Sex Identification of the Black Swan (Cygnus atratus) using the Locus-specific PCR and Implications for its Reproduction

2005 ◽  
Vol 40 (3) ◽  
pp. 196-198 ◽  
Author(s):  
P-J He ◽  
J-Q Yu ◽  
S-G Fang
Keyword(s):  
Sex Identification ◽  
Black Swan ◽  
Specific Pcr

scholarly journals Lethal infection caused by Tetratrichomonas gallinarum in black swans (Cygnus atratus)

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Shengyong Feng ◽  
Han Chang ◽  
Yutian Wang ◽  
Fubing Luo ◽  
Qiaoxing Wu ◽  
...  
Keyword(s):  
Host Range ◽  
Microscopic Examination ◽  
Fibrous Tissue ◽  
Black Swan ◽  
Mononuclear Cell Infiltration ◽  
Case Presentation ◽  
Specific Pcr ◽  
Black Swans ◽  
Lethal Infection ◽  
Mild Diarrhea

Abstract Background Tetratrichomonas gallinarum is parasitic protozoa with a wide host range. However, its lethal infection is rare reported. Case presentation Here, we described the first lethal cases of T. gallinarum infection in black swans in China. Five black swans died within a week in succession without obvious symptoms except mild diarrhea. At necropsy, severe lesions were observed in caeca with thickened caecal walls and hemorrhages in the mucosa. A large number of moving trophozoites were found in the contents of the cecum by microscopic examination. The livers were enlarged with multiple bleeding spots on the surface. Histopathology of the livers showed mononuclear cell infiltration and moderate hyperplasia of fibrous tissue. The histopathology of the cecum showed that the villi of the cecum were edematous. Finally, the presence of T. gallinarum was determined by specific PCR andin-situ hybridization assay. Additionally, common pathogens that can cause similar symptoms were excluded. Conclusions The death of the black swan was caused by T. gallinarum, suggesting that the parasite might be a new threat to the Cygnus birds.

scholarly journals Identification of sex in F1 progenies of hop (Humulus lupulus L.) by molecular marker

2011 ◽  
Vol 48 (No. 7) ◽  
pp. 318-321 ◽  
Author(s):  
J. Patzak ◽  
P. Vejl ◽  
S. Skupinová ◽  
V. Nesvadba
Keyword(s):  
Molecular Marker ◽  
Marker Assisted Selection ◽  
Sex Identification ◽  
Humulus Lupulus ◽  
Statistic Analysis ◽  
Dioecious Plant ◽  
Pcr Marker ◽  
Reliable Identification ◽  
Specific Pcr ◽  
Humulus Lupulus L

Sex identification for hop (Humulus lupulus L.), which is a&nbsp;dioecious plant, is very important for breeding process. The use of molecular methods for marker-assisted selection (MAS) provides rapid and reliable identification of sex in F<sub>1</sub> progenies. In the first, we proved the use of specific PCR molecular marker for evaluation of sex in selected plants. This marker was not amplified in 3 from 35 male plants. In the next, we successfully analysed 770 genotypes of F<sub>1</sub> progenies of three crossings. The amplified differences were found in 4 male plants of crossing Sm01 H28, 8 male plants of crossing Sm01 H29 and 5 female plants of crossing Sm00 H20. Statistic analysis confirmed that sex ratios of all F<sub>1</sub> progenies were significantly coincident. Obtained results confirmed that this specific PCR marker successfully identified sex of juvenile hop plants in F<sub>1</sub> progenies and effectively improved breeding process.

Sex Identification of Four Penguin Species Using Locus-Specific PCR

Zoo Biology ◽  
2012 ◽  
Vol 32 (3) ◽  
pp. 257-261 ◽  
Author(s):  
Peijun Zhang ◽  
Jiabo Han ◽  
Quansheng Liu ◽  
Junxin Zhang ◽  
Xianfeng Zhang
Keyword(s):  
Sex Identification ◽  
Specific Pcr ◽  
Penguin Species

Simple and Rapid Detection of Factor V Leiden by Allele-specific PCR Amplification

1996 ◽  
Vol 75 (05) ◽  
pp. 757-759 ◽  
Author(s):  
Rainer Blasczyk ◽  
Markus Ritter ◽  
Christian Thiede ◽  
Jenny Wehling ◽  
Günter Hintz ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Activated Protein C ◽  
Factor V Leiden ◽  
Pcr Amplification ◽  
Factor V ◽  
Specific Pcr ◽  
Pcr Rflp ◽  
Allele Specific ◽  
Specific Amplification ◽  
Allele Specific Pcr

SummaryResistance to activated protein C is the most common hereditary cause for thrombosis and significantly linked to factor V Leiden. In this study, primers were designed to identify the factor V mutation by allele-specific PCR amplification. 126 patients with thromboembolic events were analysed using this technique, PCR-RFLP and direct sequencing. The concordance between these techniques was 100%. In 27 patients a heterozygous factor VGln506 mutation was detected, whereas one patient with recurrent thromboembolism was homozygous for the point mutation. Due to its time- and cost-saving features allele-specific amplification should be considered for screening of factor VGln506.

