Immunohistochemical Detection of Canine Adenovirus in Paraffin Sections of Liver

An avidin-biotin immunoperoxidase procedure was optimized for detection of canine adenoviral antigens in formalin-fixed, paraffin-embedded liver. Long-term stability of viral antigen was shown by successful demonstration of virus in liver tissue preserved up to six years from dogs with infectious canine hepatitis. This immunohistochemical stain was applied to sections from livers with a wide range of inflammatory lesions. Examination of sections from 53 dogs yielded five livers with small amounts of adenovirus. An additional virus-positive liver was identified from a dog with no hepatic inflammation. Although a cause and effect relationship remains to be determined, these findings suggest a possible connection between canine adenovirus and spontaneous chronic hepatitis.

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Differential long-term stability of microRNAs and RNU6B snRNA in 12–20 year old archived formalin-fixed paraffin-embedded specimens

BMC Cancer ◽

10.1186/s12885-016-3008-4 ◽

2017 ◽

Vol 17 (1) ◽

Cited By ~ 18

Author(s):

Sarah B. Peskoe ◽

John R. Barber ◽

Qizhi Zheng ◽

Alan K. Meeker ◽

Angelo M. De Marzo ◽

...

Keyword(s):

Term Stability ◽

Long Term Stability ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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Immunohistochemical Detection of Brucella Melitensis Antigens in Cases of Naturally Occurring Abortions in Sheep

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063870802000616 ◽

2008 ◽

Vol 20 (6) ◽

pp. 803-806 ◽

Cited By ~ 16

Author(s):

Fatma Ilhan ◽

Zabit Yener

Keyword(s):

Kupffer Cells ◽

Brucella Melitensis ◽

Immunohistochemical Detection ◽

Zoonotic Pathogen ◽

Sheep And Goats ◽

Naturally Occurring ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Microscopic Studies ◽

Formalin Fixed

Brucella melitensis, a worldwide zoonotic pathogen, is a significant cause of abortion in sheep and goats in some countries. The present study was carried out to determine, by immunohistochemistry, the presence of B. melitensis antigens in 110 naturally occurring aborted sheep fetuses. Sections of lung, liver, kidney, and spleen of each fetus were stained with immunoperoxidase to detect Brucella antigens. Brucella melitensis antigens were detected in 33 of 110 fetuses (30%). In the 33 positive cases, Brucella antigens were found in lung (25 [22.7%]), liver (21 [19%]), spleen (13 [11.8%]), and kidney (6 [5.4%]). Microscopic studies demonstrated that Brucella antigens were mainly located in the cytoplasm of macrophages and neutrophils of the lung, and in the cytoplasm of macrophages in the portal infiltrates and Kupffer cells of the liver. It was concluded that immunohistochemistry in formalin-fixed, paraffin-embedded tissues is a useful tool for the diagnosis of spontaneous ovine abortion caused by B. melitensis.

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Specificity and sensitivity of immunohistochemical detection of factor VIII/von Willebrand factor antigen in formalin-fixed paraffin-embedded tissue.

Journal of Histochemistry & Cytochemistry ◽

10.1177/30.4.6801111 ◽

1982 ◽

Vol 30 (4) ◽

pp. 371-377 ◽

Cited By ~ 64

Author(s):

R D McComb ◽

T R Jones ◽

S V Pizzo ◽

D D Bigner

Keyword(s):

Endothelial Cells ◽

Von Willebrand Factor ◽

Immunohistochemical Detection ◽

Normal Tissues ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Von Willebrand ◽

Willebrand Factor ◽

Factor Antigen ◽

Formalin Fixed

The immunohistochemical detection of factor VIII/von Willebrand factor antigen (FVIII/vWF-AG) in formalin-fixed paraffin-embedded tissues was investigated using the peroxidase-antiperoxidase (PAP) method. Highly purified human FVIII/vWF was used to raise rabbit anti-FVIII/vWF-AG serum. In addition to anti-FVIII/vWF-AG activity, the unabsorbed antiserum had anti-IgG, anti-IgM, and anti-alpha2-macroglobulin specificities. Following exhaustive absorption with these proteins, the antiserum reacted monospecifically for FVIII/vWF-AG in immunodiffusion, immunoelectrophoresis, and PAP immunohistochemistry. Sections of normal tissues from six patients and a total of 43 neoplasms were examined. Treatment of the tissue sections with trypsin prior to application of the antiserum markedly increased the sensitivity of FVIII/vWF-AG detection. The positive staining for FVIII/vWF-AG was restricted to endothelial cells in both neoplastic and nonneoplastic tissue. In general, the hyperplastic endothelia in neoplastic and reactive tissues stained more intensely than those in normal tissues. Expression of FVIII/vWF-AG by nonendothelial neoplastic cells was not observed. FVIII/vWF-AG is a reliable marker for endothelial cells.

