ULTRASTRUCTURAL OBSERVATION OF NEONATAL LUNGS WITH TRANSMISSION ELECTRON MICROSCOPE AND FREEZE-FRACTURE REPLICATION

1981 ◽  
Vol 31 (6) ◽  
pp. 979-997
Author(s):  
Toshiaki Manabe
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Freeze Fracture ◽  
Ultrastructural Observation ◽  
Transmission Electron ◽  
Neonatal Lungs

scholarly journals A Novel Microchip Technique for Quickly Identifying Nanogranules in an Aqueous Solution by Transmission Electron Microscopy: Imaging of Platelet Granules

2020 ◽  
Vol 10 (14) ◽  
pp. 4946
Author(s):  
Nguyen Thi Thu Trang ◽  
Jungshan Chang ◽  
Wei-An Chen ◽  
Chih-Chun Chen ◽  
Hui-Min Chen ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Aqueous Solution ◽  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Human Platelet ◽  
Blood Collection ◽  
Ultrastructural Observation ◽  
Microscopy Imaging ◽  
Transmission Electron ◽  
Aqueous Conditions

Ultrastructural observation of biological specimens or nanogranules usually requires the use of electron microscopy. Electron microscopy takes a lot of time, requires many steps, and uses many chemicals, which may affect the native state of biological specimens. A novel microchip (K-kit) was used as a specimen kit for in situ imaging of human platelet granules in an aqueous solution using a transmission electron microscope. This microchip enabled us to observe the native human platelet granules very quickly and easily. The protocols included blood collection, platelet purification, platelet granule isolation, sample loading into this microchip, and then observation by a transmission electron microscope. In addition, these granules could still remain in aqueous solution, and only a very small amount of the sample was required for observation and analysis. We used this microchip to identify the native platelet granules by negative staining. Furthermore, we used this microchip to perform immunoelectron microscopy and successfully label α-granules of platelets with the anti-P-selectin antibody. These results demonstrate that the novel microchip can provide researchers with faster and better choices when using a transmission electron microscope to examine nanogranules of biological specimens in aqueous conditions.

Ultrastructure of ascus and ascospore appendages of the mangrove fungus Halosarpheia ratnagiriensis (Halosphaeriales, Ascomycota)

2001 ◽  
Vol 79 (11) ◽  
pp. 1307-1317 ◽  
Author(s):  
T A Baker ◽  
E BG Jones ◽  
S T Moss
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Freeze Fracture ◽  
Transmission Electron ◽  
Mucilaginous Material

Halosarpheia Kohlm. et E. Kohlm is a genus of eighteen species, all of which have septate ascospores with unfurling polar appendages. Asci and ascospores of Halosarpheia ratnagiriensis Patil et Borse were examined at the scanning (including freeze-fracture) and transmission electron microscope levels. The ascus wall has two well-defined layers and the apical apparatus comprises a refractive, electron-dense, lens-shaped disk embedded within a less electron-dense thickening. The ascospore wall comprises an episporium and a bipartite mesosporium, and the appendages are formed by extrusion of mucilaginous material through an episporial pore field. Ascospore appendage ontogeny is compared with other genera with unfurling polar appendages: Cataractispora, Diluviocola, Tunicatispora, Tirispora, and Halosarpheia aquadulcis Hsieh, H.S. Chang et E.B.G. Jones and Halosarpheia heteroguttulata S.W. Wong, K.D. Hyde et E.B.G. Jones.Key Words: ascospore appendage ontogeny, marine ascomycetes, unfurling polar appendages, ultrastructure.

Freeze-Fracture Demonstration of a Suture in the Free Sporozoite of Plasmodium Berghei

1982 ◽  
Vol 40 ◽  
pp. 314-315
Author(s):  
Charles A.M. Meszoely ◽  
Eric F. Erbe ◽  
Russell L. Steere ◽  
Nancy D. Pacheco ◽  
Richard L. Beaudoin
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Plasmodium Berghei ◽  
Phosphate Buffer ◽  
Sodium Phosphate ◽  
Anopheles Stephensi ◽  
Freeze Fracture ◽  
Sodium Phosphate Buffer ◽  
Transmission Electron ◽  
Cell Components

The ANKA strain of Plasmodium berghei was maintained in Anopheles Stephensi mosquitoes. Free sporozoites were obtained from a homogenized suspension of mosquito thoraces and heads. They were separated from cell components on a discontinuous gradient, concentrated by centrifugation, fixed in 2% glutaraldehyde, and cryoprotected in a solution containing approximately 12.5% glycerol by volume, and 12.5% sucrose by weight in a 0.2 molar sodium phosphate buffer. The sporozoite preparation was freeze-etched for 1 minute in a modified Denton DFE-2 freeze-etch module. The replicas were photographed in stereo (10 tilt between micrograph pairs) with a JEM-100B transmission electron microscope equipped with a 60° top entry goniometer stage.

scholarly journals Down the Resolution Road: Freeze-Fracture Revisited?

