Immunohistochemical Detection of Porcine Teschovirus Antigen in the Formalin-fixed Paraffin-embedded Specimens from Pigs Experimentally Infected with Porcine Teschovirus

Brucella melitensis, a worldwide zoonotic pathogen, is a significant cause of abortion in sheep and goats in some countries. The present study was carried out to determine, by immunohistochemistry, the presence of B. melitensis antigens in 110 naturally occurring aborted sheep fetuses. Sections of lung, liver, kidney, and spleen of each fetus were stained with immunoperoxidase to detect Brucella antigens. Brucella melitensis antigens were detected in 33 of 110 fetuses (30%). In the 33 positive cases, Brucella antigens were found in lung (25 [22.7%]), liver (21 [19%]), spleen (13 [11.8%]), and kidney (6 [5.4%]). Microscopic studies demonstrated that Brucella antigens were mainly located in the cytoplasm of macrophages and neutrophils of the lung, and in the cytoplasm of macrophages in the portal infiltrates and Kupffer cells of the liver. It was concluded that immunohistochemistry in formalin-fixed, paraffin-embedded tissues is a useful tool for the diagnosis of spontaneous ovine abortion caused by B. melitensis.

Download Full-text

Specificity and sensitivity of immunohistochemical detection of factor VIII/von Willebrand factor antigen in formalin-fixed paraffin-embedded tissue.

Journal of Histochemistry & Cytochemistry ◽

10.1177/30.4.6801111 ◽

1982 ◽

Vol 30 (4) ◽

pp. 371-377 ◽

Cited By ~ 64

Author(s):

R D McComb ◽

T R Jones ◽

S V Pizzo ◽

D D Bigner

Keyword(s):

Endothelial Cells ◽

Von Willebrand Factor ◽

Immunohistochemical Detection ◽

Normal Tissues ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Von Willebrand ◽

Willebrand Factor ◽

Factor Antigen ◽

Formalin Fixed

The immunohistochemical detection of factor VIII/von Willebrand factor antigen (FVIII/vWF-AG) in formalin-fixed paraffin-embedded tissues was investigated using the peroxidase-antiperoxidase (PAP) method. Highly purified human FVIII/vWF was used to raise rabbit anti-FVIII/vWF-AG serum. In addition to anti-FVIII/vWF-AG activity, the unabsorbed antiserum had anti-IgG, anti-IgM, and anti-alpha2-macroglobulin specificities. Following exhaustive absorption with these proteins, the antiserum reacted monospecifically for FVIII/vWF-AG in immunodiffusion, immunoelectrophoresis, and PAP immunohistochemistry. Sections of normal tissues from six patients and a total of 43 neoplasms were examined. Treatment of the tissue sections with trypsin prior to application of the antiserum markedly increased the sensitivity of FVIII/vWF-AG detection. The positive staining for FVIII/vWF-AG was restricted to endothelial cells in both neoplastic and nonneoplastic tissue. In general, the hyperplastic endothelia in neoplastic and reactive tissues stained more intensely than those in normal tissues. Expression of FVIII/vWF-AG by nonendothelial neoplastic cells was not observed. FVIII/vWF-AG is a reliable marker for endothelial cells.

Download Full-text

Immunohistochemical Detection of Francisella tularensis in Formalin-Fixed Paraffin-Embedded Tissue

Applied Immunohistochemistry & Molecular Morphology ◽

10.1097/00129039-199906000-00004 ◽

1999 ◽

Vol 7 (2) ◽

pp. 122-126

Author(s):

Jeannette Guarner ◽

Patricia W. Greer ◽

Jeanine Bartlett ◽

May C. Chu ◽

Wun-Ju Shieh ◽

...

Keyword(s):

Francisella Tularensis ◽

Immunohistochemical Detection ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

Download Full-text

Immunohistochemical Detection of Canine Adenovirus in Paraffin Sections of Liver

Veterinary Pathology ◽

10.1177/030098588602300419 ◽

1986 ◽

Vol 23 (4) ◽

pp. 478-484 ◽

Cited By ~ 26

Author(s):

P. M. Rakich ◽

K. W. Prasse ◽

P. D. Lukert ◽

L. M. Cornelius

Keyword(s):

Immunohistochemical Detection ◽

Hepatic Inflammation ◽

Paraffin Sections ◽

Inflammatory Lesions ◽

Long Term Stability ◽

Formalin Fixed Paraffin ◽

Wide Range ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

An avidin-biotin immunoperoxidase procedure was optimized for detection of canine adenoviral antigens in formalin-fixed, paraffin-embedded liver. Long-term stability of viral antigen was shown by successful demonstration of virus in liver tissue preserved up to six years from dogs with infectious canine hepatitis. This immunohistochemical stain was applied to sections from livers with a wide range of inflammatory lesions. Examination of sections from 53 dogs yielded five livers with small amounts of adenovirus. An additional virus-positive liver was identified from a dog with no hepatic inflammation. Although a cause and effect relationship remains to be determined, these findings suggest a possible connection between canine adenovirus and spontaneous chronic hepatitis.

Download Full-text