scholarly journals Genetic relatedness between group B streptococci originating from bovine mastitis and a human group B streptococcus type V cluster displaying an identical pulsed-field gel electrophoresis pattern

2006 ◽  
Vol 12 (9) ◽  
pp. 887-893 ◽  
Author(s):  
I.C.M. Oliveira ◽  
M.C. de Mattos ◽  
T.A. Pinto ◽  
B.T. Ferreira-Carvalho ◽  
L.C. Benchetrit ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Genetic Relatedness ◽  
Group B Streptococcus ◽  
Bovine Mastitis ◽  
Pulsed Field Gel Electrophoresis ◽  
Human Group ◽  
Group B Streptococci ◽  
Pulsed Field ◽  
Group B ◽  
Type V

Pulsed-Field Gel Electrophoresis of Human Group B Streptococci Isolated in Brazil

2005 ◽  
Vol 17 (3) ◽  
pp. 258-263 ◽  
Author(s):  
I.C.M. Oliveira ◽  
M.C. De mattos ◽  
M.F.T. Areal ◽  
B.T. Ferreira-carvalho ◽  
A.M.S. Figueiredo ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Human Group ◽  
Group B Streptococci ◽  
Pulsed Field ◽  
Group B

scholarly journals Sudden Increase in Isolation of Group B Streptococci, Serotype V, Is Not Due to Emergence of a New Pulsed-Field Gel Electrophoresis Type

1998 ◽  
Vol 36 (7) ◽  
pp. 2115-2116 ◽  
Author(s):  
J. A. Elliott ◽  
K. D. Farmer ◽  
R. R. Facklam
Keyword(s):  
Gel Electrophoresis ◽  
Group B Streptococcus ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Sudden Increase ◽  
Chromosomal Dna ◽  
Group B Streptococci ◽  
Pulsed Field ◽  
Group B ◽  
Human Infections

Until recently, group B streptococcus, serotype V (GBS-V), was an infrequent cause of disease. It is now recognized as a significant cause of infections in both children and adults. To determine if this increase was due to the recent introduction and spread of a single clone of GBS-V, we analyzed, by pulsed-field gel electrophoresis (PFGE), the SmaI chromosomal DNA digests of 45 bacteria: 41 isolated from human infections between 1986 and 1996 in the United States, 2 from human infections in Argentina, and 2 from naturally infected mice. Seventeen patterns were found and arbitrarily designated patterns A to Q. Pattern N constituted 24 (53%) of the isolates and was found in all of the years tested and from all surveillance areas, as well as in both isolates from Argentina, and was very similar to the GBS-V isolated from a mouse. Pattern P was found in three isolates, pattern F was found in two, and the remaining patterns were found in one isolate each. We concluded that the majority of isolates of GBS-V are of one PFGE subtype and that this subtype was predominate before the increase in disease caused by GBS-V and that GBS-V disease is caused by several different subtypes.

scholarly journals Pulsed Field Gel Electrophoresis Analysis Conditions for Molecular Epidemiological Identification of Vertical Group B Streptococcus Infection

2005 ◽  
Vol 79 (8) ◽  
pp. 561-563
Author(s):  
Hiroko WAKIMOTO ◽  
Hisako YANO ◽  
Shigeyoshi BABA ◽  
Katsuko OKUZUMI ◽  
Noriko OKAMOTO ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Group B Streptococcus ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Group B

scholarly journals Pulsed Field Gel Electrophoresis Analysis Conditions for Molecular Epidemiological Group B Streptococcus Identification

2008 ◽  
Vol 82 (4) ◽  
pp. 351-353
Author(s):  
Hiroko WAKIMOTO ◽  
Masamichi OGURA ◽  
Hisako YANO ◽  
Akira KOGUCHI ◽  
Yukio WAKIMOTO ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Group B Streptococcus ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Group B

scholarly journals Distribution of Antimicrobial Resistance and Virulence-Related Genes among Brazilian Group B Streptococci Recovered from Bovine and Human Sources

2005 ◽  
Vol 49 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Rafael S. Duarte ◽  
Bruna C. Bellei ◽  
Otávio P. Miranda ◽  
Maria A. V. P. Brito ◽  
Lúcia M. Teixeira
Keyword(s):  
Genetic Diversity ◽  
Antimicrobial Resistance ◽  
Streptococcus Agalactiae ◽  
Gel Electrophoresis ◽  
Present Report ◽  
Pulsed Field Gel Electrophoresis ◽  
Group B Streptococci ◽  
Pulsed Field ◽  
Group B

ABSTRACT In the present report we describe the characteristics of 189 antimicrobial-resistant Streptococcus agalactiae isolates from bovine (38 isolates) and human (151 isolates) sources. All the strains were resistant to tetracycline (TET), and 16 (8.5%) were also resistant to erythromycin, corresponding to 23.7% of the TET-resistant bovine isolates and 4.6% of the TET-resistant human isolates. The tet(O), erm(B), and mreA resistance-related genes, as well as the bca and scpB virulence-related genes, were the most frequent among the bovine isolates, while the tet(M), erm(A), mreA, bca, lmb, and scpB genes were the most prevalent among the isolates from humans. Although a few major clusters were observed, pulsed-field gel electrophoresis results revealed a variety of profiles, reflecting the substantial genetic diversity among strains of this species isolated from either humans or bovines.

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