S-RNasegenotyping and incompatibility group assignment by PCR and pollination experiments in Japanese plum

Diploid japanese plum (Prunus salicina Lindl.) cultivars are commonly self-incompatible. To date, 14 incompatibility alleles (S-alleles) have been identified and labeled with alphabetical (S a-S n) and 5 with numeric codes (S 1, S 3-S 6). We applied polymerase chain reaction amplification of the S-RNase alleles with degenerate and allele-specific primers in 10 japanese plum cultivars and two pluots of unknown incompatibility alleles. Besides DNA sequencing, an additional method for the exact length determination of the first intron region was used for the first time for S-genotype japanese plums. The S 3-allele was shown to correspond to S k in the alphabetic nomenclature, S 4 to S c, S 5 to S e, and S 6 to S f. The S 5-allele-specific primer can be used as a reliable marker for self-compatibility in japanese plum. ‘Black Amber’, ‘October Sun’, ‘TC Sun’, and ‘Super Giant’ share the S b S c genotype, which was confirmed by test crosses. These cultivars belong to the widest incompatibility group currently known in japanese plum. An additional incompatibility group (S c S h) was established, including ‘Green Sun’ and ‘Queen Rosa’, a cultivar formerly known as a universal donor. By incorporating all previous and recent results, a table was assembled including 49 cultivars assigned to I–VII incompatibility groups, to the self-compatible group and to the group O of unique genotypes. These data may considerably contribute to further growing and breeding activities.

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A Variant Group Assignment Three Dimensional Model Using Pattern Recognition Technique

Journal of Computer and Mathematical Sciences ◽

10.29055/jcms/1126 ◽

2019 ◽

Vol 10 (6) ◽

pp. 1382-1394

Author(s):

R. Vijayalakshmi ◽

V. K. Soma Sekhar Srinivas ◽

E. Manjoolatha ◽

G. Rajeswari ◽

M. Sundaramurthy

Keyword(s):

Pattern Recognition ◽

Three Dimensional ◽

Dimensional Model ◽

Three Dimensional Model ◽

Pattern Recognition Technique ◽

Group Assignment ◽

Recognition Technique

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Performance analysis of multinomial distribution of group assignment framed ALOHA for multiple RFID

2014 11th International Conference on Electrical Engineering/Electronics, Computer, Telecommunications and Information Technology (ECTI-CON) ◽

10.1109/ecticon.2014.6839857 ◽

2014 ◽

Cited By ~ 1

Author(s):

Bancha Lertmaneekarn ◽

Natthapol Moolsiri ◽

Thanyawarat Pawasopon ◽

Suvepon Sittichivapak

Keyword(s):

Performance Analysis ◽

Multinomial Distribution ◽

Group Assignment

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Hypotension in Preterm Infants (HIP) randomised trial

Archives of Disease in Childhood - Fetal and Neonatal Edition ◽

10.1136/archdischild-2020-320241 ◽

2021 ◽

pp. fetalneonatal-2020-320241

Author(s):

Eugene M Dempsey ◽

Keith J Barrington ◽

Neil Marlow ◽

Colm Patrick Finbarr O'Donnell ◽

Jan Miletin ◽

...

Keyword(s):

Brain Injury ◽

Primary Outcome ◽

Randomised Trial ◽

Intraventricular Haemorrhage ◽

Standard Group ◽

Double Blind ◽

Group Assignment ◽

Standard Management ◽

Postmenstrual Age ◽

Registration Number

ObjectiveTo determine whether restricting the use of inotrope after diagnosis of low blood pressure (BP) in the first 72 hours of life affects survival without significant brain injury at 36 weeks of postmenstrual age (PMA) in infants born before 28 weeks of gestation.DesignDouble-blind, placebo-controlled randomised trial. Caregivers were masked to group assignment.Setting10 sites across Europe and Canada.ParticipantsInfants born before 28 weeks of gestation were eligible if they had an invasive mean BP less than their gestational age that persisted for ≥15 min in the first 72 hours of life and a cerebral ultrasound free of significant (≥ grade 3) intraventricular haemorrhage.InterventionParticipants were randomly assigned to saline bolus followed by either a dopamine infusion (standard management) or placebo (5% dextrose) infusion (restrictive management).Primary outcomeSurvival to 36 weeks of PMA without severe brain injury.ResultsThe trial terminated early due to significant enrolment issues (7.7% of planned recruitment). 58 infants were enrolled between February 2015 and September 2017. The two groups were well matched for baseline variables. In the standard group, 18/29 (62%) achieved the primary outcome compared with 20/29 (69%) in the restrictive group (p=0.58). Additional treatments for low BP were used less frequently in the standard arm (11/29 (38%) vs 19/29 (66%), p=0.038).ConclusionThough this study lacked power, we did not detect major differences in clinical outcomes between standard or restrictive approach to treatment. These results will inform future studies in this area.Trial registration numberNCT01482559, EudraCT 2010-023988-17.