1995 ◽  
Vol 3 (1) ◽  
pp. 3-4
Author(s):  
Stephen W. Carmichael
Keyword(s):  
Electron Microscope ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Transmission Electron Microscope ◽  
Freeze Fracture ◽  
Plant Viruses ◽  
Transmission Electron ◽  
Scalpel Blade

It must have seemed rather fantastic back in the fifties, when Russell Steere froze chunks of plant viruses (tobacco mosaic virus, tobacco nngspot virus, and squash mosaic virus) in drops of water, planed them freehand with a scalpel blade, made a replica of the surface, and examined the replica in a transmission electron microscope. But that was the birth of freeze-fracture and freeze-etch methodology that yielded enormous amounts of information about the morphology of membranes.

A Reproducible Selective Stain for Cardiac Sarcoplasmic Reticulum

1974 ◽  
Vol 32 ◽  
pp. 214-215
Author(s):  
R. A. Waugh ◽  
J. R. Sommer
Keyword(s):  
Complex System ◽  
Electron Microscope ◽  
Sarcoplasmic Reticulum ◽  
Transmission Electron Microscope ◽  
Electron Microscopic ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Transmission Electron

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.

Surface Structure of the Spores of Glugea Weissenbergi

1969 ◽  
Vol 27 ◽  
pp. 238-239
Author(s):  
Sanford H. Vernick ◽  
Anastasios Tousimis ◽  
Victor Sprague
Keyword(s):  
Electron Microscope ◽  
Surface Structure ◽  
Transmission Electron Microscope ◽  
Mature Spore ◽  
Spore Surface ◽  
Sectioned Material ◽  
Transmission Electron ◽  
Surface Detail

Recent electron microscope studies have greatly expanded our knowledge of the structure of the Microsporida, particularly of the developing and mature spore. Since these studies involved mainly sectioned material, they have revealed much internal detail of the spores but relatively little surface detail. This report concerns observations on the spore surface by means of the transmission electron microscope.

A New High Resolution Transmission Electron Microscope with Second-Zone Objective Lens

1969 ◽  
Vol 27 ◽  
pp. 176-177 ◽  
Author(s):  
H. Tochigi ◽  
H. Uchida ◽  
S. Shirai ◽  
K. Akashi ◽  
D. J. Evins ◽  
...  
Keyword(s):  
Electron Microscope ◽  
High Resolution ◽  
Transmission Electron Microscope ◽  
Objective Lens ◽  
High Excitation ◽  
Transmission Electron ◽  
New Instrument

A New High Excitation Objective Lens (Second-Zone Objective Lens) was discussed at Twenty-Sixth Annual EMSA Meeting. A new commercially available Transmission Electron Microscope incorporating this new lens has been completed.Major advantages of the new instrument allow an extremely small beam to be produced on the specimen plane which minimizes specimen beam damages, reduces contamination and drift.

X-ray microanalysis of thin specimens in the transmission electron microscope at voltages up to 1000 Kv.

1978 ◽  
Vol 36 (1) ◽  
pp. 540-541
Author(s):  
G. Cliff ◽  
M.J. Nasir ◽  
G.W. Lorimer ◽  
N. Ridley
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Weight Fraction ◽  
Present Series ◽  
Constant Independent ◽  
X Ray ◽  
Transmission Electron ◽  
Series Of Experiments ◽  
Image Position ◽  
X Ray Absorption

In a specimen which is transmission thin to 100 kV electrons - a sample in which X-ray absorption is so insignificant that it can be neglected and where fluorescence effects can generally be ignored (1,2) - a ratio of characteristic X-ray intensities, I1/I2 can be converted into a weight fraction ratio, C1/C2, using the equationwhere k12 is, at a given voltage, a constant independent of composition or thickness, k12 values can be determined experimentally from thin standards (3) or calculated (4,6). Both experimental and calculated k12 values have been obtained for K(11<Z>19),kα(Z>19) and some Lα radiation (3,6) at 100 kV. The object of the present series of experiments was to experimentally determine k12 values at voltages between 200 and 1000 kV and to compare these with calculated values.The experiments were carried out on an AEI-EM7 HVEM fitted with an energy dispersive X-ray detector.

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