PRACTICAL APPLICATIONS OF NEURONAL TISSUE CULTURE IN IN VITRO TOXICOLOGY

Abstract There are some limitations in the practical applications of in vitro date palm tissue culture, such as low multiplication efficiency, low rooting rate, and high mortality experienced by in vitro raised plantlets during laboratory to soil transfer. The objective of the present study is to determine the effect of polyamines (putrescine "PUT" and spermidine" SPD") and silver thiosulfate (STS) on enhancing propagation of date palm cv Quntar in vitro. Media supplemented with 75 mg L-1 SPD in combination with 10 mgL-1 STS gave the highest percentage of callus producing buds (83.34%) and average bud formation (16.3) per jar. The addition of PUT and STS to the medium was most effective in root regeneration and the number of roots per shoot, where the best result 91.67% and 6.37 roots per shoot, respectively, were obtained using 75 mgL-1 PUT and 10 mgL-1 STS, resulting in fast-growing plantlets during acclimatization phase, reaching 90% of plant survival. The genetic fidelity assessment of plants derived from micropropagation was confirmed by RAPD analysis. Four operon primers were used, and all of them showed amplified unambiguous (OPA02, OPC-04, OPD-07, and OPE-15). All generated bands were monomorphic and had no variation among the tissue culture-derived plants tested. Accordingly, these results indicate that adding polyamines and silver thiosulfate to the nutrient medium of date palm cv. Quntar is beneficial in improving shoot organogenesis, rooting, and production of genetically stable date palm plants.

Download Full-text

The Effect of Polyamines and Silver Thiosulphate on Micropropagation of Date Palm Followed by Genetic Stability Assessment

10.21203/rs.3.rs-1175104/v1 ◽

2022 ◽

Author(s):

Ahmed M.W Al-Mayahi

Keyword(s):

Tissue Culture ◽

Genetic Stability ◽

Date Palm ◽

Genetic Fidelity ◽

Silver Thiosulphate ◽

Silver Thiosulfate ◽

Practical Applications ◽

Fidelity Assessment ◽

Soil Transfer

Abstract There are some limitations in the practical applications of in vitro date palm tissue culture, such as low multiplication efficiency, low rooting rate, and high mortality experienced by in vitro raised plantlets during laboratory to soil transfer. This study’s objective was to investigate the effect of the two types of polyamines (putrescine "PUT" and spermidine" SPD") in combination with silver thiosulfate (STS) on the growth and development and genetic stability of cultures of Quntar cultivar. Media supplemented with 75 mg L−1 SPD in combination with 10 mgL−1 STS gave the highest percentage of callus producing buds (83.34%) and average bud formation (16.3) per jar. The addition of PUT and STS to the medium was most effective in root formation and the number of roots per shoot, where the best result 91.67% and 6.37 roots per shoot, respectively, were obtained using 75 mgL−1 PUT and 10 mgL−1 STS, resulting in fast-growing plantlets during acclimatization phase, reaching 90% of plant survival. The genetic fidelity assessment of plants derived from micropropagation was confirmed by RAPD analysis. Four operon primers were used, and all of them showed amplified unambiguous (OPA02, OPC-04, OPD-07, and OPE-15). All generated bands were monomorphic and had no variation among the tissue culture-derived plants tested. Accordingly, these results indicate that adding polyamines and silver thiosulfate to the nutrient medium of date palm cv. Quntar is beneficial in improving shoot organogenesis, rooting, and production of genetically stable date palm plants.

Download Full-text

65 years of in vitro culture in Poland

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2013.022 ◽

2013 ◽

Vol 82 (3) ◽

pp. 183-192

Author(s):

Maciej Zenkteler ◽

Elżbieta Zenkteler

Keyword(s):

Tissue Culture ◽

In Vitro Culture ◽

Plant Disease Resistance ◽

Culture Method ◽

Short Review ◽

Meristem Culture ◽

Biological Processes ◽

Practical Applications ◽

Tissue Culture Method

This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications, have been achieved within the areas of meristem culture and micropropagation of valuable genotypes, somaclonal variation, production of haploids and DH plants, somatic embryogenesis, in vitro culture of embryos and ovules, improvement of plant disease resistance, somatic hybridization and transformation of plants. Due to the fact that in vitro domain is a very broad science the authors are aware that this review might not fully cover all the scientists working with tissue culture and their achievements.

Download Full-text

RAPID IN VITRO PROPAGATION OF WESTERN GHATS CULTIVAR - COLOCASIA ESCULENTA FOR THE HIGH FREQUENCY OF PLANTLETS

Journal of Drug Discovery and Therapeutics ◽

10.32553/jddt.v7i2.447 ◽

2019 ◽

Vol 7 (2) ◽

Author(s):

Jyothi R ◽

Srinivasa Murthy K M ◽

Hossein . ◽

Veena .

Keyword(s):

Tissue Culture ◽

Wide Distribution ◽

Colocasia Esculenta ◽

Limiting Factor ◽

Promising Alternative ◽

Rapid Multiplication ◽

Medical Plant ◽

Total Fat ◽

Planting Materials

Colocasia esculenta is commonly known as Taro, it is referred to as cocoyam in Nigeria. They are cherished for their rich taste, nutritional and medicinal properties. Every 100 g of taro corms possess 112 Kcal, 26.46 g carbohydrate, 1.50 g protein, 0.20 g total fat and 4.1g fiber (USDA National Nutrient Data Base). Besides its nutritional value, taro is used as a medical plant and provides bioactive compounds used as an anti-cancer drugs. Traditionally, cocoyams are vegetative propagated from tuber fragments, a practice that encourages pathogen distribution. Colocasia esculenta is a widely distributed food crop in the humid tropics and subtropics. Despite of its wide distribution, Taro plants are commonly infected with DsMV and other pathogens. This virus induces conspicuous mosaic, malformation, dwarfing or feathering on leaves in taro. As the results of infection, it reduces the quality and yield of taro production greatly. This virus is thus considered as a major limiting factor in the production of taro. Here plays the importance of tissue culture plays a major role in producing the disease resistant plants round the year with high quality. For rapid multiplication and production of quality planting materials, tissue culture technology offers promising alternative compared to the traditional production methods. KEYWORDS: Colocasia esculenta, Virus, Pathogens, Conventional propagation, Micropropagation, Yield, Rapid multiplication, Quality

Download Full-text

STUDIES ON THE GROWTH OF EXPERIMENTAL OVARIAN TUMOURS IN TISSUE CULTURE

Acta Endocrinologica ◽

10.1530/acta.0.xxxii0041 ◽

1959 ◽

Vol XXXII (I) ◽

pp. 41-53 ◽

Cited By ~ 8

Author(s):

Stig Kullander ◽

Bengt Källén

Keyword(s):

Tissue Culture ◽

Granulosa Cell ◽

In Vitro Study ◽

Vitro Study ◽

Ovarian Tumours

ABSTRACT An in vitro study has been made of experimentally produced rat ovarian tumours of different age, paying particular attention to tumour reaction to crystallized steroids. Tumours of two histological structures were found: granulosa cell – luteoma tumours and arrhenoblastoma tumours. Both types grew in vitro and pictures of their cell appearance are given. The former type gave the best growth, and the endocrine studies were restricted to this type. The steroids tested (androsterone, oestrone, progesterone) all had an arresting effect in certain cases. This effect is not an unspecific, toxic one. The different tumours react to different extents, some being completely unaffected.

Download Full-text