Prostate-specific antigen assay using whole blood samples spotted on filter paper and its application to mass screening for prostate cancer

2007 ◽  
Vol 14 (6) ◽  
pp. 505-509 ◽  
Author(s):  
Masaharu Nanri ◽  
Kazushige Nanri ◽  
Chisato Fujiyama ◽  
Yuji Tokuda ◽  
Kiyoko Nakamura ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Mass Screening ◽  
Filter Paper ◽  
Whole Blood ◽  
Specific Antigen ◽  
Blood Samples ◽  
Antigen Assay ◽  
Whole Blood Samples

scholarly journals Assay of prostate-specific antigen from whole blood spotted on filter paper and application to prostate cancer screening

1996 ◽  
Vol 42 (4) ◽  
pp. 536-544 ◽  
Author(s):  
B R Hoffman ◽  
H Yu ◽  
E P Diamandis
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Filter Paper ◽  
Whole Blood ◽  
Large Scale ◽  
Prostate Cancer Screening ◽  
Specific Antigen ◽  
Blood Concentrations ◽  
Hot Climate ◽  
Plasma Fraction

Abstract We report the measure of prostate-specific antigen (PSA) from extracts of blood dried on filter paper. Five 3-mm (diameter) paper discs containing approximately 25 microL of dried whole blood were punched from the filter paper and extracted with 500 microL of buffer. Recovery of PSA was > 92%. Imprecision of the filter paper procedure was <10% when corresponding whole-blood concentrations were >0.35 micrograms/L. PSA recovery was unaffected whether blood was applied to the filter as one 85-microL aliquots, two 43-microL aliquots, or three 28-microL aliquots. PSA is contained in the plasma fraction. Variation in hematocrit from 0.61 to 0.31 caused <+/-10% change in filter paper PSA. Regression analysis showed: filter paper PSA = 0.86 whole-blood PSA - 0.02; Sy/x = 0.44. Men (153) without prostate cancer gave a 95th percentile of 4.8 micrograms /L. PSA in filter paper dried blood was stable for >1 month at -20 to 37 degrees C and showed no loss of recovery after being mailed to a hot climate. We conclude that the filter paper procedure can reliably distinguish normal from increased concentrations of PSA and that it could facilitate screening to detect occult prostate cancer in large-scale mail-in programs to centralized laboratories.

scholarly journals Measurement of Circulating Forms of Prostate-specific Antigen in Whole Blood Immediately after Venipuncture: Implications for Point-of-Care Testing

2001 ◽  
Vol 47 (4) ◽  
pp. 703-711 ◽  
Author(s):  
Timo Piironen ◽  
Martti Nurmi ◽  
Kerttu Irjala ◽  
Olli Heinonen ◽  
Hans Lilja ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Prostate Specific Antigen ◽  
Whole Blood ◽  
Point Of Care ◽  
Prostate Gland ◽  
Specific Antigen ◽  
Point Of Care Testing ◽  
Blood Samples ◽  
Whole Blood Samples

Abstract Background: The purpose of this study was to validate the use of whole-blood samples in the determination of circulating forms of prostate-specific antigen (PSA). Methods: Blood samples of hospitalized prostate cancer and benign prostatic hyperplasia patients were collected and processed to generate whole-blood and serum samples. Three different rapid two-site immunoassays were developed to measure the concentrations of total PSA (PSA-T), free PSA (PSA-F), and PSA-α1-antichymotrypsin complex (PSA-ACT) to detect in vitro changes in whole-blood samples immediately after venipuncture. The possible influence of muscle movement on the release of PSA from prostate gland was studied in healthy men by measuring the rapid in vitro whole-blood kinetics of PSA forms before and after 15 min of physical exercise on a stationary bicycle. Results: Rapid PSA-T, PSA-F, and PSA-ACT assays were designed using a 10-min sample incubation. No significant changes were detected in the concentrations of PSA-T, PSA-F, and PSA-ACT from the earliest time point of 12–16 min compared with measurements performed up to 4 h after venipuncture. Physical exercise did not influence the concentrations of the circulating forms of PSA. Hematocrit-corrected whole-blood values of PSA-T and PSA-F forms were comparable to the respective serum values. Calculation of the percentage of PSA-F (PSA F/T ratio × 100) was similar irrespective of the sample format used, i.e., whole blood or serum. Conclusions: We found that immunodetectable PSA forms are likely at steady state immediately after venipuncture, thus enabling the use of anticoagulated whole-blood samples in near-patient settings for point-of-care testing, whereas determinations of PSA (e.g., PSA-T, PSA-F, or PSA-ACT) performed within the time frame of the office visit would provide results equivalent to conventional analyses performed in serum.

scholarly journals Measurement of Prostate-specific Antigen by Use of a Novel Blood Collection and Analytical System

2002 ◽  
Vol 48 (8) ◽  
pp. 1272-1278 ◽  
Author(s):  
Barbara R Grzeda ◽  
Tuan Le Bui ◽  
Cheryl N Warner ◽  
Tracy L Pirucki ◽  
Lisa M Dewey ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Whole Blood ◽  
Prostate Cancer Screening ◽  
Specific Antigen ◽  
Blood Collection ◽  
Serum Samples ◽  
Stabilizing Solution ◽  
Professional Assistance ◽  
Comparison Of The Results

