Comparative Proteomic Analysis of Wheat Response to Powdery Mildew Infection in Wheat Pm30 Near-Isogenic Lines

2012 ◽  
Vol 160 (5) ◽  
pp. 229-236 ◽  
Author(s):  
Bo Wang ◽  
Chaojie Xie ◽  
Mingming Xin ◽  
Na Song ◽  
Zhongfu Ni ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Proteomic Analysis ◽  
Near Isogenic Lines ◽  
Comparative Proteomic Analysis ◽  
Isogenic Lines ◽  
Powdery Mildew Infection

scholarly journals Linked, if Not the Same, Mi-1 Homologues Confer Resistance to Tomato Powdery Mildew and Root-Knot Nematodes

2011 ◽  
Vol 24 (4) ◽  
pp. 441-450 ◽  
Author(s):  
Alireza Seifi ◽  
Isgouhi Kaloshian ◽  
Jack Vossen ◽  
Daidi Che ◽  
Kishor K. Bhattarai ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Powdery Mildew ◽  
Gene Cluster ◽  
Chromosome 6 ◽  
R Genes ◽  
Near Isogenic Lines ◽  
Root Knot Nematodes ◽  
Isogenic Lines ◽  
Tomato Chromosome ◽  
Tomato Powdery Mildew

On the short arm of tomato chromosome 6, a cluster of disease resistance (R) genes have evolved harboring the Mi-1 and Cf genes. The Mi-1 gene confers resistance to root-knot nematodes, aphids, and whiteflies. Previously, we mapped two genes, Ol-4 and Ol-6, for resistance to tomato powdery mildew in this cluster. The aim of this study was to investigate whether Ol-4 and Ol-6 are homologues of the R genes located in this cluster. We show that near-isogenic lines (NIL) harboring Ol-4 (NIL-Ol-4) and Ol-6 (NIL-Ol-6) are also resistant to nematodes and aphids. Genetically, the resistance to nematodes cosegregates with Ol-4 and Ol-6, which are further fine-mapped to the Mi-1 cluster. We provide evidence that the composition of Mi-1 homologues in NIL-Ol-4 and NIL-Ol-6 is different from other nematode-resistant tomato lines, Motelle and VFNT, harboring the Mi-1 gene. Furthermore, we demonstrate that the resistance to both nematodes and tomato powdery mildew in these two NIL is governed by linked (if not the same) Mi-1 homologues in the Mi-1 gene cluster. Finally, we discuss how Solanum crops exploit Mi-1 homologues to defend themselves against distinct pathogens.

Development of wheat near-isogenic lines for powdery mildew resistance

2005 ◽  
Vol 110 (4) ◽  
pp. 640-648 ◽  
Author(s):  
Ronghua Zhou ◽  
Zhendong Zhu ◽  
Xiuying Kong ◽  
Naxin Huo ◽  
Qingzhen Tian ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Near Isogenic Lines ◽  
Mildew Resistance ◽  
Isogenic Lines

scholarly journals Powdery Mildew-Induced Hormonal and Photosynthetic Changes in Barley Near Isogenic Lines Carrying Various Resistant Genes

2020 ◽  
Vol 21 (12) ◽  
pp. 4536 ◽  
Author(s):  
Diana Saja ◽  
Anna Janeczko ◽  
Balázs Barna ◽  
Andrzej Skoczowski ◽  
Michał Dziurka ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
Post Inoculation ◽  
Near Isogenic Lines ◽  
Leaf Reflectance ◽  
Isogenic Lines ◽  
Fluorescence Measurements ◽  
Resistant Genes ◽  
Psii Activity

The present work focused on the characterization of some physiological mechanisms activated upon powdery mildew inoculation of the susceptible barley cultivar Ingrid and its near-isogenic lines (NILs) carrying various resistant genes (Mla, Mlg and mlo). After inoculation with Blumeria graminis f. sp. hordei (Bgh), measurements of leaf reflectance and chlorophyll a fluorescence were performed 3 and 7 day post-inoculation (dpi), while hormone assays were made 7 dpi. Bgh-inoculated resistant genotypes were characterized by lowered leaf reflectance parameters that correlated with carotenoids (CRI) and water content (WBI) in comparison to inoculated Ingrid. The PSII activity (i.e., Fv/Fm, ETo/CSm and P.I.ABS) strongly decreased in susceptible Ingrid leaves when the disease symptoms became visible 7 dpi. In Mla plants with visible hypersensitive spots the PSII activity decreased to a lesser extent. Inoculation resulted in a very slight decrease of photosynthesis at later stage of infection in Mlg plants, whereas in resistant mlo plants the PSII activity did not change. Chlorophyll a fluorescence measurements allowed presymptomatic detection of infection in Ingrid and Mla. Changes in the homeostasis of 22 phytohormones (cytokinins, auxins, gibberellins and the stress hormones JA, SA and ABA) in powdery mildew inoculated barley are discussed in relation to resistance against this biotrophic pathogen.

Proteomic analysis of blast-resistant near-isogenic lines derived from japonica rice, var. Yunyin, infected with Magnaporthe oryzae

2014 ◽  
Vol 59 (32) ◽  
pp. 4312-4322 ◽  
Author(s):  
Weimin Xue ◽  
Xiaohui Mao ◽  
Yidong Wei ◽  
Lian Ling ◽  
Yongsheng Zhu ◽  
...  
Keyword(s):  
Magnaporthe Oryzae ◽  
Proteomic Analysis ◽  
Japonica Rice ◽  
Near Isogenic Lines ◽  
Isogenic Lines

Operation of resistance genes in wheat to Erysiphe graminis f.sp. tritici against E. graminis f.sp. agropyri

Genome ◽  
1990 ◽  
Vol 33 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Y. Tosa ◽  
S. Tada
Keyword(s):  
Powdery Mildew ◽  
Common Wheat ◽  
Resistance Genes ◽  
Wheat Cultivar ◽  
Erysiphe Graminis ◽  
Near Isogenic Lines ◽  
Avirulence Genes ◽  
Common Wheat Cultivar ◽  
Isogenic Lines ◽  
The Common

The common wheat cultivar 'Chancellor' and its near-isogenic lines carrying resistance genes to Erysiphe graminis f.sp. tritici were inoculated with 240 F1 cultures derived from a cross between E. graminis f.sp. agropyri, Ak-1, and E. graminis f.sp. tritici, Tk-1. Segregation patterns of avirulent and virulent cultures suggested that the F1 population carries avirulence genes corresponding to Pm1, Pm2, Pm3a, and Pm3b. These avirulence genes were considered to be derived from Ak-1. It was therefore concluded that Pm1, Pm2, Pm3a, and Pm3b, resistance genes to E. graminis f.sp. tritici, operate against E. graminis f.sp. agropyri.Key words: powdery mildew, Erysiphe graminis, resistance, wheat, wheatgrass.

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