The Black Swan: Yeats and the Science of the Unknown

2017 ◽  
Vol 47 (2) ◽  
pp. 350-359
Author(s):  
Aoife Lynch
Keyword(s):  
Twentieth Century ◽  
Scientific Theory ◽  
Black Swan ◽  
Max Planck ◽  
Universal History ◽  
New Concepts ◽  
Wave Particle Duality ◽  
Paradigmatic Change

This essay views science as a creative mask for the poetry and philosophy of W.B. Yeats. It explores the changing worldview which occurred at the beginning of the twentieth century with the discovery of wave-particle duality by Max Planck in 1900. It considers the new concepts of reality which arose at this time in relation to modernism and Yeats's response to the paradigmatic change of era he was a part of. Accordingly, the poet's understanding of universal history in A Vision (1925, 1937) is used alongside close readings of his poetry to evince an argument which unites that poetry with philosophy, scientific theory, and modernism as aspects of one universe of knowledge which refracts different aspects of itself through the prism of time.

Сочетанные генотипы генов, кодирующих белки холецистокининергической системы и ферменты фолатного цикла в значительной степени связаны с мигренью

2020 ◽  
Author(s):  
J.E. Azimova ◽  
E.A. Klimov ◽  
E.A. Naumova ◽  
Z.G. Kokaeva ◽  
A.I. Zaitseva ◽  
...  
Keyword(s):  
Population Control ◽  
Statistical Significance ◽  
Episodic Migraine ◽  
Protective Role ◽  
Control Group ◽  
Maoa Gene ◽  
Specific Pcr ◽  
Genotype Frequencies ◽  
Biochemical Systems ◽  
Combined Genotypes

Перспективным в изучении биомаркеров мигрени может быть многолокусный анализ, в частности, анализ частот сочетанных генотипов. Цель исследования - поиск составных генетических биомаркеров индивидуальной предрасположенности к мигрени, полученных на основе полиморфизмов генов, уже показавших статистическую значимость при однолокусном ассоциативном анализе. Методика. Обследовано 155 пациентов с мигренью (104 пациента с эпизодической мигренью, 51 - с хронической мигренью), наблюдавшихся в Университетской клинике головной боли (Москва). Все пациенты - представители белой расы, жители Московского региона. Возраст пациентов - 30-50 лет. Контроль составили 365 необследованных лиц (популяционный контроль). Выявление исследуемых 22 генов (всего 31 SNP) осуществляли методом ПЦР, ПЦР-ПДРФ, аллель-специфичной ПЦР и ПЦР в реальном времени. Выявление ассоциированных с мигренью сочетанных генотипов проводили с использованием программы анализа полигенных данных APSampler v3.6. Результаты. Выявлено 8 сочетанных генотипов с высокой статистически значимой ассоциацией с мигренью (ОШ>20,0). В состав сочетанных генотипов вошли гены: CCKAR, CCKBR, COMT, MTHFR, MTR, MTRR. Так же выявлено 4 защитных сочетанных генотипа (ОШ<0,02), основным в которых является ген MAOA. Заключение. Полученные данные об ассоциированных с мигренью сочетанных генотипах указывают на значимую роль в патогенезе заболевания 2 биохимических систем: 1) холецистокининергической системы, регулирующей выброс и обратный захват дофамина, и 2) фолатного цикла, в ходе работы которого гомоцистеин метаболизируется в метионин. Результаты, полученные в данном исследовании, позволяют говорить о защитной роли аллеля VNT:R4 гена MAOA.Multilocus analysis, specifically, analysis of combined genotype frequencies may be promising in studying migraine biomarkers. The aim of the study was to search for composite genetic biomarkers, which would predict individual predisposition to migraine, obtained on the basis of gene polymorphisms that have already shown a statistical significance in a single-locus associative analysis. Methods. 155 patients with migraine aging 41.7 ± 12.5 who had been followed up at the University Clinic of Headache, Moscow, were evaluated (104 patients with episodic migraine and 51 with chronic migraine). All patients were white and residents of the Moscow region. The control group included 365 unexamined individuals (population control). Identification of The 22 genes under study (total, 31 SNPs) were identified by PCR, PCR-RFLP, allele-specific PCR, and real-time PCR. Combined genotypes associated with migraine were identified using the APSampler v3.6 software for polygenic data analysis. Results. Eight combined genotypes were identified with a highly significant association with migraine (OR> 20.0). The combined genotypes included the CCKAR, CCKBR, COMT, MTHFR, MTR, and MTRR genes. Four protective combined genotypes were also identified (OS <0.02) with the MAOA gene as the major one. Conclusion. Our data on migraine-associated combined genotypes indicate a significant role in the migraine pathogenesis of two biochemical systems, i) the cholecystokininergic system that regulates the release and reuptake of dopamine, and ii) the folate cycle, where homocysteine is metabolized to methionine. The results obtained in this study suggest a protective role of the VNT: R4 allele of the MAOA gene.

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