A Rapid and Sensitive Method for Direct Detection of Erwinia amylovora in Symptomatic and Asymptomatic Plant Tissues by Polymerase Chain Reaction

2006 ◽  
Vol 154 (7-8) ◽  
pp. 469-473 ◽  
Author(s):  
A. Stoger ◽  
J. Schaffer ◽  
W. Ruppitsch
Keyword(s):  
Polymerase Chain Reaction ◽  
Erwinia Amylovora ◽  
Direct Detection ◽  
Plant Tissues ◽  
Chain Reaction ◽  
Asymptomatic Plant ◽  
Polymerase Chain ◽  
Sensitive Method

Polymerase Chain Reaction for the Direct Detection of Brucella spp. in Milk and Cheese

2001 ◽  
Vol 64 (2) ◽  
pp. 164-167 ◽  
Author(s):  
G. TANTILLO ◽  
A. DI PINTO ◽  
A. VERGARA ◽  
C. BUONAVOGLIA
Keyword(s):  
Polymerase Chain Reaction ◽  
Direct Detection ◽  
Polymerase Chain Reaction Test ◽  
Chain Reaction ◽  
Sheep Milk ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Quality Of Milk ◽  
Chain Reaction Test

A polymerase chain reaction test was developed to detect Brucella spp. directly in milk and cheese and optimized using primers for the BSCP-31 gene. A total of 46 cheese samples produced with sheep and goats milk were assayed, and Brucella spp. was detected in 46% of them, especially in cheese made from sheep milk. This method is of remarkable epidemiologic interest because it is an indirect test indicating the sanitary quality of milk used in dairy industries. The method showed good sensitivity and specificity. It is faster and less expensive than the conventional bacteriological assays.

Evaluation of Polymerase Chain Reaction for Direct Detection of Escherichia coli Strains in Environmental Samples

2007 ◽  
Vol 2 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Safa A. Sherfi . ◽  
Hamid A. Dirar . ◽  
Badr E. Hago . ◽  
Mohamed E. Ahmed . ◽  
Hassan A. Musa . ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Environmental Samples ◽  
Direct Detection ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals New sensitive method for the detection of the A3243G mutation of human mitochondrial deoxyribonucleic acid in diabetes mellitus patients by ligation-mediated polymerase chain reaction

1998 ◽  
Vol 44 (10) ◽  
pp. 2088-2093 ◽  
Author(s):  
Michiyo Urata ◽  
Machiko Wakiyama ◽  
Masanori Iwase ◽  
Makoto Yoneda ◽  
Sachiko Kinoshita ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Polymerase Chain Reaction ◽  
Conventional Method ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Nucleotide Position ◽  
Peripheral Blood Cells ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Sensitive Method

Abstract An adenine-to-guanine mutation at nucleotide position (np) 3243 in the mitochondrial tRNALeu(UUR) gene is closely associated with various clinical phenotypes of diabetes mellitus. Because the mutation creates a new restriction site for the restriction enzyme ApaI, the mutation is usually detected and quantified by ApaI cleavage of the PCR products including np 3243. The sensitivity of the conventional method is, however, 5–10% heteroplasmy. The percentage of heteroplasmy of the mutation is usually highest in the affected tissues and is much lower in peripheral blood cells, which are used most frequently for the analysis. The sensitivity of the conventional method, however, is not sufficient to detect the mutation from peripheral blood cells. Utilizing ligation-mediated polymerase chain reaction, we have developed a feasible and sensitive method to detect 0.01% heteroplasmy of the 3243 mutation in peripheral leukocytes.

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