Molecular detection of Rickettsia typhi and Rickettsia felis in fleas from Algeria

Rickettsia typhi is the causal agent of murine typhus; a worldwide zoonotic and vector-borne infectious disease, commonly associated with the presence of domestic and wild rodents. Human cases of murine typhus in the state of Yucatán are frequent. However, there is no evidence of the presence of Rickettsia typhi in mammals or vectors in Yucatán. The presence of Rickettsia in rodents and their ectoparasites was evaluated in a small municipality of Yucatán using the conventional polymerase chain reaction technique and sequencing. The study only identified the presence of Rickettsia typhi in blood samples obtained from Rattus rattus and it reported, for the first time, the presence of R. felis in the flea Polygenis odiosus collected from Ototylomys phyllotis rodent. Additionally, Rickettsia felis was detected in the ectoparasite Ctenocephalides felis fleas parasitizing the wild rodent Peromyscus yucatanicus. This study’s results contributed to a better knowledge of Rickettsia epidemiology in Yucatán.

Download Full-text

First Molecular Evidence of Pathogens in Fleas Collected from Dogs in Northern Vietnam

Pathogens ◽

10.3390/pathogens10091185 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1185

Author(s):

Thom Do ◽

Tawin Inpankaew ◽

Duc Hieu Duong ◽

Khanh Linh Bui

Keyword(s):

Molecular Detection ◽

Zoonotic Diseases ◽

Domestic Dogs ◽

Molecular Evidence ◽

International Literature ◽

Risk Of Infection ◽

Rickettsia Felis ◽

Northern Vietnam ◽

Mycoplasma Wenyonii ◽

Wide Range

Fleas are considered as hosts for a wide range of pathogens that cause emerging and re-emerging zoonotic diseases worldwide. Data on fleas and flea-borne pathogens (FBPs) in the international literature are limited in Vietnam. This study aimed to investigate the species of fleas and the presence of pathogens of interest in fleas in northern Vietnam using PCR and sequence analysis. Out of 200 dogs enrolled in this study, 20% were infested by the flea species Ctenocephalides felis felis. In total, 62 fleas (35 females and 27 males) collected from domestic dogs were molecularly screened for the detection of pathogens. Out of the screened fleas, 39 were positive for Rickettsia felis (62.9%), 9 for Candidatus Mycoplasma hemobos (14.52%), and 6 for Mycoplasma wenyonii (9.68%). This study shows the first molecular detection of the above-mentioned pathogens in fleas collected from the studied areas and the potential risk of infection with examined FBPs in northern Vietnam.

Download Full-text

Shell-vial culture and real-time PCR applied to Rickettsia typhi and Rickettsia felis detection

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-015-1925-z ◽

2015 ◽

Vol 31 (11) ◽

pp. 1747-1754 ◽

Cited By ~ 5

Author(s):

Ferran Segura ◽

Immaculada Pons ◽

Júlia Pla ◽

María-Mercedes Nogueras

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Rickettsia Typhi ◽

Rickettsia Felis ◽

Shell Vial

Download Full-text

Seroepidemiological study of Rickettsia felis, Rickettsia typhi, and Rickettsia conorii infection among the population of southern Spain

European Journal of Clinical Microbiology & Infectious Diseases ◽

10.1007/s10096-006-0147-6 ◽

2006 ◽

Vol 25 (6) ◽

pp. 375-381 ◽

Cited By ~ 40

Author(s):

M. Bernabeu-Wittel ◽

M. D. Toro ◽

M. M. Nogueras ◽

M. A. Muniain ◽

N. Cardeñosa ◽

...

Keyword(s):

Southern Spain ◽

Rickettsia Conorii ◽

Rickettsia Typhi ◽

Rickettsia Felis

Download Full-text

Molecular Detection of Rickettsia felis in Different Flea Species from Caldas, Colombia

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.12-0698 ◽

2013 ◽

Vol 89 (3) ◽

pp. 453-459 ◽

Cited By ~ 14

Author(s):

Alejandro Ramírez-Hernández ◽

Gustavo Valbuena ◽

Carolina Vélez ◽

Viviana Montoya ◽

Alejandra Martínez ◽

...

Keyword(s):

Molecular Detection ◽

Flea Species ◽

Rickettsia Felis

Download Full-text

Molecular Detection of Rickettsia felis in Haemaphysalis longicornis Ticks in Anhui Province of China

10.21203/rs.2.19614/v1 ◽

2019 ◽

Author(s):

Bo-Yu Liu ◽

Wan-Rong Luo ◽

Zhen Chen ◽

Cui-Ping Ren ◽

Ying Hong ◽

...

Keyword(s):

Sequence Analysis ◽

16S Rrna ◽

Molecular Detection ◽

Protein Gene ◽

Febrile Illness ◽

Anhui Province ◽

Rrna Gene ◽

Haemaphysalis Longicornis ◽

Rickettsia Felis ◽

Questing Ticks

Abstract BackgroundRickettsia felis causes febrile illness in humans. There is no information on the organism in Anhui Province of China.MethodsQuesting ticks were collected from Anhui Province of China and tick DNA was used as template for nested PCR (or real-time PCR) to amplify Rickettsia felis with 16S rRNA gene (rrs), 17kDa protein gene and orfB gene.ResultsPCR results indicated that 0.22% (2/907) ticks were positive to R. felis. Sequence analysis of the partial rrs and 17 kDa protein genes showed R. felis in ticks was highly homologous with R.felis URRWXCal2 with similarity of 98.91% and 99%, respectively.ConclusionsOur study demonstrated the first evidence of R. felis in H. longicornis ticks from Anhui Province of China, suggesting that a potential transmission of R. felis to humans through tick bite in China.

Download Full-text