STICKY ENDS

2014 ◽  
Vol 25 (4) ◽  
pp. 7-7
Author(s):  
Keyvan Vakili
Keyword(s):  
Sticky Ends

Synthesis and Characterization of Oligodeoxynucleotides Containing Naphthyridine:Imidazopyridopyrimidine Base Pairs at their Sticky Ends. Application as Thermally Stabilized Decoy Molecules

ChemBioChem ◽  
2006 ◽  
Vol 7 (12) ◽  
pp. 1970-1975 ◽  
Author(s):  
Sadao Hikishima ◽  
Noriaki Minakawa ◽  
Kazuyuki Kuramoto ◽  
Shintaro Ogata ◽  
Akira Matsuda
Keyword(s):  
Synthesis And Characterization ◽  
Base Pairs ◽  
Sticky Ends

Directional cloning of native PCR products with preformed sticky ends (Autosticky PCR)

1999 ◽  
Vol 260 (6) ◽  
pp. 569-573 ◽  
Author(s):  
J. Gál ◽  
R. Schnell ◽  
S. Szekeres ◽  
M. Kálmán
Keyword(s):  
Directional Cloning ◽  
Pcr Products ◽  
Sticky Ends

Realizing directional cloning using sticky ends produced by 3′-5′ exonuclease of Klenow fragment

2013 ◽  
Vol 38 (5) ◽  
pp. 857-866 ◽  
Author(s):  
Guojie Zhao ◽  
Jun Li ◽  
Tianyu Hu ◽  
Hua Wei ◽  
Yifu Guan
Keyword(s):  
Klenow Fragment ◽  
Directional Cloning ◽  
Sticky Ends

Charge transfer in DNA assemblies: effects of sticky ends

2008 ◽  
pp. 2656 ◽  
Author(s):  
Yasuko Osakada ◽  
Kiyohiko Kawai ◽  
Mamoru Fujitsuka ◽  
Tetsuro Majima
Keyword(s):  
Charge Transfer ◽  
Sticky Ends ◽  
Charge Transfer In Dna

scholarly journals Fabrication of DNA nanotubes using origami-based nanostructures with sticky ends

2015 ◽  
Vol 5 (2) ◽  
pp. 177-183 ◽  
Author(s):  
Mohammad Mousavi-Khattat ◽  
Adele Rafati ◽  
Pooria Gill
Keyword(s):  
Sticky Ends ◽  
Dna Nanotubes

scholarly journals Reorganization of Self-Assembled DNA-Based Polymers Using Orthogonally Addressable Building Blocks

2021 ◽  
Author(s):  
Serena Gentile ◽  
Erica Del Grosso ◽  
Leonard J. Prins ◽  
Francesco Ricci
Keyword(s):  
Self Assembly ◽  
Rational Design ◽  
Building Blocks ◽  
Binding Domain ◽  
Non Covalent Interactions ◽  
Sticky Ends ◽  
Self Assembled ◽  
Block Structures ◽  
Covalent Interactions

Taking advantage of the addressability and programmability of DNA/DNA non-covalent interactions we report here the rational design of orthogonal DNA-based addressable tiles that self-assemble into polymer-like structures that can be reconfigured and reorganized by external inputs. The different tiles share the same 5-nucleotide sticky ends responsible for self-assembly but are rationally designed to contain a specific regulator-binding domain that can be orthogonally targeted by different DNA regulator strands (activators and inhibitors). We show that by sequentially adding specific activators and inhibitors it is possible to re-organize in a dynamic and reversible way the formed polymer-like structures to display well-defined distributions: homopolymers made of a single tile, random polymers in which different tiles are distributed randomly and block structures in which the tiles are organized in segments.

scholarly journals A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions.

1997 ◽  
Vol 44 (4) ◽  
pp. 849-852 ◽  
Author(s):  
M Kawalec ◽  
P Borsuk ◽  
S Piechula ◽  
P P Stepień
Keyword(s):  
Low Temperature ◽  
Restriction Endonuclease ◽  
Bacterial Strain ◽  
Inorganic Phosphate ◽  
Restriction Enzymes ◽  
Type Ii ◽  
Temperature Optimum ◽  
Phosphate Ions ◽  
Sticky Ends

A novel type II restriction endonuclease UnbI was isolated from an unidentified psychrofilic bacterial strain from Antarctica. UnbI recognizes and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long sticky ends. In this respect it differs from its neoschizomer Sau96I and all other restriction enzymes recognizing this sequence. UnbI has a relatively low temperature optimum of 15 degrees C to 20 degrees C and its activity is completely inhibited by inorganic phosphate.

scholarly journals DNA bioelectric field: a futuristic bioelectric marker of cancer, aging and death – A working hypothesis

2021 ◽  
Vol 3 (1) ◽  
pp. 68-79
Author(s):  
Matti Pitkänen ◽  
◽  
Reza Rastmanesh ◽  
Keyword(s):  
Magnetic Flux ◽  
Electric Field ◽  
Longitudinal Electric Field ◽  
Magnetic Flux Tube ◽  
Flux Tubes ◽  
Dna Strands ◽  
Standard Picture ◽  
Magnetic Flux Tubes ◽  
Sticky Ends ◽  
The Difference

Telomeres are associated with the ends of DNA double strands. The lengths of the telomeres are controlled by the telomerase enzyme. The shortening of the telomeres is known to relate to aging. In cancers, telomere lengths are abnormally short. Telomeres could act as buffers shielding the part of DNA coding for the proteins. For cancer cells, germ cells and stem cells the length of the telomeres is not varying. There is an analogy with microtubules, which are highly dynamical and carry a longitudinal electric field, whose strength correlates with the microtubule length. Could sticky ends generate a longitudinal field along DNA double strand with strength determined by the lengths of the sticky ends? In the standard picture the flux of the longitudinal electric field would be proportional to the difference of the negative charges associated with the sticky ends. In TGD framework, DNA strands are accompanied by the dark analog of DNA with codons realized as 3-proton units at magnetic flux tubes parallel to DNA strands and neutralizing the negative charge of ordinary DNA except at the sticky ends. This allows considering the possibility that opposite sticky ends carry opposite charges generating a longitudinal electric field along the magnetic flux tube associated with the system. DNA/Telomere bioelectric field could serve as a novel bioelectric marker to be used for prognostic and diagnostic purposes in researches of cancer, aging, surgery grafts and rejuvenation. We propsed that DNA bioelectric field can be used as a futuristic bioelectric marker of cancer, aging and death.

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