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Improved RT-PCR Amplification for Molecular Analyses with Long-term Preserved Formalin-fixed, Paraffin-embedded Tissue Specimens

Journal of Histochemistry & Cytochemistry ◽

10.1369/jhc.5a6859.2006 ◽

2006 ◽

Vol 54 (7) ◽

pp. 773-780 ◽

Cited By ~ 28

Author(s):

Kiyohiro Hamatani ◽

Hidetaka Eguchi ◽

Keiko Takahashi ◽

Kazuaki Koyama ◽

Mayumi Mukai ◽

...

Keyword(s):

Pcr Amplification ◽

Rt Pcr ◽

Molecular Analyses ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Tissue Specimens ◽

Formalin Fixed

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Immunohistochemical Detection of Francisella tularensis in Formalin-Fixed Paraffin-Embedded Tissue

Applied Immunohistochemistry & Molecular Morphology ◽

10.1097/00129039-199906000-00004 ◽

1999 ◽

Vol 7 (2) ◽

pp. 122-126

Author(s):

Jeannette Guarner ◽

Patricia W. Greer ◽

Jeanine Bartlett ◽

May C. Chu ◽

Wun-Ju Shieh ◽

...

Keyword(s):

Francisella Tularensis ◽

Immunohistochemical Detection ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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Immunohistochemical Detection of Porcine Teschovirus Antigen in the Formalin-fixed Paraffin-embedded Specimens from Pigs Experimentally Infected with Porcine Teschovirus

Journal of Veterinary Medicine Series A ◽

10.1111/j.1439-0442.2007.00974.x ◽

2007 ◽

Vol 54 (10) ◽

pp. 571-574 ◽

Cited By ~ 9

Author(s):

M. Yamada ◽

Y. Kaku ◽

K. Nakamura ◽

M. Yoshii ◽

Y. Yamamoto ◽

...

Keyword(s):

Immunohistochemical Detection ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Porcine Teschovirus ◽

Formalin Fixed

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NeuN: a useful neuronal marker for diagnostic histopathology.

Journal of Histochemistry & Cytochemistry ◽

10.1177/44.10.8813082 ◽

1996 ◽

Vol 44 (10) ◽

pp. 1167-1171 ◽

Cited By ~ 338

Author(s):

H K Wolf ◽

R Buslei ◽

R Schmidt-Kastner ◽

P K Schmidt-Kastner ◽

T Pietsch ◽

...

Keyword(s):

Neuronal Cell ◽

Cell Types ◽

Specific Protein ◽

Antigen Retrieval ◽

Neuronal Marker ◽

Formalin Fixed Paraffin ◽

Wide Range ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed ◽

Diagnostic Histopathology

The monoclonal antibody A60 specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most neuronal cell types of vertebrates. In this study we demonstrate the potential use of NeuN as a diagnostic neuronal marker using a wide range of formalin-fixed, paraffin-embedded human surgical and autopsy specimens from the central and peripheral nervous system. After microwave antigen retrieval, almost all neuronal populations revealed strong immunoreactivity for NeuN in nuclei, perikarya, and some proximal neuronal processes, whereas more distal axon cylinders and dendritic ramifications were not stained. The stain greatly enhanced the gray matter architecture. NeuN immunoreactivity was not detected in Purkinje cells, most neurons of the internal nuclear layer of the retina, and in sympathetic chain ganglia. We examined nine gangliogliomas and 14 dysembryoplastic neuroepithelial tumors, one ganglioneuroma, and one dysplastic cerebellar gangliocytoma. The neuronal component of all of these lesions showed marked immunoreactivity for NeuN. In addition, NeuN immunoreactivity was focally seen in one of seven medulloblastomas with prominent neuronal differentiation. There was no staining of non-neuronal structures. The results indicate that NeuN immunoreactivity is a sensitive and specific neuronal marker in formalin-fixed, paraffin-embedded tissues, and may be useful in diagnostic histopathology.

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