Solar Radiation ◽  
Host Range ◽  
Pseudomonas Syringae ◽  
Bacterial Resistance ◽  
Phage Therapy ◽  
Bacterial Species ◽  
Fruit Trees ◽  
Range Analysis ◽  
Maximum Reduction

Pseudomonas syringae is a plant-associated bacterial species that has been divided into more than 60 pathovars, with the Pseudomonas syringae pv. syringae being the main causative agent of diseases in a wide variety of fruit trees. The most common treatments for biocontrol of P. syringae pv. syringae infections has involved copper derivatives and/or antibiotics. However, these treatments should be avoided due to their high toxicity to the environment and promotion of bacterial resistance. Therefore, it is essential to search for new approaches for controlling P. syringae pv. syringae. Phage therapy can be a useful alternative tool to the conventional treatments to control P. syringae pv. syringae infections in plants. In the present study, the efficacy of bacteriophage (or phage) φ6 (a commercially available phage) was evaluated in the control of P. syringae pv. syringae. As the plants are exposed to the natural variability of physical and chemical parameters, the influence of pH, temperature, solar radiation and UV-B irradiation on phage φ6 viability was also evaluated in order to develop an effective phage therapy protocol. The host range analysis revealed that the phage, besides its host (P. syringae pv. syringae), also infects the Pseudomonas syringae pv. actinidiae CRA-FRU 12.54 and P. syringae pv. actinidiae CRA-FRU 14.10 strains, not infecting strains from the other tested species. Both multiplicities of infection (MOIs) tested, 1 and 100, were effective to inactivate the bacterium, but the MOI 1 (maximum reduction of 3.9 log CFU/mL) was more effective than MOI 100 (maximum reduction of 2.6 log CFU/mL). The viability of phage φ6 was mostly affected by exposure to UV-B irradiation (decrease of 7.3 log PFU/mL after 8 h), exposure to solar radiation (maximum reduction of 2.1 PFU/mL after 6 h), and high temperatures (decrease of 8.5 PFU/mL after 6 days at 37 °C, but a decrease of only 2.0 log PFU/mL after 67 days at 15 °C and 25 °C). The host range, high bacterial control and low rates of development of phage-resistant bacterial clones (1.20 × 10−3) suggest that this phage can be used to control P. syringae pv. syringae infections in plants, but also to control infections by P. syringae pv. actinidiae, the causal agent of bacterial canker of kiwifruit. Although the stability of phage φ6 was affected by UV-B and solar radiation, this can be overcome by the application of phage suspensions at the end of the day or at night.

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