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MBRS-47. RAPID MOLECULAR SUBGROUPING OF MEDULLOBLASTOMA BASED ON DNA METHYLATION BY NANOPORE SEQUENCING

Neuro-Oncology ◽

10.1093/neuonc/noaa222.556 ◽

2020 ◽

Vol 22 (Supplement_3) ◽

pp. iii406-iii406

Author(s):

Julien Masliah-Planchon ◽

Elodie Girard ◽

Philipp Euskirchen ◽

Christine Bourneix ◽

Delphine Lequin ◽

...

Keyword(s):

Dna Methylation ◽

Single Molecule ◽

Nanopore Sequencing ◽

Molecular Subgroup ◽

Group Assignment ◽

Group 4 ◽

Methylation Assay ◽

Tumor Group ◽

Long Read ◽

Group 3

Abstract Medulloblastoma (MB) can be classified into four molecular subgroups (WNT group, SHH group, group 3, and group 4). The gold standard of assignment of molecular subgroup through DNA methylation profiling uses Illumina EPIC array. However, this tool has some limitation in terms of cost and timing, in order to get the results soon enough for clinical use. We present an alternative DNA methylation assay based on nanopore sequencing efficient for rapid, cheaper, and reliable subgrouping of clinical MB samples. Low-depth whole genome with long-read single-molecule nanopore sequencing was used to simultaneously assess copy number profile and MB subgrouping based on DNA methylation. The DNA methylation data generated by Nanopore sequencing were compared to a publicly available reference cohort comprising over 2,800 brain tumors including the four subgroups of MB (Capper et al. Nature; 2018) to generate a score that estimates a confidence with a tumor group assignment. Among the 24 MB analyzed with nanopore sequencing (six WNT, nine SHH, five group 3, and four group 4), all of them were classified in the appropriate subgroup established by expression-based Nanostring subgrouping. In addition to the subgrouping, we also examine the genomic profile. Furthermore, all previously identified clinically relevant genomic rearrangements (mostly MYC and MYCN amplifications) were also detected with our assay. In conclusion, we are confirming the full reliability of nanopore sequencing as a novel rapid and cheap assay for methylation-based MB subgrouping. We now plan to implement this technology to other embryonal tumors of the central nervous system.

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Practice of Telehealth in Otolaryngology: A Scoping Review in the Era of COVID-19

Otolaryngology ◽

10.1177/01945998211013751 ◽

2021 ◽

pp. 019459982110137

Author(s):

Joseph N. Gonzalez ◽

Lucas G. Axiotakis ◽

Victoria X. Yu ◽

David A. Gudis ◽

Jonathan B. Overdevest

Keyword(s):

Outcome Measures ◽

Scoping Review ◽

Medical Center ◽

Randomized Study ◽

Marked Change ◽

Policy Decision ◽

Columbia University ◽

Group Assignment ◽

Scoping Reviews ◽

Full Text Review

Objective The COVID-19 pandemic has spurred widespread adoption and advancement in telehealth activities, representing a marked change in otolaryngology practice patterns. The present study undertakes a scoping review of research focused on telehealth in otolaryngology (teleotolaryngology) to identify key themes and commonly utilized outcome measures that will assist future development in this growing field. Data Sources PubMed, Embase, and Cochrane databases and reference review. Review Methods Per guidelines of the PRISMA Extension for Scoping Reviews, we performed database queries using a comprehensive search strategy developed in collaboration with research librarians at the Columbia University Irving Medical Center. We identified 596 unique references to undergo title and abstract review by 2 independent reviewers, leaving 439 studies for full-text review. Results We included 285 studies for extraction of notable findings, leaving 262 unique studies after accounting for content overlap. We identified core outcome measures, including patient and provider satisfaction, costs and benefits, quality of care, feasibility, and access to care. Publication volume increased markedly over time, though only 4% of studies incorporated randomized study group assignment. Using an iterative approach to thematic development, we organized article content across 5 main themes: (1) exploration of teleotolaryngology evolution, (2) role in virtual clinical encounters, (3) applications in interdisciplinary care and educational initiatives, (4) emerging and innovative technologies, and (5) barriers to implementation. Conclusion This scoping review of teleotolaryngology documents its evolution and identifies current use cases, limitations, and emerging applications, providing a foundation from which to build future studies, inform policy decision making, and facilitate implementation where appropriate.

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