Abstract Background: Prostate-specific antigen (PSA) is widely used in the detection and monitoring of prostate cancer. We developed a system for the self-collection and transport of capillary whole blood for PSA analysis, with the goal of reducing phlebotomy visits and, thus, increasing the access and utilization of PSA in prostate cancer screening and monitoring. Methods: The blood collection device [BIOSAFE Blood Transport System (BTSTM)] collects 70 μL of blood through a heparin-coated material into 200 μL of stabilizing solution. The diluted whole blood is used for measurement of PSA by a modified version of the Hybritech® Tandem-MP PSA Assay. Results were compared for matched samples of professionally and self-collected BTS blood and for matched BTS samples sera from blood collected by venipuncture. Imprecision for the whole-blood PSA measurement was estimated from analysis of whole-blood controls in duplicate, twice per day, over 20 days. Results: BTS samples (n = 140) collected by a qualified healthcare professional compared with serum samples yielded the regression equation: y =1.02x + 0.04 (Sy|x = 0.35; r = 0.99). Comparison of the results for samples (n = 128) collected by the patient without professional assistance with serum samples yielded: y = 1.08x + 0.02 (Sy|x = 0.31; r = 0.99). The between-run CVs at 0.069, 0.53, 2.9, and 10.7 μg/L were 21%, 6.0%, 3.5%, and 3.8%, respectively. PSA was stable in BTS samples stored for 21 days at 18–24 °C and for 7 days at 37 °C. Conclusion: The BIOSAFE BTS system allows accurate and convenient measurement of circulating PSA by a precise method for diluted whole blood.

scholarly journals Simultaneous Quantification of Prostate-specific Antigen and Human Glandular Kallikrein 2 mRNA in Blood Samples from Patients with Prostate Cancer and Benign Disease

2002 ◽  
Vol 48 (8) ◽  
pp. 1265-1271 ◽  
Author(s):  
Alice Ylikoski ◽  
Kim Pettersson ◽  
Jussi Nurmi ◽  
Kerttu Irjala ◽  
Matti Karp ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Benign Disease ◽  
Early Event ◽  
Blood Samples ◽  
Simultaneous Quantification ◽  
Glandular Kallikrein ◽  
External Calibration Curve ◽  
Kallikrein 2

Abstract Background: Detection or quantification of circulating cancer cells has been proposed as an aid in detection and monitoring of several solid tumors. We investigated the classification accuracy of prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2) mRNA copy numbers in blood for the differentiation of patients with prostate cancer (PC) and benign disease. Methods: PSA and hK2 mRNA expression was studied in blood samples from 51 men with PC and 19 men with benign disease. Among the PC patients, 10 had organ-confined disease (pT1–pT2). We used a multiplexed reverse transcription-PCR assay with two highly target-like mRNA internal standards for the simultaneous quantification of PSA and hK2 mRNA. An external calibration curve covered the range of 102–106 mRNA copies. Results: PSA and hK2 mRNA were detected in 41 of 51 (median, 1200 copies/0.5 mL of blood) and 43 of 51 (median, 3800 copies/0.5 mL of blood) patients with PC, respectively, whereas only 1 of 19 men with benign disease was positive for both mRNAs (1500 PSA and 3100 hK2 mRNA copies/0.5 mL of blood; P <0.0001, Mann–Whitney U-test). Of the 10 patients with organ-confined PC, only 3 with low Gleason scores (≤5) were negative for both PSA and hK2 (P = 0.02, Mann–Whitney U-test). Conclusions: The presence of PC cells in the blood circulation is an early event in PC progression, and quantitative assays for PSA and hK2 mRNA discriminate benign from PC cases. Further studies are needed to determine the diagnostic accuracy and prognostic value of the assays.

PD53-07 CLINICAL SIGNIFICANCE OF THE SIAα2,3GAL-GLYCOSYLATED PROSTATE-SPECIFIC ANTIGEN ASSAY FOR PROSTATE CANCER DETECTION

2020 ◽  
Vol 203 ◽  
pp. e1099
Author(s):  
Tohru Yoneyama* ◽  
Yuki Tobisawa ◽  
Tomokazu Ishikawa ◽  
Shingo Hatakeyama ◽  
Kazuyuki Mori ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Clinical Significance ◽  
Cancer Detection ◽  
Specific Antigen ◽  
Prostate Cancer Detection ◽  
Antigen Assay

scholarly journals Clinical significance of the Lacdi NA c‐glycosylated prostate‐specific antigen assay for prostate cancer detection

2019 ◽  
Vol 110 (8) ◽  
pp. 2573-2589 ◽  
Author(s):  
Tohru Yoneyama ◽  
Yuki Tobisawa ◽  
Tomonori Kaneko ◽  
Takatoshi Kaya ◽  
Shingo Hatakeyama ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Clinical Significance ◽  
Cancer Detection ◽  
Specific Antigen ◽  
Prostate Cancer Detection ◽  
Antigen Assay

An Automated Procedure for Blood Phenylalanine

1965 ◽  
Vol 11 (5) ◽  
pp. 541-546 ◽  
Author(s):  
John B Hill ◽  
George K Summer ◽  
Murray W Pender ◽  
Norris O Roszel
Keyword(s):  
Filter Paper ◽  
Whole Blood ◽  
Blood Samples ◽  
Blood Phenylalanine ◽  
Large Populations ◽  
Fluorometric Analysis ◽  
Whole Blood Samples

Abstract The fluorometric analysis of phenylalanine described by McCaman and Robins has been adapted to automation. The procedure may be used on 20-µl. whole-blood samples which may be stored on filter paper for elution. The procedure is rapid, reproducible, and accurate, making it useful for screening large populations for phenylketonuria.

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