scholarly journals Biochemical and Immunological Characterization of Collagenase in Tissues of Metamorphosing Bullfrog Tadpoles. (collagenase/bullfrog tadpole/thyroid hormone/metamorphosis/TIMP)

1991 ◽  
Vol 33 (4) ◽  
pp. 329-339 ◽  
Author(s):  
Ken Oofusa ◽  
Katsutoshi Yoshizato
Keyword(s):  
Thyroid Hormone ◽  
Immunological Characterization ◽  
Bullfrog Tadpole

The Immunological Characterization of Human Antibodies to Factor VIII Isolated by Immuno-Affinity Chromatography

1981 ◽  
Vol 45 (01) ◽  
pp. 060-064 ◽  
Author(s):  
M L Kavanagh ◽  
C N Wood ◽  
J F Davidson
Keyword(s):  
Factor Viii ◽  
Affinity Chromatography ◽  
Gel Filtration ◽  
Immunological Characterization ◽  
Human Antibodies ◽  
Heavy Chains ◽  
Light Chain Type ◽  
Antibody Preparations ◽  
Chain Type

SummaryNine human antibodies to factor VIII were isolated from haemophilic plasmas by affinity chromatography and gel filtration and six were subsequently subjected to immunological characterization. Three partially purified preparations were similarly characterized. Eight of the antibodies were characterized as being exclusively IgG and one preparation was found to contain IgM. Seven of the antibodies contained only a single light chain type, four being of type lambda and three of type kappa. Two antibody preparations contained both kappa and lambda light chains. In four of the preparations, only a single heavy chain sub-class could be demonstrated, three of IgG3 and one of IgG4. Of the remainder, three were a mixture of IgG3 and IgG4 sub-classes and one contained both IgG2 and IgG4. IgG sub-classification could not be achieved with the IgM-containing preparation. These results demonstrate a restricted heterogeneity of light and heavy chains in human antibodies to factor VIII.

scholarly journals Generation and Characterization of dickkopf3 Mutant Mice

2006 ◽  
Vol 26 (6) ◽  
pp. 2317-2326 ◽  
Author(s):  
Ivan del Barco Barrantes ◽  
Ana Montero-Pedrazuela ◽  
Ana Guadaño-Ferraz ◽  
Maria-Jesus Obregon ◽  
Raquel Martinez de Mena ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Clinical Chemistry ◽  
Lung Ventilation ◽  
Immunoglobulin M ◽  
Mutant Mice ◽  
Phenotypic Analysis ◽  
Thyroid Hormone Metabolism ◽  
Hormone Binding Protein ◽  
Deficient Mice

ABSTRACT dickkopf (dkk) genes encode a small family of secreted Wnt antagonists, except for dkk3, which is divergent and whose function is poorly understood. Here, we describe the generation and characterization of dkk3 mutant mice. dkk3-deficient mice are viable and fertile. Phenotypic analysis shows no major alterations in organ morphology, physiology, and most clinical chemistry parameters. Since Dkk3 was proposed to function as thyroid hormone binding protein, we have analyzed deiodinase activities, as well as thyroid hormone levels. Mutant mice are euthyroid, and the data do not support a relationship of dkk3 with thyroid hormone metabolism. Altered phenotypes in dkk3 mutant mice were observed in the frequency of NK cells, immunoglobulin M, hemoglobin, and hematocrit levels, as well as lung ventilation. Furthermore, dkk3-deficient mice display hyperactivity.

scholarly journals Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2

Virology ◽  
2021 ◽  
Vol 557 ◽  
pp. 15-22
Author(s):  
Teodora Djukic ◽  
Maja Mladenovic ◽  
Dragana Stanic-Vucinic ◽  
Jelena Radosavljevic ◽  
Katarina Smiljanic ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Protein Fragment ◽  
Immunological Characterization ◽  
Recombinant Nucleocapsid Protein

Genetic and immunological characterization of naturally occurring recombinant B3 rats

1977 ◽  
Vol 5 (1) ◽  
pp. 271-283 ◽  
Author(s):  
Heinz W. Kunz ◽  
Thomas J. Gill ◽  
Barbara Dixon ◽  
John W. Shonnard ◽  
Bridgett K. Davis ◽  
...  
Keyword(s):  
Immunological Characterization ◽  
Naturally Occurring
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